AB239902

Anti-Apo-M antibody [EPR2904] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Apo-M antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

G3A, NG20, HSPC336, APOM, Apolipoprotein M, Apo-M, ApoM, Protein G3a

1 Images

Unknown

Western blot - Anti-Apo-M antibody [EPR2904] - BSA and Azide free (AB239902)

The molecular weights observed are consistent with what have been described in the literature (PMID : 15102887, 16682745, 10531326).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91656).

All lanes:

Western blot - Anti-Apo-M antibody [EPR2904] (<a href='/en-us/products/primary-antibodies/apo-m-antibody-epr2904-ab91656'>ab91656</a>) at 1/2000 dilution

Lane 1:

Human plasma lysate at 20 µg

Lane 2:

Mouse plasma lysate at 20 µg

Lane 3:

Rat plasma lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 21 kDa

Observed band size: 18 kDa,23 kDa

false

Unconjugated

Anti-Apo-M antibody [EPR2904]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2904

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab239902 is the carrier-free version of ab91656.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein M (Apo-M) also known as protein G3a is a 25 kDa member of the lipocalin family which is secreted primarily by liver and kidney cells. Apo-M is a component of high-density lipoprotein (HDL) where it plays a significant role in lipid metabolism. It serves as a binding partner for lipophilic molecules such as sphingosine-1-phosphate (S1P) impacting the stability and functionality of HDL particles. Researchers use anti-Apo antibodies to investigate the expression localization and function of Apo-M in various conditions.

Biological function summary

Apo-M influences several critical processes. It binds with S1P which is involved in endothelial barrier function vascular development and immune cell trafficking. Apo-M is also important for maintaining the inverse relationship between cholesterol levels and cardiovascular risk. It functions within HDL complexes affecting cholesterol efflux and anti-inflammatory responses. Apart from HDL Apo-M interacts with other Apo proteins like apoA-I and apoA-II serving as a regulatory component within these lipoprotein complexes.

Pathways

Apo-M integrates into lipid metabolism and signal transduction pathways. It participates in the S1P signaling pathway which is important for maintaining vascular integrity and immune function. Apo-M's interactions within this pathway influence the activity of other proteins such as the S1P receptors which play a critical role in mediating its biological activities. Additionally Apo-M interfaces with cholesterol efflux pathways interacting with the ATP-binding cassette transporters particularly ABCA1 that facilitate cholesterol removal from peripheral tissues.

Apo-M has associations with atherosclerosis and metabolic syndrome. Dysregulation of Apo-M levels can contribute to the development of atherosclerotic plaques as it affects HDL's protective roles against oxidation and inflammation. Alterations in Apo-M levels may also influence metabolic syndrome components given its function in lipid metabolism and glucose homeostasis. In these contexts changes in Apo-M can affect proteins like apoE which are involved in lipid transport and cardiovascular risk modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.

See full target information APOM

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communications 631:138-145 PubMed36183555

2022

SARS-CoV-2-induced hypomethylation of the ferritin heavy chain (FTH1) gene underlies serum hyperferritinemia in severe COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jibran Sualeh Muhammad,Gehad ElGhazali,Jasmin Shafarin,Mohammad G Mohammad,Ameera Abu-Qiyas,Mawieh Hamad

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apo-M antibody [EPR2904] - BSA and Azide free

Western blot - Anti-Apo-M antibody [EPR2904] - BSA and Azide free (AB239902)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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