Anti-APLP1 antibody [EPR25737-10]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (AB291070)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling APLP1 with ab291070 at 1/5000 (0.209 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebrum (PMID : 9444352). The section was incubated with ab291070 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (AB291070)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling APLP1 with ab291070 at 1/5000 (0.209 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab291070 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (AB291070)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling APLP1 with ab291070 at 1/5000 (0.209 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab291070 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-APLP1 antibody [EPR25737-10] (AB291070)

Flow cytometric analysis of Mouse primary neuron cell cells labelling APLP1 with ab291070 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• WB

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Western blot - Anti-APLP1 antibody [EPR25737-10] (AB291070)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Samples are non-boiled as boiling may cause protein aggregates. The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 7494461; PMID : 18430897). Low expression : Liver, small intestine, colon cancer (PMID : 18430897).

All lanes:

Western blot - Anti-APLP1 antibody [EPR25737-10] (ab291070) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate

Lane 2:

Human liver tissue lysate

Lane 3:

Human small intestine tissue lysate

Lane 4:

Human colon cancer tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 97 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-APLP1 antibody [EPR25737-10] (AB291070)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Samples are non-boiled as boiling may cause protein aggregates. The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 7494461; PMID : 18430897). Low expression : Liver (PMID : 18430897 ).

All lanes:

Western blot - Anti-APLP1 antibody [EPR25737-10] (ab291070) at 1/1000 dilution

Lanes 1 and 7:

Mouse brain tissue lysate 20 µg

Lane 2:

Mouse cerebral cortex tissue lysate 20 µg

Lane 3:

Mouse liver tissue lysate 20 µg

Lane 4:

Rat brain tissue lysate 20 µg

Lane 5:

Rat cerebral cortex tissue lysate 20 µg

Lane 6:

Rat liver tissue lysate 20 µg

Lane 8:

Mouse brain tissue lysate treated with PNGase F 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 72 kDa,97 kDa,95 kDa

false

Exposure time: 26s