AB91656

Anti-Apo-M antibody [EPR2904]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal Apo-M antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

G3A, NG20, HSPC336, APOM, Apolipoprotein M, Apo-M, ApoM, Protein G3a

1 Images

Unknown

Western blot - Anti-Apo-M antibody [EPR2904] (AB91656)

The molecular weights observed are consistent with what have been described in the literature (PMID : 15102887, 16682745, 10531326)

All lanes:

Western blot - Anti-Apo-M antibody [EPR2904] (ab91656) at 1/2000 dilution

Lane 1:

Human plasma lysate at 20 µg

Lane 2:

Mouse plasma lysate at 20 µg

Lane 3:

Rat plasma lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 21 kDa

Observed band size: 18 kDa,23 kDa

false

Carrier free

Anti-Apo-M antibody [EPR2904] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2904

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/2000 - 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/2000 - 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/2000 - 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein M (Apo-M) also known as protein G3a is a 25 kDa member of the lipocalin family which is secreted primarily by liver and kidney cells. Apo-M is a component of high-density lipoprotein (HDL) where it plays a significant role in lipid metabolism. It serves as a binding partner for lipophilic molecules such as sphingosine-1-phosphate (S1P) impacting the stability and functionality of HDL particles. Researchers use anti-Apo antibodies to investigate the expression localization and function of Apo-M in various conditions.

Biological function summary

Apo-M influences several critical processes. It binds with S1P which is involved in endothelial barrier function vascular development and immune cell trafficking. Apo-M is also important for maintaining the inverse relationship between cholesterol levels and cardiovascular risk. It functions within HDL complexes affecting cholesterol efflux and anti-inflammatory responses. Apart from HDL Apo-M interacts with other Apo proteins like apoA-I and apoA-II serving as a regulatory component within these lipoprotein complexes.

Pathways

Apo-M integrates into lipid metabolism and signal transduction pathways. It participates in the S1P signaling pathway which is important for maintaining vascular integrity and immune function. Apo-M's interactions within this pathway influence the activity of other proteins such as the S1P receptors which play a critical role in mediating its biological activities. Additionally Apo-M interfaces with cholesterol efflux pathways interacting with the ATP-binding cassette transporters particularly ABCA1 that facilitate cholesterol removal from peripheral tissues.

Apo-M has associations with atherosclerosis and metabolic syndrome. Dysregulation of Apo-M levels can contribute to the development of atherosclerotic plaques as it affects HDL's protective roles against oxidation and inflammation. Alterations in Apo-M levels may also influence metabolic syndrome components given its function in lipid metabolism and glucose homeostasis. In these contexts changes in Apo-M can affect proteins like apoE which are involved in lipid transport and cardiovascular risk modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.

See full target information APOM

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Angiogenesis 28:24 PubMed40266369

2025

ApoM-bound S1P acts via endothelial S1PR1 to suppress choroidal neovascularization and vascular leakage.

Applications

Unspecified application

Species

Unspecified reactive species

Bongnam Jung,Hitomi Yagi,Andrew Kuo,Tim F Dorweiler,Masanori Aikawa,Taku Kasai,Sasha A Singh,Andrew J Dannenberg,Zhongjie Fu,Colin Niaudet,Lois E H Smith,Timothy Hla

Science signaling 17:eadg9256 PubMed38377179

2024

Designer high-density lipoprotein particles enhance endothelial barrier function and suppress inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yueh-Chien Lin,Steven Swendeman,Irina S Moreira,Avishek Ghosh,Andrew Kuo,Nícia Rosário-Ferreira,Shihui Guo,Alan Culbertson,Michel V Levesque,Andreane Cartier,Takahiro Seno,Alec Schmaier,Sylvain Galvani,Asuka Inoue,Samir M Parikh,Garret A FitzGerald,David Zurakowski,Maofu Liao,Robert Flaumenhaft,Zeynep H Gümüş,Timothy Hla

eLife 11: PubMed36197001

2022

Murine endothelial serine palmitoyltransferase 1 (SPTLC1) is required for vascular development and systemic sphingolipid homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Kuo,Antonio Checa,Colin Niaudet,Bongnam Jung,Zhongjie Fu,Craig E Wheelock,Sasha A Singh,Masanori Aikawa,Lois E Smith,Richard L Proia,Timothy Hla

Biochimica et biophysica acta. Molecular and cell 1865:158632 PubMed31954174

2020

Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Del Gaudio,Ivana Sreckovic,Pablo Zardoya-Laguardia,Eva Bernhart,Christina Christoffersen,Saša Frank,Gunther Marsche,Sebastian E Illanes,Christian Wadsack

Journal of lipid research 60:1912-1921 PubMed31462513

2019

Identification of ApoA4 as a sphingosine 1-phosphate chaperone in ApoM- and albumin-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hideru Obinata,Andrew Kuo,Yukata Wada,Steven Swendeman,Catherine H Liu,Victoria A Blaho,Rieko Nagumo,Kenichi Satoh,Takashi Izumi,Timothy Hla

Arthritis & rheumatology (Hoboken, N.J.) 70:1879-1889 PubMed29781582

2018

Sphingosine 1-Phosphate Receptor 1 Signaling Maintains Endothelial Cell Barrier Function and Protects Against Immune Complex-Induced Vascular Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Burg,Steven Swendeman,Stefan Worgall,Timothy Hla,Jane E Salmon

Journal of lipid research 56:754-9 PubMed25561460

2015

Protein unfolding allows use of commercial antibodies in an apolipoprotein M sandwich ELISA.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Høybye Bosteen,Björn Dahlbäck,Lars Bo Nielsen,Christina Christoffersen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apo-M antibody [EPR2904]

Western blot - Anti-Apo-M antibody [EPR2904] (AB91656)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise