AB247640

Anti-Apo-H antibody [EPR2898(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Apo-H antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

B2G1, APOH, Beta-2-glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Apolipoprotein H, Beta-2-glycoprotein I, Apo-H, B2GPI, Beta(2)GPI

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Apo-H antibody [EPR2898(2)] - BSA and Azide free (AB247640)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Apo-H antibody [EPR2898(2)] - BSA and Azide free (AB247640)

This data was developed using ab108348, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HepG2 cells using ab108348 at a dilution of 1/100.

Unknown

Western blot - Anti-Apo-H antibody [EPR2898(2)] - BSA and Azide free (AB247640)

This data was developed using ab108348, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Apo-H antibody [EPR2898(2)] (<a href='/en-us/products/primary-antibodies/apo-h-antibody-epr28982-ab108348'>ab108348</a>) at 1/10000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

HDL lysate at 10 µg

Predicted band size: 38 kDa

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Lab

Western blot - Anti-Apo-H antibody [EPR2898(2)] - BSA and Azide free (AB247640)

This data was developed using ab108348, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot analysis, ab108348 exhibited lower sensitivity compared to ab267464, for which reason we recommend ab108348 as superior alternative.

Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1 : 500), and fg-grade ECL substrates are suggested if ab267464 must be used.

Lane 1:

Western blot - Anti-Apo-H antibody [EPR2898(2)] (<a href='/en-us/products/primary-antibodies/apo-h-antibody-epr28982-ab108348'>ab108348</a>) at 1/1000 dilution

Lane 2:

Western blot - Anti-Apo-H antibody [EPR23087-228] (<a href='/en-us/products/primary-antibodies/apo-h-antibody-epr23087-228-ab267464'>ab267464</a>) at 1/1000 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50 kDa,60 kDa

false

Exposure time: 60s

Unconjugated

Anti-Apo-H antibody [EPR2898(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2898(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "In Western blot analysis, <a href='/en-us/products/primary-antibodies/apo-h-antibody-epr28982-ab108348'>ab108348</a> exhibited lower sensitivity compared to <a href='/en-us/products/primary-antibodies/apo-h-antibody-epr23087-228-ab267464'>ab267464</a>, for which reason we recommend <a href='/en-us/products/primary-antibodies/apo-h-antibody-epr28982-ab108348'>ab108348</a> as superior alternative.Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if <a href='/en-us/products/primary-antibodies/apo-h-antibody-epr23087-228-ab267464'>ab267464</a> must be used.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab247640 is the carrier-free version of ab108348.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein H (Apo-H) also known as β2-glycoprotein I functions mechanically as a plasma protein. It has a molecular weight of approximately 50 kDa and it is mostly expressed in the liver. Apo-H plays a role in the body's immune and coagulation systems binding to anionic surfaces such as phospholipids and preventing blood coagulation. Its ability to interact with negatively charged molecules makes it important in the modulation of the coagulation cascade helping maintain balance between bleeding and clotting.

Biological function summary

Apo-H acts as an anticoagulant and participates in the modulation of immune response. It forms complexes with lipid-bound proteins facilitating the clearance of these complexes from the bloodstream. This interaction is key in processes like platelet aggregation and stabilization of membranes. Apo-H also influences the removal of oxidized proteins and lipids reducing cellular stress and preventing tissue damage.

Pathways

Apo-H plays a critical role in the coagulation cascade and lipid metabolism. It interacts with proteins such as prothrombin and fibrinogen influencing blood coagulation pathways. Apo-H's involvement in the clearance of apoptotic cells integrates into the lipid metabolic pathway highlighting its relationship with apolipoproteins and other lipid-related proteins. These interactions illustrate the multi-functional aspects of Apo-H within these pathways.

Apo-H associates with antiphospholipid syndrome and cardiovascular diseases. These conditions arise from its role in coagulation and immune response where disruptions can lead to increased risk of thrombosis. The protein's interaction with other apolipoproteins such as Apo-A1 highlights possible implications in lipid metabolism disorders which in turn can exacerbate cardiovascular risk. Understanding these associations provides insights into potential therapeutic targets for treating these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

See full target information APOH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 12:161 PubMed29967576

2018

Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Mages,Susanne Aleithe,Stephan Altmann,Alexandra Blietz,Björn Nitzsche,Henryk Barthel,Anja K E Horn,Constance Hobusch,Wolfgang Härtig,Martin Krueger,Dominik Michalski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apo-H antibody [EPR2898(2)] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Apo-H antibody [EPR2898(2)] - BSA and Azide free (AB247640)

Western blot - Anti-Apo-H antibody [EPR2898(2)] - BSA and Azide free (AB247640)

Western blot - Anti-Apo-H antibody [EPR2898(2)] - BSA and Azide free (AB247640)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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