Rabbit Recombinant Monoclonal APG5L/ATG5 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 16 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98137.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604)")
![Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunocytochemistry-immunofluorescence-img59924.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604)")
![Immunoprecipitation - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunoprecipitation-img58280.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134369.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7048.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (AB221604)")
Publications
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- International journal of molecular medicine 49:2022Beclin‑1 exerts protective effects against cerebral ischemia‑reperfusion injury by promoting DNA damage repair through a non‑autophagy‑dependent regulatory mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongcheng Luo,Deyou Huang,Xionglin Tang,Yu Liu,Qisheng Luo,Chunhong Liu,Huatuo Huang,Wencheng Chen,Zhongquan QiPubMed 35266018
- Cell death discovery 8:592022YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangfei Xue,Xiaoting Tian,Congcong Zhang,Yayou Miao,Yikun Wang,Yingxiu Peng,Shiyu Qiu,Hong Wang,Jiangtao Cui,Leiqun Cao,Fenyong Sun,Yongxia Qiao,Xiao ZhangPubMed 35149670
- Oncology letters 20:572020Erastin triggers autophagic death of breast cancer cells by increasing intracellular iron levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengxin Li,Xuanzhong Wang,Shan Lu,Chuan He,Chongcheng Wang,Lei Wang,Xinyu Wang,Pengfei Ge,Dong SongPubMed 32793311
- The international journal of biochemistry & cell b 111:63-712019MicroRNA-137 inhibits autophagy and chemosensitizes pancreatic cancer cells by targeting ATG5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Chao Wang et. al.PubMed 30710750
- The American journal of Chinese medicine 44:1393-14122016Tetrahydroxystilbene Glucoside Inhibits Excessive Autophagy and Improves Microvascular Endothelial Dysfunction in Prehypertensive Spontaneously Hypertensive Rats.
- Autophagy 12:1776-17902016Metastatic risk and resistance to BRAF inhibitors in melanoma defined by selective allelic loss of ATG5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría García-Fernández,Panagiotis Karras,Agnieszka Checinska,Estela Cañón,Guadalupe T Calvo,Gonzalo Gómez-López,Metehan Cifdaloz,Angel Colmenar,Luis Espinosa-Hevia,David Olmeda,María S SoengasPubMed 27464255
- Developmental cell 37:337-3492016The Autophagy Machinery Controls Cell Death Switching between Apoptosis and Necroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegan L Goodall,Brent E Fitzwalter,Shadi Zahedi,Min Wu,Diego Rodriguez,Jean M Mulcahy-Levy,Douglas R Green,Michael Morgan,Scott D Cramer,Andrew ThorburnPubMed 27219062
- Autophagy 12:1129-522016A novel tumor-promoting mechanism of IL6 and the therapeutic efficacy of tocilizumab: Hypoxia-induced IL6 is a potent autophagy initiator in glioblastoma via the p-STAT3-MIR155-3p-CREBRF pathway.
- Autophagy 12:907-162016Loss of autophagy enhances MIF/macrophage migration inhibitory factor release by macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacinta P W Lee et. al.PubMed 27163877
- Autophagy 12:976-982016Persistent activation of autophagy in kidney tubular cells promotes renal interstitial fibrosis during unilateral ureteral obstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan J Livingston et. al.PubMed 27123926
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Predicted | Expected | Predicted | Predicted |
| Rat | Tested | Expected | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 product for Alternative Version
Target data
Function
Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity). May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD. (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established (PubMed:19666601).
Alternative names
APG5L, ASP, ATG5, Autophagy protein 5, APG5-like, Apoptosis-specific protein
Recommended products
Rabbit Recombinant Monoclonal APG5L/ATG5 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 16 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1755(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab221604 is the carrier-free version of Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
APG5L commonly known as ATG5 or ATG5 protein plays a significant role in autophagy. This molecular component has a molecular weight of approximately 32 kDa. ATG5 is expressed in multiple tissue types with a higher expression profile in immune-related cells. The protein is essential for the formation of the autophagosome as it conjugates with ATG12 to form a complex that is critical for autophagosome membrane elongation.
- Biological function summary
ATG5 plays an important part in the cellular process of autophagy affecting the degradation and recycling of cellular components. It is a part of a complex with ATG12 and ATG16L1 which facilitates the membrane building of the autophagosome. This protein is important for maintaining cellular homeostasis allowing the removal of damaged proteins and organelles and is involved in responses to stress and starvation.
- Pathways
ATG5 is an important component in the autophagy pathway and is involved in cellular maintenance processes. This pathway is vital for the regulation of cell growth and metabolism. ATG5 works closely with other autophagy-related proteins such as LC3. The ATG5-ATG12 complex helps in the lipidation of LC3 which is critical for autophagosome maturation connecting its function to the mTOR signaling pathway that regulates many cellular processes.
- Associated diseases and disorders
Disruption in ATG5 function is linked to neurodegenerative diseases and cancer. For instance dysregulation of autophagy due to ATG5 dysfunction may result in the progression of neurodegenerative disorders like Alzheimer's disease. Additionally in certain cancers altered autophagy can influence the survival of cancer cells. The interplay between ATG5 and other proteins like p53 and Bcl-2 is under investigation to understand its role in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98137.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)
Unpurified Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human ovarian adenocarcinoma tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327).
![Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunocytochemistry-immunofluorescence-img59924.jpg "Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)")
Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)
Immunofluorescence staining of MCF7 cells with purified Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327 at a working dilution of 1/150, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327).
![Immunoprecipitation - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunoprecipitation-img58280.jpg "Immunoprecipitation - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)")
Immunoprecipitation - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)
Recombinant Human MED3 protein (denatured) ab180327 (purified) at 1/20 immunoprecipitating CRSP8 in 10 �g PC-12 whole cell lysate (Lanes 1 and 2, observed at 55 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327).
All lanes: Immunoprecipitation - Anti-APG5L/ATG5 antibody [EPR1755(2)] (Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327)
Predicted band size: 32 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134369.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)
Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified Recombinant Human MED3 protein (denatured) ab180327 at a working dilution of 1/150. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7048.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)
Unpurified Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327, at 1/100 dilution, staining APG5L/ATG5 in paraffin-embedded Human hepatocellular carcinoma tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327).
![Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--western-blot-img417819.jpg "Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)")
Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)
False colour image of Western blot: Anti-APG5L/ATG5 antibody [EPR1755(2)] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327 was shown to bind specifically to APG5L/ATG5. A band was observed at 50 kDa in wild-type THP-1 cell lysates with no signal observed at this size in ATG5 knockout cell line Human ATG5 knockout THP-1 cell line ab277835 (knockout cell lysate ab290722). To generate this image, wild-type and ATG5 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (Anti-APG5L/ATG5 antibody [EPR1755(2)] ab108327) at 1/1000 dilution
Lane 1: Wild-type THP-1 cell lysate at 20 µg
Lane 2: ATG5 knockout THP-1 cell lysate at 20 µg
Lane 3: U-87 MG cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
SecondaryLanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 50 kDa
![Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604), expandable thumbnail](https://content.abcam.com/products/images/apg5l-atg5-antibody-epr17552-bsa-and-azide-free-ab221604--western-blot-img417819.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)")
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