AB133624

Anti-Apelin antibody [EPNCIR154]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Apelin antibody. Suitable for ELISA and reacts with Human samples. Cited in 1 publication.

View Alternative Names

APEL, APLN, Apelin, APJ endogenous ligand

1 Images

ELISA - Anti-Apelin antibody [EPNCIR154] (AB133624)

ELISA

Unknown

ELISA - Anti-Apelin antibody [EPNCIR154] (AB133624)

ELISA assay using ab133624 shows the relationship between concentration of antibody and optical density readings.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR154

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apelin also known as APLN is a peptide ligand with a mass of approximately 77 amino acids. It has gained attention in scientific research due to its interaction with the G-protein coupled receptor named APJ (Apelin receptor). Apelin expresses widely in various tissues including heart lung kidney and gastrointestinal tract. This widespread expression suggests it plays roles in multiple physiological processes.

Biological function summary

Apelin acts as an important regulator of cardiovascular functions and fluid homeostasis. It modulates blood pressure and cardiac contractility indicating its role in maintaining cardiovascular health. Apelin operates independently but interacts closely with its receptor APJ forming a functional complex that initiates various intracellular signaling mechanisms. Researchers have noted its significant effects on the vascular system and potential involvement in angiogenesis.

Pathways

Apelin participates in the renin-angiotensin system and influences the nitric oxide signaling pathway. These pathways are important for maintaining vascular tone and pressure as well as for ensuring proper fluid balance in the body. Apelin's actions appear related to the angiotensin II type 1 receptor and endothelial nitric oxide synthase affecting their activity to achieve desired physiological outcomes. This involvement illustrates how apelin bridges different signaling networks to regulate homeostasis.

Apelin has connections to cardiovascular disorders and metabolic syndrome. High apelin levels have been observed in heart failure indicating its compensatory role in maintaining cardiovascular function. Additionally disruptions in apelin signaling may link to obesity-related diabetes given its effects on insulin sensitivity and energy metabolism. The interplay between apelin APJ and angiotensin II type 1 receptor highlights its potential as a therapeutic target for managing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endogenous ligand for the apelin receptor (APLNR) (PubMed : 10525157). Drives internalization of the apelin receptor (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity).. (Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection (PubMed : 11090199). Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR (PubMed : 11090199). Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives (PubMed : 11090199).

See full target information APLN

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Analyst 145:5180-5193 PubMed32567629

2020

Gold nanoparticle-streptavidin conjugates for rapid and efficient screening of aptamer function in lateral flow sensors using novel CD4-binding aptamers identified through Crossover-SELEX.

Applications

Unspecified application

Species

Unspecified reactive species

Tamika Fellows,Lance Ho,Shane Flanagan,Ronen Fogel,Dupe Ojo,Janice Limson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apelin antibody [EPNCIR154]

ELISA - Anti-Apelin antibody [EPNCIR154] (AB133624)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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