Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (AB261911)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID : 18330697, 18505051). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256496).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (AB261911)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of human colon (PMID : 23296401). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256496).

• IP

Unknown

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (AB261911)

Apo-D was immunoprecipitated from 0.35 mg Human plasma lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human plasma lysate 10ug

Lane 2 : ab256496 IP in Human plasma lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human plasma lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256496).

All lanes:

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (<a href='/en-us/products/primary-antibodies/apo-d-antibody-epr22967-72-ab256496'>ab256496</a>)

Predicted band size: 21 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (AB261911)

Apo-D was immunoprecipitated from 0.35 mg Human placenta lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human placenta lysate 10ug

Lane 2 : ab256496 IP in Human placenta lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human placenta lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256496).

All lanes:

Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (<a href='/en-us/products/primary-antibodies/apo-d-antibody-epr22967-72-ab256496'>ab256496</a>)

Predicted band size: 21 kDa

false