Rabbit Polyclonal Apo-H antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human APOH aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human APOH aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P02749
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
Alternative names
B2G1, APOH, Beta-2-glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Apolipoprotein H, Beta-2-glycoprotein I, Apo-H, B2GPI, Beta(2)GPI
Recommended products
Rabbit Polyclonal Apo-H antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human APOH aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human APOH aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P02749
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein H (Apo-H) also known as β2-glycoprotein I functions mechanically as a plasma protein. It has a molecular weight of approximately 50 kDa and it is mostly expressed in the liver. Apo-H plays a role in the body's immune and coagulation systems binding to anionic surfaces such as phospholipids and preventing blood coagulation. Its ability to interact with negatively charged molecules makes it important in the modulation of the coagulation cascade helping maintain balance between bleeding and clotting.
- Biological function summary
Apo-H acts as an anticoagulant and participates in the modulation of immune response. It forms complexes with lipid-bound proteins facilitating the clearance of these complexes from the bloodstream. This interaction is key in processes like platelet aggregation and stabilization of membranes. Apo-H also influences the removal of oxidized proteins and lipids reducing cellular stress and preventing tissue damage.
- Pathways
Apo-H plays a critical role in the coagulation cascade and lipid metabolism. It interacts with proteins such as prothrombin and fibrinogen influencing blood coagulation pathways. Apo-H's involvement in the clearance of apoptotic cells integrates into the lipid metabolic pathway highlighting its relationship with apolipoproteins and other lipid-related proteins. These interactions illustrate the multi-functional aspects of Apo-H within these pathways.
- Associated diseases and disorders
Apo-H associates with antiphospholipid syndrome and cardiovascular diseases. These conditions arise from its role in coagulation and immune response where disruptions can lead to increased risk of thrombosis. The protein's interaction with other apolipoproteins such as Apo-A1 highlights possible implications in lipid metabolism disorders which in turn can exacerbate cardiovascular risk. Understanding these associations provides insights into potential therapeutic targets for treating these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-H antibody (ab236960)
Paraffin-embedded human liver cancer tissue stained for Apo-H using ab236960 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Apo-H antibody (ab236960)
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Apo-H (green) using ab236960 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com