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Rabbit Monoclonal APLP1 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (AB291097), expandable thumbnail
  • Western blot - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (AB291097), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (AB291097), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (AB291097), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (AB291097), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow CytIP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Tested
Mouse
Tested
Tested
Not recommended
Not recommended
Tested
Tested
Rat
Tested
Tested
Not recommended
Not recommended
Expected
Expected

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.

Alternative names

Amyloid beta precursor like protein 1, Amyloid beta (A4) precursor-like protein 1, Amyloid-like protein 1, APLP, APLP-1, APLP1

Recommended products

Rabbit Monoclonal APLP1 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25737-4
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

APLP1 also known as amyloid precursor-like protein 1 is a protein with a molecular mass of approximately 91 kDa. Researchers have observed its expression in the brain particularly in regions like the cortex and hippocampus. This protein shares structural similarities with the Amyloid Precursor Protein (APP) family and plays important roles in cellular functions through its protein domains and motifs.

Biological function summary

APLP1 functions by contributing to cell-cell adhesion and synaptic formation impacting neuronal development. It forms part of a larger protein complex that modulates these processes interacting with other proteins to maintain cellular communication. This protein's activity supports the stability and plasticity of synapses which is essential for proper neural network function.

Pathways

Proteins related to APLP1 participate notably in the Notch signaling and MAPK pathways. These pathways influence cell fate decisions growth and survival. APLP1 interacts with proteins such as APP and APLP2 both of which also engage in similar signaling pathways. This interconnectedness highlights APLP1's role in neurobiological processes essential for normal brain operation.

Associated diseases and disorders

Researchers link APLP1 to neurodegenerative disorders like Alzheimer's disease and autism spectrum disorders. Changes in APLP1 expression or function can affect amyloidogenic processing connecting it to APP and potentially leading to plaque formation a hallmark of Alzheimer's disease. Additionally this protein's relationship with synaptic function may contribute to the pathophysiology observed in autism spectrum disorders where alterations in synaptic communication are common.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097), expandable thumbnail

    Western blot - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097)

    This data was developed using Anti-APLP1 antibody [EPR25737-10] ab291070, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Samples are non-boiled as boiling may cause protein aggregates.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 7494461; PMID: 18430897).
    Low expression: Liver (PMID: 18430897 ).

    All lanes: Western blot - Anti-APLP1 antibody [EPR25737-10] (Anti-APLP1 antibody [EPR25737-10] ab291070) at 1/1000 dilution

    Lanes 1 and 7: Mouse brain tissue lysate 20 µg

    Lane 2: Mouse cerebral cortex tissue lysate 20 µg

    Lane 3: Mouse liver tissue lysate 20 µg

    Lane 4: Rat brain tissue lysate 20 µg

    Lane 5: Rat cerebral cortex tissue lysate 20 µg

    Lane 6: Rat liver tissue lysate 20 µg

    Lane 8: Mouse brain tissue lysate treated with PNGase F 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 72 kDa, 97 kDa, 95 kDa

    Exposure time: 26s

  • Western blot - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097), expandable thumbnail

    Western blot - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097)

    This data was developed using Anti-APLP1 antibody [EPR25737-10] ab291070, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Samples are non-boiled as boiling may cause protein aggregates.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 7494461; PMID: 18430897).
    Low expression: Liver, small intestine, colon cancer (PMID:18430897).

    All lanes: Western blot - Anti-APLP1 antibody [EPR25737-10] (Anti-APLP1 antibody [EPR25737-10] ab291070) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate

    Lane 2: Human liver tissue lysate

    Lane 3: Human small intestine tissue lysate

    Lane 4: Human colon cancer tissue lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 97 kDa

    Exposure time: 26s

  • Flow Cytometry (Intracellular) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097)

    This data was developed using Anti-APLP1 antibody [EPR25737-10] ab291070, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of Mouse primary neuron cell cells labelling APLP1 with Anti-APLP1 antibody [EPR25737-10] ab291070 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097)

    This data was developed using Anti-APLP1 antibody [EPR25737-10] ab291070, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling APLP1 with Anti-APLP1 antibody [EPR25737-10] ab291070 at 1/5000 (0.209 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with Anti-APLP1 antibody [EPR25737-10] ab291070 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097)

    This data was developed using Anti-APLP1 antibody [EPR25737-10] ab291070, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling APLP1 with Anti-APLP1 antibody [EPR25737-10] ab291070 at 1/5000 (0.209 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with Anti-APLP1 antibody [EPR25737-10] ab291070 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097)

    This data was developed using Anti-APLP1 antibody [EPR25737-10] ab291070, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling APLP1 with Anti-APLP1 antibody [EPR25737-10] ab291070 at 1/5000 (0.209 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebrum (PMID: 9444352). The section was incubated with Anti-APLP1 antibody [EPR25737-10] ab291070 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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