AB228668

Anti-APG5L/ATG5 antibody

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(23 Publications)

Rabbit Polyclonal APG5L/ATG5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 23 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATG5.

View Alternative Names

APG5L, ASP, ATG5, Autophagy protein 5, APG5-like, Apoptosis-specific protein

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody (AB228668)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody (AB228668)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for APG5L/ATG5 (green) using ab228668 (1/500 dilution) in ICC/IF. Cells were treated with 3 μM thapsigargin 16 hours (right panels) and mock (left panels) and were fixed in ice-cold methanol for 10 minutes, permeabilized with cooled acetone for 2 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody (AB228668)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody (AB228668)

Paraffin-embedded mouse brain tissue stained for APG5L/ATG5 with ab228668 at 1/400 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-APG5L/ATG5 antibody (AB228668)

12% SDS-PAGE

All lanes:

Western blot - Anti-APG5L/ATG5 antibody (ab228668) at 1/1000 dilution

Lane 1:

NT2/D1 (human embryonal carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 32 kDa

true

Supplier Data

Western blot - Anti-APG5L/ATG5 antibody (AB228668)

15% SDS-PAGE

All lanes:

Western blot - Anti-APG5L/ATG5 antibody (ab228668) at 1/1000 dilution

Lane 1:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 50 μM chloroquine for 24 hours, whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 32 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ATG5. The exact immunogen used to generate this antibody is proprietary information.

Q9H1Y0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APG5L commonly known as ATG5 or ATG5 protein plays a significant role in autophagy. This molecular component has a molecular weight of approximately 32 kDa. ATG5 is expressed in multiple tissue types with a higher expression profile in immune-related cells. The protein is essential for the formation of the autophagosome as it conjugates with ATG12 to form a complex that is critical for autophagosome membrane elongation.

Biological function summary

ATG5 plays an important part in the cellular process of autophagy affecting the degradation and recycling of cellular components. It is a part of a complex with ATG12 and ATG16L1 which facilitates the membrane building of the autophagosome. This protein is important for maintaining cellular homeostasis allowing the removal of damaged proteins and organelles and is involved in responses to stress and starvation.

Pathways

ATG5 is an important component in the autophagy pathway and is involved in cellular maintenance processes. This pathway is vital for the regulation of cell growth and metabolism. ATG5 works closely with other autophagy-related proteins such as LC3. The ATG5-ATG12 complex helps in the lipidation of LC3 which is critical for autophagosome maturation connecting its function to the mTOR signaling pathway that regulates many cellular processes.

Disruption in ATG5 function is linked to neurodegenerative diseases and cancer. For instance dysregulation of autophagy due to ATG5 dysfunction may result in the progression of neurodegenerative disorders like Alzheimer's disease. Additionally in certain cancers altered autophagy can influence the survival of cancer cells. The interplay between ATG5 and other proteins like p53 and Bcl-2 is under investigation to understand its role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity).. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.. (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed : 17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established (PubMed : 19666601).

See full target information ATG5

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40141229

2025

mTOR-Mediated Autophagy Regulates Cadmium-Induced Kidney Injury via Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Hu,Kui Wang,Jie Xu,Guohuan Wan,Yiyi Zhao,Yajing Chen,Kangfeng Jiang,Xiaobing Li

Heliyon 10:e33829 PubMed39816371

2025

Quercetin attenuated ropivacaine-induced neurotoxicity in SH-SY5Y cells through upregulating Pim1 and enhancing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghai Liu,Ling Ren,Yangyang Sun,Jing Yan,Gu Gong

Animal bioscience 37:284-294 PubMed37905320

2023

Apoptosis and autophagy of muscle cell during pork postmortem aging.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmei Li,Xialian Yin,Panpan Xue,Feng Wang,Ruilong Song,Qi Song,Jiamin Su,Haifeng Zhang

Autophagy 19:1239-1257 PubMed36109708

2022

Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both and .

Applications

Unspecified application

Species

Unspecified reactive species

Shan-Ying Wu,Hung-Tsung Wu,Yi-Ching Wang,Chih-Jen Chang,Yan-Shen Shan,Shang-Rung Wu,Yen-Chi Chiu,Chia-Lang Hsu,Hsueh-Fen Juan,Kai-Ying Lan,Chi-Wen Chu,Ying-Ray Lee,Sheng-Hui Lan,Hsiao-Sheng Liu

Frontiers in immunology 13:934819 PubMed36148236

2022

induces autophagy in bovine mammary epithelial cells the HIF-1α and AMPKα/ULK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenpeng Zhao,Maolin Xu,Herman W Barkema,Xiaochen Xie,Yushan Lin,Sohrab Khan,John P Kastelic,Dong Wang,Zhaoju Deng,Bo Han

Biomedicines 10: PubMed35884836

2022

Ubiquitin-Specific Protease 6 n-Terminal-like Protein (USP6NL) and the Epidermal Growth Factor Receptor (EGFR) Signaling Axis Regulates Ubiquitin-Mediated DNA Repair and Temozolomide-Resistance in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

I-Chang Su,Yu-Kai Su,Hao-Yu Chuang,Vijesh Kumar Yadav,Syahru Agung Setiawan,Iat-Hang Fong,Chi-Tai Yeh,Hui-Chuan Huang,Chien-Min Lin

Journal of virology 96:e0024422 PubMed35319226

2022

PPRV-Induced Autophagy Facilitates Infectious Virus Transmission by the Exosomal Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yangli Wan,Yan Chen,Ting Wang,Bao Zhao,Wei Zeng,Leyan Zhang,Yanming Zhang,Shengyan Cao,Jingyu Wang,Qinghong Xue,Xuefeng Qi

Adipocyte 10:558-573 PubMed34743657

2021

Association of cell death mechanisms and fibrosis in visceral white adipose tissue with pathological alterations in the liver of morbidly obese patients with NAFLD.

Applications

Unspecified application

Species

Unspecified reactive species

Anna-Sophia Leven,Robert K Gieseler,Martin Schlattjan,Thomas Schreiter,Marco Niedergethmann,Theodor Baars,Hideo A Baba,Mustafa K Özçürümez,Jan-Peter Sowa,Ali Canbay

Frontiers in immunology 12:671511 PubMed34054847

2021

Sildenafil Alleviates Murine Experimental Autoimmune Encephalomyelitis by Triggering Autophagy in the Spinal Cord.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo Duarte-Silva,Shyrlene Meiry da Rocha Araújo,Wilma Helena Oliveira,Deniele Bezerra Lós,Amanda Pires Bonfanti,Gabriela Peron,Livia de Lima Thomaz,Liana Verinaud,Christina Alves Peixoto

Cell death & disease 11:771 PubMed32943609

2020

Wnt/β-catenin signaling pathway induces autophagy-mediated temozolomide-resistance in human glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jin Yun,Sangwoo Kim,Jer-Tsong Hsieh,Seung Tae Baek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-APG5L/ATG5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody (AB228668)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody (AB228668)

Western blot - Anti-APG5L/ATG5 antibody (AB228668)

Western blot - Anti-APG5L/ATG5 antibody (AB228668)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise