AB256496

Anti-Apo-D antibody [EPR22967-72]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal Apo-D antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Apolipoprotein D, Apo-D, ApoD, APOD

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (AB256496)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID : 18330697, 18505051). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of human colon (PMID : 23296401). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Unknown

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Apo-D was immunoprecipitated from 0.35 mg Human plasma lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human plasma lysate 10ug

Lane 2 : ab256496 IP in Human plasma lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human plasma lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

Predicted band size: 21 kDa

false

Unknown

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Apo-D was immunoprecipitated from 0.35 mg Human placenta lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human placenta lysate 10ug

Lane 2 : ab256496 IP in Human placenta lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human placenta lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

Predicted band size: 21 kDa

false

Lab

Western blot - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The smeared bands between 22-35 kDa are mainly caused by different levels of glycosylation (PMID : 26829325).

Exposure time : Lane 1 : 26 seconds; Lanes 2-3 : 3 minutes.

All lanes:

Western blot - Anti-Apo-D antibody [EPR22967-72] (ab256496) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 22-35 kDa

false

Carrier free

Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22967-72

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apo-D also known as apolipoprotein D is a glycoprotein with an approximate mass of 29 kilodaltons. As an apolipoprotein Apo-D is part of the lipocalin family which participates in the transport of small hydrophobic molecules. It is widely expressed in human tissues particularly in the brain liver and adrenal glands. The expression of Apo-D in these tissues suggests important roles in diverse physiological processes and lipid metabolism. Scientists often study Apo-D to understand its involvement in lipid transport and its biological activities.

Biological function summary

Apo-D interacts with various molecules affecting lipid and cholesterol transport and metabolism. Apo-D operates as part of a protein complex and binds ligands such as progesterone and arachidonic acid. These interactions suggest it might modulate their availability and activity in biological systems. Furthermore the role of Apo-D in protecting cells against oxidative stress emerges as an important mechanism of action contributing to its protective properties in neural and other tissues.

Pathways

Apo-D plays significant roles by influencing the HDL (high-density lipoprotein) metabolism pathway and the arachidonic acid pathway. Within the HDL pathway Apo-D associates with other apolipoproteins like apoA-I to regulate cholesterol transport and anti-inflammatory responses. Its involvement in the arachidonic acid pathway suggests a possible regulatory effect on inflammation and cellular stress responses highlighting the complex interaction of Apo-D with other proteins like cyclooxygenases which are key players in inflammation.

Researchers have linked Apo-D to conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's disease its altered expression in the brain suggests a protective response or involvement in disease progression possibly through interaction with amyloid precursor proteins or influencing cholesterol metabolism. In schizophrenia abnormal levels of Apo-D in cerebrospinal fluid mark potential disturbance in lipid metabolism making it a protein of interest in the disease's complex pathophysiology. Understanding these connections provides insight into therapeutic strategies that target the biological functions of Apo-D.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

See full target information Apolipoprotein D

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102024 PubMed40107247

2025

Immune-featured stromal niches associate with response to neoadjuvant immunotherapy in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Tong Liu,Hai-Ming Liu,Jian-Gang Ren,Wei Zhang,Xin-Xin Wang,Zi-Li Yu,Qiu-Yun Fu,Xue-Peng Xiong,Jun Jia,Bing Liu,Gang Chen

Cell proliferation 58:e13818 PubMed39902627

2025

New Anti-Fibrotic Strategies for Keloids: Insights From Single-Cell Multi-Omics.

Applications

Unspecified application

Species

Unspecified reactive species

Songyun Zhao,Jiaheng Xie,Qian Zhang,Tianyi Ni,Jinde Lin,Weicheng Gao,Liping Zhao,Min Yi,Liying Tu,Pengpeng Zhang,Dan Wu,Qikai Tang,Chenfeng Ma,Yucang He,Liqun Li,Guoping Wu,Wei Yan

Biomedicines 12: PubMed39767614

2025

Precision Treatment of Metachronous Multiple Primary Malignancies Based on Constructing Patient Tumor-Derived Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Yicheng Wang,Haotian Chen,Zhijin Zhang,Yanyan He,Ji Liu,Baoshuang Zhao,Qinwan Wang,Jiangmei Xu,Shiyu Mao,Wentao Zhang,Xudong Yao,Wei Li

Cell reports. Medicine 4:101277 PubMed37944531

2023

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxue Ren,Mingle Huang,Weixiang Weng,Yubin Xie,Yifan Wu,Shenghua Zhu,Ying Zhang,Dongming Li,Jiaming Lai,Shunli Shen,Jie Lin,Ming Kuang,Xiaoxing Li,Jun Yu,Lixia Xu

Frontiers in genetics 13:686739 PubMed35601482

2022

Single-Cell Transcriptomics Uncovers Cellular Heterogeneity, Mechanisms, and Therapeutic Targets for Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjun Huang,Li Liu,Lingling Qin,Hehua Huang,Xue Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apo-D antibody [EPR22967-72]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Western blot - Anti-Apo-D antibody [EPR22967-72] (AB256496)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise