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AB108191

Anti-Apo-D antibody [EPR2916]

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(8 Publications)

Rabbit Recombinant Monoclonal Apo-D antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

Apolipoprotein D, Apo-D, ApoD, APOD

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (AB108191)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (AB108191)

Immunohistochemical staining of Apo-D in paraffin embedded Human liver tissue using unpurified ab108191 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (AB108191)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (AB108191)

Immunohistochemical staining of Apo-D in paraffin embedded human kidney tissue using unpurified ab108191 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (AB108191)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (AB108191)

Immunohistochemical staining of paraffin embedded human liver with purified ab108191 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Western blot - Anti-Apo-D antibody [EPR2916] (AB108191)
  • WB

Unknown

Western blot - Anti-Apo-D antibody [EPR2916] (AB108191)

All lanes:

Western blot - Anti-Apo-D antibody [EPR2916] (ab108191) at 1/1000 dilution

All lanes:

Human plasma cell lysate at 10 µg

Predicted band size: 21 kDa

false

Western blot - Anti-Apo-D antibody [EPR2916] (AB108191)
  • WB

Lab

Western blot - Anti-Apo-D antibody [EPR2916] (AB108191)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Apo-D antibody [EPR2916] (ab108191) at 1/2000 dilution

All lanes:

human plasma at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 21 kDa,86 kDa

Observed band size: 100 kDa,30 kDa

false

  • Carrier free

    Anti-Apo-D antibody [EPR2916] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2916

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apo-D also known as apolipoprotein D is a glycoprotein with an approximate mass of 29 kilodaltons. As an apolipoprotein Apo-D is part of the lipocalin family which participates in the transport of small hydrophobic molecules. It is widely expressed in human tissues particularly in the brain liver and adrenal glands. The expression of Apo-D in these tissues suggests important roles in diverse physiological processes and lipid metabolism. Scientists often study Apo-D to understand its involvement in lipid transport and its biological activities.
Biological function summary

Apo-D interacts with various molecules affecting lipid and cholesterol transport and metabolism. Apo-D operates as part of a protein complex and binds ligands such as progesterone and arachidonic acid. These interactions suggest it might modulate their availability and activity in biological systems. Furthermore the role of Apo-D in protecting cells against oxidative stress emerges as an important mechanism of action contributing to its protective properties in neural and other tissues.

Pathways

Apo-D plays significant roles by influencing the HDL (high-density lipoprotein) metabolism pathway and the arachidonic acid pathway. Within the HDL pathway Apo-D associates with other apolipoproteins like apoA-I to regulate cholesterol transport and anti-inflammatory responses. Its involvement in the arachidonic acid pathway suggests a possible regulatory effect on inflammation and cellular stress responses highlighting the complex interaction of Apo-D with other proteins like cyclooxygenases which are key players in inflammation.

Researchers have linked Apo-D to conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's disease its altered expression in the brain suggests a protective response or involvement in disease progression possibly through interaction with amyloid precursor proteins or influencing cholesterol metabolism. In schizophrenia abnormal levels of Apo-D in cerebrospinal fluid mark potential disturbance in lipid metabolism making it a protein of interest in the disease's complex pathophysiology. Understanding these connections provides insight into therapeutic strategies that target the biological functions of Apo-D.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
See full target information Apolipoprotein D
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 28:515 PubMed39247492

2024

Analysis of metastasis‑related risk factors and clinical relevance in adult soft‑tissue sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Han,Xin Song,Jialiang Liu,Jingfen Zhou,Zhipeng Wu,Haihan Song,Jun Tao,Jian Wang

NPJ Parkinson's disease 10:143 PubMed39095480

2024

Astrocyte-derived apolipoprotein D is required for neuronal survival in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Dai,Mingxia Bi,Qian Jiao,Xixun Du,Chunling Yan,Hong Jiang

European journal of histochemistry : EJH 67: PubMed36546419

2023

Differential antigen expression between human apocrine sweat glands and eccrine sweat glands.

Applications

Unspecified application

Species

Unspecified reactive species

Manxiu Cao,Lei Zhang,Jiaqi Chen,Cangyu Wang,Junhong Zhao,Xiang Liu,Yongjing Yan,Yue Tang,Zixiu Chen,Haihong Li

Disease markers 2021:4251763 PubMed34804261

2021

Development and Verification of an Immune-Based Gene Signature for Risk Stratification and Immunotherapeutic Efficacy Assessment in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Qiu,Yumei Zhu,Yafeng Shi,Jingjing Ji,Yingchao Jin

Cell discovery 7:36 PubMed34035226

2021

Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Yiyi Liang,Haiyan Xu,Xiao Zhang,Tiebo Mao,Jiujie Cui,Jiayu Yao,Yongchao Wang,Feng Jiao,Xiuying Xiao,Jiong Hu,Qing Xia,Xiaofei Zhang,Xujun Wang,Yongwei Sun,Deliang Fu,Lei Shen,Xiaojiang Xu,Jing Xue,Liwei Wang

International journal of molecular sciences 18: PubMed28212277

2017

Clinical and Molecular Evidence of ABCC11 Protein Expression in Axillary Apocrine Glands of Patients with Axillary Osmidrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Toyoda,Tappei Takada,Tsuneaki Gomi,Hiroshi Nakagawa,Toshihisa Ishikawa,Hiroshi Suzuki

Arkhiv patologii 78:9-14 PubMed27804940

2016

[Estimation of the diagnostic potential of APOD, PTOV1, and EPHA4 for prostatic neoplasms].

Applications

Unspecified application

Species

Unspecified reactive species

D O Allina,Yu Yu Andreeva,L E Zavalishina,L V Moskvina,G A Frank

Neurobiology of aging 36:1820-33 PubMed25784209

2015

Apolipoprotein D modulates amyloid pathology in APP/PS1 Alzheimer's disease mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Kalani Ruberu,Sonia Sanz Muñoz,Andrew M Jenner,Adena Spiro,Hua Zhao,Eric Rassart,Diego Sanchez,Maria D Ganfornina,Tim Karl,Brett Garner
View all publications
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Product promise

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