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AB236608

Anti-APH antibody

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(2 Publications)

Rabbit Polyclonal APH antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human APEH aa 200-500.

View Alternative Names

D3F15S2, D3S48E, DNF15S2, APEH, Acylamino-acid-releasing enzyme, AARE, Acyl-peptide hydrolase, Acylaminoacyl-peptidase, Oxidized protein hydrolase, APH, OPH

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APH antibody (AB236608)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APH antibody (AB236608)

Paraffin-embedded human colon cancer tissue stained for APH using ab236608 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-APH antibody (AB236608)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-APH antibody (AB236608)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ab236608 using ab236608 at 1/100 dilution in ICC/IF. Secondary antibody was an Alexa-Fluor®488-conjugated goat anti-rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APH antibody (AB236608)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APH antibody (AB236608)

Paraffin-embedded human renal tissue stained for APH using ab236608 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-APH antibody (AB236608)
  • WB

Supplier Data

Western blot - Anti-APH antibody (AB236608)

All lanes:

Western blot - Anti-APH antibody (ab236608) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Mouse liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human APEH aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

P13798

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein APH also known as Aminoglycoside Phosphotransferase or APH(2") serves a critical role in antibiotic resistance mechanisms by phosphorylating aminoglycoside antibiotics leading to inactivation. APH with a mass of approximately 31 kDa can be found in bacteria that are often resistant to several antibiotics. Although mainly located in prokaryotes certain modified forms also show expression in eukaryotic systems for research purposes.
Biological function summary

APH plays a significant role in cell survival by protecting bacteria from antibiotic stress a process necessary for maintaining membrane integrity under antibiotic pressure. This enzyme is not a part of a larger protein complex but exhibits highly specific interactions with aminoglycosides. It commonly associates with resistance mechanisms allowing bacteria to survive in otherwise lethal environments.

Pathways

APH significantly impacts aminoglycoside metabolic pathways and contributes to the overarching antibiotic resistance pathway. This phosphotransferase action is often coordinated alongside AAC (Aminoglycoside N-acetyltransferase) and ANT (Aminoglycoside O-nucleotidyltransferase) proteins. They collectively enable bacteria survival by modifying antibiotics preventing their effective binding to bacterial ribosomal targets.

APH presents a considerable threat due to its association with multidrug-resistant bacterial infections and urinary tract infections. The protein's role in resistance complicates treatment protocols and is integrated into bacterial adaptation strategies. Alongside proteins like AAC and ANT APH contributes to bacterial resilience making it a target of interest in studies focused on developing novel antibacterial treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Aminopeptidase involved in the degradation of N(alpha)-acylated peptides. Displays exopeptidase activity toward N-formyl and N-acetyl peptides with a preference for dipeptide substrates (By similarity). Catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus (PubMed : 10719179, PubMed : 1740429, PubMed : 2006156). It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser (By similarity). Hydrolyzes N-formylated peptides likely arising from N-terminal proteolytic cleavage of bacterial and mitochondrial proteins. May act sequentially with ACY1 in the degradation of N-acylated peptides : APEH first cleaves N-acylaminoacids from N-acylated peptides, then ACY1 further hydrolyzes the N-acylaminoacid into free aminoacid and a carboxylate (By similarity). Displays endopeptidase activity involved in the degradation of oxidized and glycated proteins (PubMed : 10719179).
See full target information APEH
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 11:25 PubMed40097405

2025

A decrease in Flavonifractor plautii and its product, phytosphingosine, predisposes individuals with phlegm-dampness constitution to metabolic disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Lingru Li,Tianxing Li,Xue Liang,Linghui Zhu,Yini Fang,Ling Dong,Yi Zheng,Xiaoxue Xu,Mingrui Li,Tianqi Cai,Fufangyu Zhao,Meiling Xin,Mingyan Shao,Yuanyuan Guan,Meiyi Liu,Fangli Li,Chenhong Zhang,Qi Wang,Wenlong Sun,Yanfei Zheng

International journal of stem cells 13:221-236 PubMed32323511

2020

Intratracheal Transplantation of Amnion-Derived Mesenchymal Stem Cells Ameliorates Hyperoxia-Induced Neonatal Hyperoxic Lung Injury via Aminoacyl-Peptide Hydrolase.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenghao Li,Xiangcui Gong,Dong Li,Xiaofei Yang,Qing Shi,Xiuli Ju
View all publications
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Product promise

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