AB230536

Anti-Apelin antibody - C-terminal

Name: Anti-Apelin antibody - C-terminal (ab230536) | Abcam
Brand: Abcam Limited
SKU: AB230536
Price: 605 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal Apelin antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human APLN conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

APEL, APLN, Apelin, APJ endogenous ligand

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (AB230536)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (AB230536)

Formalin-fixed, paraffin-embedded human testis tissue stained for Apelin using ab230536 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (AB230536)

Formalin-fixed, paraffin-embedded human lung tissue stained for Apelin using ab230536 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Apelin antibody - C-terminal (AB230536)

All lanes:

Western blot - Anti-Apelin antibody - C-terminal (ab230536) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 9 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human APLN conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9ULZ1

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apelin also known as APLN is a peptide ligand with a mass of approximately 77 amino acids. It has gained attention in scientific research due to its interaction with the G-protein coupled receptor named APJ (Apelin receptor). Apelin expresses widely in various tissues including heart lung kidney and gastrointestinal tract. This widespread expression suggests it plays roles in multiple physiological processes.

Biological function summary

Apelin acts as an important regulator of cardiovascular functions and fluid homeostasis. It modulates blood pressure and cardiac contractility indicating its role in maintaining cardiovascular health. Apelin operates independently but interacts closely with its receptor APJ forming a functional complex that initiates various intracellular signaling mechanisms. Researchers have noted its significant effects on the vascular system and potential involvement in angiogenesis.

Pathways

Apelin participates in the renin-angiotensin system and influences the nitric oxide signaling pathway. These pathways are important for maintaining vascular tone and pressure as well as for ensuring proper fluid balance in the body. Apelin's actions appear related to the angiotensin II type 1 receptor and endothelial nitric oxide synthase affecting their activity to achieve desired physiological outcomes. This involvement illustrates how apelin bridges different signaling networks to regulate homeostasis.

Apelin has connections to cardiovascular disorders and metabolic syndrome. High apelin levels have been observed in heart failure indicating its compensatory role in maintaining cardiovascular function. Additionally disruptions in apelin signaling may link to obesity-related diabetes given its effects on insulin sensitivity and energy metabolism. The interplay between apelin APJ and angiotensin II type 1 receptor highlights its potential as a therapeutic target for managing these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Endogenous ligand for the apelin receptor (APLNR) (PubMed : 10525157). Drives internalization of the apelin receptor (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity).. (Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection (PubMed : 11090199). Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR (PubMed : 11090199). Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives (PubMed : 11090199).

See full target information APLN

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

QJM : monthly journal of the Association of Physicians 116:197-204 PubMed36200913

2022

Apelin as a new therapeutic target for COVID-19 treatment.

Applications

Unspecified application

Species

Unspecified reactive species

J Park,M-Y Park,Y Kim,Y Jun,U Lee,C-M Oh

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apelin antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (AB230536)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody - C-terminal (AB230536)

Western blot - Anti-Apelin antibody - C-terminal (AB230536)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise