Anti-APJ Receptor antibody [RP23040146]

• WB

Supplier Data

Western blot - Anti-APJ Receptor antibody [RP23040146] (AB308018)

Western blot analysis was performed on membrane enriched extracts (30 µg of lysate) of HepG2 (Lane 1), NTERA-2 (Lane 2), HEL.92.1.7 (Lane 3), K562 (Lane 4), HeLa (Lane 8) and tissue extracts of Mouse Liver (Lane 5), Mouse Testis (Lane 6) Rat Testis (Lane 7) and Mouse Lung (Lane 9). The blots were probed with ab308018 at 1-2 µg/mL dilution and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution. A 43 kDa band corresponding to APLNR protein was observed. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-APJ Receptor antibody [RP23040146] (ab308018) at 1 µg/mL

Lane 1:

HepG2 membrane enriched extracts at 30 µg

Lane 2:

NTERA-2 membrane enriched extracts at 30 µg

Lane 3:

HEL 92.1.7 membrane enriched extracts at 30 µg

Lane 4:

K562 membrane enriched extracts at 30 µg

Lane 5:

Mouse liver tissue extract at 30 µg

Lane 6:

Rat liver tissue extract at 30 µg

Lane 7:

Mouse stomach tissue extract at 30 µg

Lane 8:

Mouse testis tissue extract at 30 µg

Lane 9:

Rat testis tissue extract at 30 µg

Lane 10:

HeLa membrane enriched extracts at 30 µg

Lane 11:

Mouse lung tissue extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Observed band size: 43 kDa

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