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AB303681

Anti-Apo-M antibody [EPR25739-112] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Apo-M antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

G3A, NG20, HSPC336, APOM, Apolipoprotein M, Apo-M, ApoM, Protein G3a

1 Images
Sandwich ELISA - Anti-Apo-M antibody [EPR25739-112] - BSA and Azide free (Detector) (AB303681)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Apo-M antibody [EPR25739-112] - BSA and Azide free (Detector) (AB303681)

Sandwich ELISA of ab303679 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25739-112

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab303681 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human Apo-M.

The recommended pair for sandwich ELISA is:

Capture: ab303680, human Apo-M Capture Antibody (unconjugated)

Detector: ab303681, human Apo-M Detector Antibody (unconjugated)

The reference range value is 125-4000pg/ml

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein M (Apo-M) also known as protein G3a is a 25 kDa member of the lipocalin family which is secreted primarily by liver and kidney cells. Apo-M is a component of high-density lipoprotein (HDL) where it plays a significant role in lipid metabolism. It serves as a binding partner for lipophilic molecules such as sphingosine-1-phosphate (S1P) impacting the stability and functionality of HDL particles. Researchers use anti-Apo antibodies to investigate the expression localization and function of Apo-M in various conditions.
Biological function summary

Apo-M influences several critical processes. It binds with S1P which is involved in endothelial barrier function vascular development and immune cell trafficking. Apo-M is also important for maintaining the inverse relationship between cholesterol levels and cardiovascular risk. It functions within HDL complexes affecting cholesterol efflux and anti-inflammatory responses. Apart from HDL Apo-M interacts with other Apo proteins like apoA-I and apoA-II serving as a regulatory component within these lipoprotein complexes.

Pathways

Apo-M integrates into lipid metabolism and signal transduction pathways. It participates in the S1P signaling pathway which is important for maintaining vascular integrity and immune function. Apo-M's interactions within this pathway influence the activity of other proteins such as the S1P receptors which play a critical role in mediating its biological activities. Additionally Apo-M interfaces with cholesterol efflux pathways interacting with the ATP-binding cassette transporters particularly ABCA1 that facilitate cholesterol removal from peripheral tissues.

Apo-M has associations with atherosclerosis and metabolic syndrome. Dysregulation of Apo-M levels can contribute to the development of atherosclerotic plaques as it affects HDL's protective roles against oxidation and inflammation. Alterations in Apo-M levels may also influence metabolic syndrome components given its function in lipid metabolism and glucose homeostasis. In these contexts changes in Apo-M can affect proteins like apoE which are involved in lipid transport and cardiovascular risk modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
See full target information APOM
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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