Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (AB240117)

This data was developed using ab140624, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling APLP2 with purified ab140624 at 1 : 500 (0.188 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (AB240117)

This data was developed using ab140624, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling APLP2 with purified ab140624 at 1 : 500 (0.188 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (AB240117)

This data was developed using ab140624, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling APLP2 with purified ab140624 at 1 : 2000 (0.047 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Supplier Data

Western blot - Anti-APLP2 antibody [EPR5938(2)] - BSA and Azide free (AB240117)

This data was developed using ab140624, the same antibody clone in a different buffer formulation. The molecular weight observed is consistent with what has been described in the literature (PMID : 16279945).

All lanes:

Western blot - Anti-APLP2 antibody [EPR5938(2)] (<a href='/en-us/products/primary-antibodies/aplp2-antibody-epr59382-ab140624'>ab140624</a>) at 1/10000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 90-120 kDa

false