AB140624

Anti-APLP2 antibody [EPR5938(2)]

Name: Anti-APLP2 antibody [EPR5938(2)] (ab140624) | Abcam
Brand: Abcam Limited
SKU: AB140624
Price: 335 USD
Availability: InStock
Rating: 2 (1 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal APLP2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

APPL2, APLP2, Amyloid beta precursor like protein 2, APPH, Amyloid beta (A4) precursor-like protein 2, Amyloid protein homolog, Amyloid-like protein 2, CDEI box-binding protein, Sperm membrane protein YWK-II, APLP-2, CDEBP

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] (AB140624)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] (AB140624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling APLP2 with purified ab140624 at 1 : 500 (0.188 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] (AB140624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling APLP2 with purified ab140624 at 1 : 500 (0.188 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as a reporter. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] (AB140624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling APLP2 with purified ab140624 at 1 : 2000 (0.047 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Supplier Data

Western blot - Anti-APLP2 antibody [EPR5938(2)] (AB140624)

The molecular weight observed is consistent with what has been described in the literature (PMID : 16279945).

All lanes:

Western blot - Anti-APLP2 antibody [EPR5938(2)] (ab140624) at 1/10000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 90-120 kDa

false

Carrier free

Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5938(2)

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects isoforms in some cell lines.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "ab140624 detects isoforms in some cell lines", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "We recommend a dilution of 1/500 for human, 1/2000 for rat.For unpurified use at 1/100 - 1/250." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "ab140624 detects isoforms in some cell lines", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "ab140624 detects isoforms in some cell lines", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "We recommend a dilution of 1/500 for human, 1/2000 for rat.For unpurified use at 1/100 - 1/250." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APLP2 also known as Amyloid Precursor-Like Protein 2 is a cell surface protein with a mass of about 92 kDa. This protein belongs to the same family as Amyloid Precursor Protein (APP). APLP2 is notably expressed in many tissues including the brain where its expression is significant. It participates in a variety of cellular functions due to its structural and functional properties similar to that of other amyloid precursor proteins.

Biological function summary

APLP2 participates in the regulation of neuronal growth and synaptic stability. It functions as a part of a larger protein complex and shares structural similarities with other members of its family which suggests overlapping functions. APLP2 interacts with extracellular matrix proteins and may help with cell adhesion processes which are important for maintaining tissue structure and function.

Pathways

The APLP2 protein interacts within signaling pathways that regulate neural development and cell viability. It plays a role in the Notch signaling pathway which is important for cell differentiation processes. Other related proteins such as APP and APLP1 demonstrate similar roles within these pathways indicating a potential coordination among these family members to achieve functional outcomes in tissue development and homeostasis.

APLP2 has been implicated in neurodegenerative diseases such as Alzheimer's disease through its relation to APP. Elevated or misregulated levels of APLP2 have shown connections to increased amyloid-beta production which is a hallmark of Alzheimer's pathology. Furthermore APLP2's involvement in tumorigenesis links it to gliomas with its interaction with proteins like EGFR hinting at its role in cancer-related cellular signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts (By similarity).

See full target information APLP2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

In vivo (Athens, Greece) 38:399-408 PubMed38148084

2023

Implication of Amyloid Precursor-like Protein 2 Expression in Cutaneous Squamous Cell Carcinoma Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodi Huang,Jihye Yang,Haoran Xi,Meilan Zhang,Yeongjoo Oh,Zhehu Jin,Zhenlong Zheng

Environmental toxicology 39:612-625 PubMed37515494

2023

Comprehensive intratumoral heterogeneity landscaping of liver hepatocellular carcinoma and discerning of APLP2 in cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhigang Tao,Jing Huang,Jun Li

Cellular and molecular life sciences : CMLS 73:393-408 PubMed26216398

2015

APP controls the formation of PI(3,5)P(2) vesicles through its binding of the PIKfyve complex.

Applications

Unspecified application

Species

Unspecified reactive species

Heather Currinn,Benjamin Guscott,Zita Balklava,Alice Rothnie,Thomas Wassmer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APLP2 antibody [EPR5938(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] (AB140624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] (AB140624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] (AB140624)

Western blot - Anti-APLP2 antibody [EPR5938(2)] (AB140624)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise