AB59469

Anti-Apelin antibody

Name: Anti-Apelin antibody (ab59469) | Abcam
Brand: Abcam Limited
SKU: AB59469
Price: 535 USD
Availability: InStock

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(22 Publications)

Rabbit Polyclonal Apelin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human APLN.

View Alternative Names

APEL, APLN, Apelin, APJ endogenous ligand

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody (AB59469)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody (AB59469)

ab59469 (1 : 500) staining Apelin in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secreted regions of cells in the basal uterine glands.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human APLN. The exact immunogen used to generate this antibody is proprietary information.

Q9ULZ1

Specificity

This antibody reacts specifically with 8,5 kDa of human APLN protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apelin also known as APLN is a peptide ligand with a mass of approximately 77 amino acids. It has gained attention in scientific research due to its interaction with the G-protein coupled receptor named APJ (Apelin receptor). Apelin expresses widely in various tissues including heart lung kidney and gastrointestinal tract. This widespread expression suggests it plays roles in multiple physiological processes.

Biological function summary

Apelin acts as an important regulator of cardiovascular functions and fluid homeostasis. It modulates blood pressure and cardiac contractility indicating its role in maintaining cardiovascular health. Apelin operates independently but interacts closely with its receptor APJ forming a functional complex that initiates various intracellular signaling mechanisms. Researchers have noted its significant effects on the vascular system and potential involvement in angiogenesis.

Pathways

Apelin participates in the renin-angiotensin system and influences the nitric oxide signaling pathway. These pathways are important for maintaining vascular tone and pressure as well as for ensuring proper fluid balance in the body. Apelin's actions appear related to the angiotensin II type 1 receptor and endothelial nitric oxide synthase affecting their activity to achieve desired physiological outcomes. This involvement illustrates how apelin bridges different signaling networks to regulate homeostasis.

Apelin has connections to cardiovascular disorders and metabolic syndrome. High apelin levels have been observed in heart failure indicating its compensatory role in maintaining cardiovascular function. Additionally disruptions in apelin signaling may link to obesity-related diabetes given its effects on insulin sensitivity and energy metabolism. The interplay between apelin APJ and angiotensin II type 1 receptor highlights its potential as a therapeutic target for managing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endogenous ligand for the apelin receptor (APLNR) (PubMed : 10525157). Drives internalization of the apelin receptor (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity).. (Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection (PubMed : 11090199). Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR (PubMed : 11090199). Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives (PubMed : 11090199).

See full target information APLN

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 18:1379658 PubMed38803685

2024

Expression of the apelin receptor, a novel potential therapeutic target, and its endogenous ligands in diverse stem cell populations in human glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas L Williams,Peter Nwokoye,Rhoda E Kuc,Kieran Smith,Anna L Paterson,Kieren Allinson,Janet J Maguire,Anthony P Davenport

Pediatric blood & cancer 71:e30989 PubMed38602300

2024

Serum apelin as a potential biomarker for infantile hemangiomas.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Chen,Yunxuan Zhang,Sili Ni,Liuqing Yang,Jiwei Li,Xingang Yuan,Meng Chen,Jing Liu,Xiaoyan Luo,Yimin Xie,Hua Wang

Journal of clinical medicine 12: PubMed37048724

2023

Comparative Transcriptomic Analysis of Archival Human Vestibular Schwannoma Tissue from Patients with and without Tinnitus.

Applications

Unspecified application

Species

Unspecified reactive species

Krishna Bommakanti,Richard Seist,Phanidhar Kukutla,Murat Cetinbas,Shelley Batts,Ruslan I Sadreyev,Anat Stemmer-Rachamimov,Gary J Brenner,Konstantina M Stankovic

Frontiers in cell and developmental biology 9:628463 PubMed33738284

2021

Apelin Rejuvenates Aged Human Mesenchymal Stem Cells by Regulating Autophagy and Improves Cardiac Protection After Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Chengling Zhao,Guojun Jiang,Bei Hu,Huifeng Zheng,Yimei Hong,Zhen Cui,Linli Shi,Xin Li,Fang Lin,Yue Ding,Lu Wei,Mimi Li,Xiaoting Liang,Yuelin Zhang

Investigative ophthalmology & visual science 61:47 PubMed32729912

2020

The Role of Apelin/APJ in a Mouse Model of Oxygen-induced Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Feng,Li Chen,Yanrong Jiang,Yong Tao

Neuroscience letters 728:134948 PubMed32278025

2020

Apelin protects auditory cells from cisplatin-induced toxicity in vitro by inhibiting ROS and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Yin,Hui Zhang,Youhua Kong,Chunmei Wang,Yan Guo,Yang Gao,Lili Yuan,Xinxin Yang,Jing Chen

American journal of physiology. Renal physiology 318:F1122-F1135 PubMed32174138

2020

ELABELA antagonizes intrarenal renin-angiotensin system to lower blood pressure and protects against renal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanming Xu,Fei Wang,Yanting Chen,Shiying Xie,Danielle Sng,Bruno Reversade,Tianxin Yang

Biotechnic & histochemistry : official publication 95:27-36 PubMed31264472

2019

Tumor apelin immunoreactivity is correlated with body mass index in ovarian high grade serous carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

I Unal,I R Khiavi,G E Tasar,D Goksuluk,G Boyraz,N Ozgul,A Usubutun,S Yuruker,N D Zeybek

Thoracic cancer 10:1419-1430 PubMed31070305

2019

MicroRNA-195 suppresses the progression of lung adenocarcinoma by directly targeting apelin.

Applications

Unspecified application

Species

Unspecified reactive species

Yongchun Zhou,Ming Zhao,Yaxi Du,Yajie Liu,Guangqiang Zhao,Lianhua Ye,Quan Li,Hongsheng Li,Xiaoxiong Wang,Xin Liu,Yinjin Guo,Junxi Liu,Yunchao Huang

Disease markers 2019:4586405 PubMed30984306

2019

High Apelin Level Indicates a Poor Prognostic Factor in Muscle-Invasive Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Long Yang,Yan-Lei Li,Xiao-Qing Li,Zheng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Apelin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody (AB59469)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

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