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Rabbit Recombinant Monoclonal Apo-D antibody. Suitable for IP, WB, IHC-P and reacts with Human samples.

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Images

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (AB256496), expandable thumbnail
  • Western blot - Anti-Apo-D antibody [EPR22967-72] (AB256496), expandable thumbnail
  • Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (AB256496), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (AB256496), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (AB256496), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/40
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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3 products for Alternative Product

Target data

Function

APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

Alternative names

Apolipoprotein D, Apo-D, ApoD, APOD

Recommended products

Rabbit Recombinant Monoclonal Apo-D antibody. Suitable for IP, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22967-72
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Apo-D also known as apolipoprotein D is a glycoprotein with an approximate mass of 29 kilodaltons. As an apolipoprotein Apo-D is part of the lipocalin family which participates in the transport of small hydrophobic molecules. It is widely expressed in human tissues particularly in the brain liver and adrenal glands. The expression of Apo-D in these tissues suggests important roles in diverse physiological processes and lipid metabolism. Scientists often study Apo-D to understand its involvement in lipid transport and its biological activities.

Biological function summary

Apo-D interacts with various molecules affecting lipid and cholesterol transport and metabolism. Apo-D operates as part of a protein complex and binds ligands such as progesterone and arachidonic acid. These interactions suggest it might modulate their availability and activity in biological systems. Furthermore the role of Apo-D in protecting cells against oxidative stress emerges as an important mechanism of action contributing to its protective properties in neural and other tissues.

Pathways

Apo-D plays significant roles by influencing the HDL (high-density lipoprotein) metabolism pathway and the arachidonic acid pathway. Within the HDL pathway Apo-D associates with other apolipoproteins like apoA-I to regulate cholesterol transport and anti-inflammatory responses. Its involvement in the arachidonic acid pathway suggests a possible regulatory effect on inflammation and cellular stress responses highlighting the complex interaction of Apo-D with other proteins like cyclooxygenases which are key players in inflammation.

Associated diseases and disorders

Researchers have linked Apo-D to conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's disease its altered expression in the brain suggests a protective response or involvement in disease progression possibly through interaction with amyloid precursor proteins or influencing cholesterol metabolism. In schizophrenia abnormal levels of Apo-D in cerebrospinal fluid mark potential disturbance in lipid metabolism making it a protein of interest in the disease's complex pathophysiology. Understanding these connections provides insight into therapeutic strategies that target the biological functions of Apo-D.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496), expandable thumbnail

    Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Apo-D was immunoprecipitated from 0.35 mg Human placenta lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human placenta lysate 10ug

    Lane 2: ab256496 IP in Human placenta lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab256496 in Human placenta lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Predicted band size: 21 kDa

  • Western blot - Anti-Apo-D antibody [EPR22967-72] (ab256496), expandable thumbnail

    Western blot - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The smeared bands between 22-35 kDa are mainly caused by different levels of glycosylation (PMID: 26829325).

    Exposure time: Lane 1: 26 seconds; Lanes 2-3: 3 minutes.

    All lanes: Western blot - Anti-Apo-D antibody [EPR22967-72] (ab256496) at 1/1000 dilution

    Lane 1: Human plasma at 20 µg

    Lane 2: Human heart tissue lysate at 20 µg

    Lane 3: Human kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 21 kDa

    Observed band size: 22-35 kDa

  • Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496), expandable thumbnail

    Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Apo-D was immunoprecipitated from 0.35 mg Human plasma lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human plasma lysate 10ug

    Lane 2: ab256496 IP in Human plasma lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab256496 in Human plasma lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID:18330697, 18505051). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of human colon (PMID: 23296401). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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