Anti-APG5L/ATG5 antibody [EPR1755(2)]
- 20ul selling size
- RabMAb
- Recombinant
- KO Validated
- What is this?
4
(7 Reviews)
|
(285 Publications)
Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) is a rabbit monoclonal antibody detecting APG5L/ATG5 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 190 publications
View Alternative Names
APG5L, ASP, ATG5, Autophagy protein 5, APG5-like, Apoptosis-specific protein
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Unpurified ab108327, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human hepatocellular carcinoma tissue by Immunohistochemistry.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Unpurified ab108327, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human ovarian adenocarcinoma tissue by Immunohistochemistry.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab180327 at a working dilution of 1/150. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Immunofluorescence staining of MCF7 cells with purified ab108327 at a working dilution of 1/150, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108327 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
- IP
Unknown
Immunoprecipitation - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
ab180327 (purified) at 1/20 immunoprecipitating CRSP8 in 10 μg PC-12 whole cell lysate (Lanes 1 and 2, observed at 55 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution. Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST
All lanes:
Immunoprecipitation - Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327)
Predicted band size: 32 kDa
false
- WB
Lab
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
False colour image of Western blot : Anti-APG5L/ATG5 antibody [EPR1755(2)] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108327 was shown to bind specifically to APG5L/ATG5. A band was observed at 50 kDa in wild-type THP-1 cell lysates with no signal observed at this size in ATG5 knockout cell line ab277835 (knockout cell lysate ab290722). To generate this image, wild-type and ATG5 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) at 1/1000 dilution
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
ATG5 knockout THP-1 cell lysate at 20 µg
Lane 3:
U-87 MG cell lysate at 20 µg
Lane 4:
HeLa cell lysate at 20 µg
Secondary
Lanes 1 - 4:
Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4:
Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Observed band size: 50 kDa
false
- WB
Lab
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) at 1/10000 dilution
All lanes:
Raji cell lysate at 20 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 55 kDa
false
- WB
Lab
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : APG5L/ATG5 knockout HAP1 cell lysate (20 μg)
Lane 3 : Raji cell lysate (20 μg)
Lane 4 : Jeg-3 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab108327 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108327 was shown to specifically react with APG5L/ATG5 when APG5L/ATG5 knockout samples were used. Wild-type and APG5L/ATG5 knockout samples were subjected to SDS-PAGE. ab108327 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327)
Predicted band size: 32 kDa
false
- WB
Unknown
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Secondary antibody - anti-rabbit HRP (ab6721)
All lanes:
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) at 1/1000 dilution
Lane 1:
Raji cell lysate at 10 µg
Lane 2:
HeLa cell lysate at 10 µg
Lane 3:
HT-1080 cell lysate at 10 µg
Lane 4:
Human fetal kidney cell lysate at 10 µg
Lane 5:
C6 cell lysate at 10 µg
Lane 6:
Raw264.7 cell lysate at 10 µg
Lane 7:
PC-12 cell lysate at 10 µg
Lane 8:
NIH3T3 cell lysate at 10 µg
Predicted band size: 32 kDa
false
- WB
Lab
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) at 1/2000 dilution
All lanes:
human fetal kidney at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 102 kDa,32 kDa
Observed band size: 32 kDa,55 kDa
false
- WB
Lab
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) at 1/2000 dilution
All lanes:
HT-1080 cell lysate at 20 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 55 kDa
false
- WB
Lab
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) at 1/10000 dilution
Lane 1:
C6 cell lysate at 20 µg
Lane 2:
PC-12 cell lysate at 20 µg
Lane 3:
NIH/3T3 cell lysate at 20 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 55 kDa
false
Related conjugates and formulations (1)
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Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of APG5L/ATG5?
Anti-APG5L/ATG5 [EPR1755(2)] (ab108327) specifically detects a band for APG5L/ATG5 (UniProt: Q9H1Y0) at a molecular weight of 32kDa.
Trusted by the scientific community
Anti-APG5L/ATG5 [EPR1755(2)] (ab108327) was first used in a scientific publication in 2012 and has been cited over 190 times in peer-reviewed journals.
Reviewed by scientists
Anti-APG5L/ATG5 [EPR1755(2)] (ab108327) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) has been confirmed by Western blot testing in ATG5 Knockout HAP1 cell line, ab277835.
Other related products
We have a range of other formats of antibody clone [EPR1755(2)] also available for your convenience: ab108327, HRP - ab199560, Carrier free - ab221604, ab307842, Carrier free - ab307843
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATG5 plays an important part in the cellular process of autophagy affecting the degradation and recycling of cellular components. It is a part of a complex with ATG12 and ATG16L1 which facilitates the membrane building of the autophagosome. This protein is important for maintaining cellular homeostasis allowing the removal of damaged proteins and organelles and is involved in responses to stress and starvation.
Pathways
ATG5 is an important component in the autophagy pathway and is involved in cellular maintenance processes. This pathway is vital for the regulation of cell growth and metabolism. ATG5 works closely with other autophagy-related proteins such as LC3. The ATG5-ATG12 complex helps in the lipidation of LC3 which is critical for autophagosome maturation connecting its function to the mTOR signaling pathway that regulates many cellular processes.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (285)
Recent publications for all applications. Explore the full list and refine your search
Research and reports in urology 17:383-399 PubMed41048480
2025
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Autophagy :1-16 PubMed40851209
2025
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American journal of cancer research 15:2949-2969 PubMed40814362
2025
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Scientific reports 15:29484 PubMed40796594
2025
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Journal of traditional and complementary medicine 15:434-445 PubMed40677544
2025
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Acta biochimica et biophysica Sinica : PubMed40567051
2025
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Frontiers in oncology 15:1531420 PubMed40519291
2025
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Nature communications 16:5261 PubMed40480980
2025
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Discover oncology 16:687 PubMed40338396
2025
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Scientific reports 15:15572 PubMed40320451
2025
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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