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Mouse Recombinant Monoclonal APLP1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-APLP1 antibody [AP1] (AB273448), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (AB273448), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (AB273448), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (AB273448), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (AB273448), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIHC-Fr
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.

Alternative names

Amyloid beta precursor like protein 1, Amyloid beta (A4) precursor-like protein 1, Amyloid-like protein 1, APLP, APLP-1, APLP1

Recommended products

Mouse Recombinant Monoclonal APLP1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
AP1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

APLP1 also known as amyloid precursor-like protein 1 is a protein with a molecular mass of approximately 91 kDa. Researchers have observed its expression in the brain particularly in regions like the cortex and hippocampus. This protein shares structural similarities with the Amyloid Precursor Protein (APP) family and plays important roles in cellular functions through its protein domains and motifs.

Biological function summary

APLP1 functions by contributing to cell-cell adhesion and synaptic formation impacting neuronal development. It forms part of a larger protein complex that modulates these processes interacting with other proteins to maintain cellular communication. This protein's activity supports the stability and plasticity of synapses which is essential for proper neural network function.

Pathways

Proteins related to APLP1 participate notably in the Notch signaling and MAPK pathways. These pathways influence cell fate decisions growth and survival. APLP1 interacts with proteins such as APP and APLP2 both of which also engage in similar signaling pathways. This interconnectedness highlights APLP1's role in neurobiological processes essential for normal brain operation.

Associated diseases and disorders

Researchers link APLP1 to neurodegenerative disorders like Alzheimer's disease and autism spectrum disorders. Changes in APLP1 expression or function can affect amyloidogenic processing connecting it to APP and potentially leading to plaque formation a hallmark of Alzheimer's disease. Additionally this protein's relationship with synaptic function may contribute to the pathophysiology observed in autism spectrum disorders where alterations in synaptic communication are common.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-APLP1 antibody [AP1] (ab273448), expandable thumbnail

    Western blot - Anti-APLP1 antibody [AP1] (ab273448)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-APLP1 antibody [AP1] (ab273448) at 1/1000 dilution

    All lanes: Mouse brain cortex tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 72 kDa

    Observed band size: 97 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on human cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use secondary.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448)

    Immunohistochemical analysis of paraffin-embedded human cerebrum with Alzheimer's disease tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on human cerebrum with Alzheimer's disease is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use secondary.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on mouse cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use secondary.
    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on rat cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use secondary.
    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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