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Rabbit Recombinant Monoclonal APLP2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (AB240117), expandable thumbnail
  • Western blot - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (AB240117), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (AB240117), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (AB240117), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Tested
Tested

Tested
Tested

Species

Rat

Dilution info

-

Notes

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

ab240117 detects isoforms in some cell lines.

Species

Rat

Dilution info

-

Notes

ab240117 detects isoforms in some cell lines.

Species

Human

Dilution info

-

Notes

ab240117 detects isoforms in some cell lines.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts (By similarity).

Alternative names

Amyloid-like protein 2, APLP-2, APPH, Amyloid protein homolog, CDEI box-binding protein, CDEBP, APPL2, APLP2

Recommended products

Rabbit Recombinant Monoclonal APLP2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples.

Alternative names

Amyloid-like protein 2, APLP-2, APPH, Amyloid protein homolog, CDEI box-binding protein, CDEBP, APPL2, APLP2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR5938(2)

Purification technique

Affinity purification Protein A

Specificity

This antibody detects isoforms in some cell lines.
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab240117 is the carrier-free version of Anti-APLP2 antibody [EPR5938(2)] ab140624.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

APLP2 also known as Amyloid Precursor-Like Protein 2 is a cell surface protein with a mass of about 92 kDa. This protein belongs to the same family as Amyloid Precursor Protein (APP). APLP2 is notably expressed in many tissues including the brain where its expression is significant. It participates in a variety of cellular functions due to its structural and functional properties similar to that of other amyloid precursor proteins.

Biological function summary

APLP2 participates in the regulation of neuronal growth and synaptic stability. It functions as a part of a larger protein complex and shares structural similarities with other members of its family which suggests overlapping functions. APLP2 interacts with extracellular matrix proteins and may help with cell adhesion processes which are important for maintaining tissue structure and function.

Pathways

The APLP2 protein interacts within signaling pathways that regulate neural development and cell viability. It plays a role in the Notch signaling pathway which is important for cell differentiation processes. Other related proteins such as APP and APLP1 demonstrate similar roles within these pathways indicating a potential coordination among these family members to achieve functional outcomes in tissue development and homeostasis.

Associated diseases and disorders

APLP2 has been implicated in neurodegenerative diseases such as Alzheimer's disease through its relation to APP. Elevated or misregulated levels of APLP2 have shown connections to increased amyloid-beta production which is a hallmark of Alzheimer's pathology. Furthermore APLP2's involvement in tumorigenesis links it to gliomas with its interaction with proteins like EGFR hinting at its role in cancer-related cellular signaling.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (ab240117), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (ab240117)

    This data was developed using Anti-APLP2 antibody [EPR5938(2)] ab140624, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling APLP2 with purified Anti-APLP2 antibody [EPR5938(2)] ab140624 at 1:500 (0.188 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (ab240117), expandable thumbnail

    Western blot - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (ab240117)

    This data was developed using Anti-APLP2 antibody [EPR5938(2)] ab140624, the same antibody clone in a different buffer formulation. The molecular weight observed is consistent with what has been described in the literature (PMID: 16279945).

    All lanes: Western blot - Anti-APLP2 antibody [EPR5938(2)] (Anti-APLP2 antibody [EPR5938(2)] ab140624) at 1/10000 dilution

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

    Lane 2: Mouse brain lysate at 15 µg

    Lane 3: Rat brain lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa

    Observed band size: 90-120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (ab240117), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (ab240117)

    This data was developed using Anti-APLP2 antibody [EPR5938(2)] ab140624, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling APLP2 with purified Anti-APLP2 antibody [EPR5938(2)] ab140624 at 1:2000 (0.047 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (ab240117), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (ab240117)

    This data was developed using Anti-APLP2 antibody [EPR5938(2)] ab140624, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling APLP2 with purified Anti-APLP2 antibody [EPR5938(2)] ab140624 at 1:500 (0.188 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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