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AB196502

Anti-APLF antibody

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(1 Publication)

Rabbit Polyclonal APLF antibody. Suitable for WB, IHC-P and reacts with African green monkey, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human APLF.

View Alternative Names

C2orf13, PALF, XIP1, APLF, Aprataxin and PNK-like factor, Apurinic-apyrimidinic endonuclease APLF, PNK and APTX-like FHA domain-containing protein, XRCC1-interacting protein 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLF antibody (AB196502)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLF antibody (AB196502)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling APLF with ab196502 at 1/50 dilution. The image on the right has been pre-incubated with the blocking peptide.

Western blot - Anti-APLF antibody (AB196502)
  • WB

Supplier Data

Western blot - Anti-APLF antibody (AB196502)

All lanes:

Western blot - Anti-APLF antibody (ab196502) at 1/500 dilution

Lane 1:

HT29 cell extract

Lane 2:

HT29 cell extract with systhesized peptide

Predicted band size: 56 kDa

false

Western blot - Anti-APLF antibody (AB196502)
  • WB

Supplier Data

Western blot - Anti-APLF antibody (AB196502)

All lanes:

Western blot - Anti-APLF antibody (ab196502) at 1/500 dilution

Lane 1:

COS7 cell extracts with systhesized peptide

Lane 2:

COS7 cell extracts

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, African green monkey

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human APLF. The exact immunogen used to generate this antibody is proprietary information.

Q8IW19

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab196502 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APLF also known as Aprataxin and PNK-like Factor is a protein involved in DNA repair. The protein has a molecular mass of approximately 55 kDa. It expresses mainly in the nucleus of cells where it acts on various substrates involved in maintaining genomic stability. APLF possesses multiple domains enabling it to interact with many partners in the DNA damage response network.
Biological function summary

The APLF protein plays an essential role in the repair of DNA double-strand breaks. Its zinc finger motif facilitates the recruitment to sites of DNA damage where it functions as a histone chaperone. APLF often acts as part of a larger protein complex that includes DNA-PKcs which enhances the non-homologous end joining (NHEJ) pathway. These interactions allow for efficient processing and repair of breaks ensuring the continuity of genetic information.

Pathways

Several DNA repair processes involve APLF including the NHEJ and base excision repair (BER) pathways. In NHEJ APLF interacts with proteins such as XRCC4 which helps coordinate the repair of DNA ends. The role of APLF in BER assists in the correction of non-coding DNA damage commonly working alongside polymerase beta. These pathways together enable cells to maintain DNA integrity under conditions of stress or mutagenic exposures.

APLF has associations with neurodegenerative diseases and cancer. Its role in DNA repair links it to neurodegenerative diseases where DNA damage accumulation contributes to pathology. In addition altered expression of APLF may lead to defective DNA repair and promote oncogenesis. The interaction between APLF and key repair proteins like DNA-PKcs and XRCC4 further highlights its importance in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone chaperone involved in single-strand and double-strand DNA break repair (PubMed : 17353262, PubMed : 17396150, PubMed : 21211721, PubMed : 21211722, PubMed : 29905837, PubMed : 30104678). Recruited to sites of DNA damage through interaction with branched poly-ADP-ribose chains, a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions (PubMed : 17353262, PubMed : 17396150, PubMed : 21211721, PubMed : 30104678). Following recruitment to DNA damage sites, acts as a histone chaperone that mediates histone eviction during DNA repair and promotes recruitment of histone variant MACROH2A1 (PubMed : 21211722, PubMed : 29905837, PubMed : 30104678). Also has a nuclease activity : displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro (PubMed : 17353262, PubMed : 17396150). Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage (PubMed : 17353262, PubMed : 17396150). Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (PubMed : 21211721, PubMed : 23689425). Also acts as a negative regulator of cell pluripotency by promoting histone exchange (By similarity). Required for the embryo implantation during the epithelial to mesenchymal transition in females (By similarity).
See full target information APLF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research 38:292 PubMed31287003

2019

Rewiring E2F1 with classical NHEJ via APLF suppression promotes bladder cancer invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Christin Richter,Stephan Marquardt,Fanghua Li,Alf Spitschak,Nico Murr,Berdien A H Edelhäuser,George Iliakis,Brigitte M Pützer,Stella Logotheti
View all publications
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