Anti-APE1 antibody [CPTC-APEX1-2] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-APE1 antibody [CPTC-APEX1-2] - BSA and Azide free (AB268193)

Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling APE1 with ab268072 at 2 μg/ml followed by Goat anti-Mouse IgG-CF488 (Green). Phalloidin CF640 stains the membrane red.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268072)

• Flow Cyt

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Flow Cytometry - Anti-APE1 antibody [CPTC-APEX1-2] - BSA and Azide free (AB268193)

Flow Cytometryetric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for APE1 using ab268072. Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268072)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody [CPTC-APEX1-2] - BSA and Azide free (AB268193)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for APE1 using ab268072 at 2 μg/ml in immunohistochemical analysis

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268072)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody [CPTC-APEX1-2] - BSA and Azide free (AB268193)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for APE1 using ab268072 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268072)

• WB

Unknown

Western blot - Anti-APE1 antibody [CPTC-APEX1-2] - BSA and Azide free (AB268193)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268072)

All lanes:

Western blot - Anti-APE1 antibody [CPTC-APEX1-2] (<a href='/en-us/products/primary-antibodies/ape1-antibody-cptc-apex1-2-ab268072'>ab268072</a>) at 2 µg/mL

Lane 1:

A431 (Human epidermoid carcinoma cell line) cell lysate

Lane 2:

A549 (Human lung carcinoma cell line) cell lysate

Lane 3:

PC3 (Human prostate adenocarcinoma cell line) cell lysate

Lane 4:

HAP1 (Human cell line) cell lysate

Lane 5:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 35 kDa

false

• Protein Array

Unknown

Protein Array - Anti-APE1 antibody [CPTC-APEX1-2] - BSA and Azide free (AB268193)

Protein Array containing more than 19,000 full-length human proteins using ab268072.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268072)