AB300086

Anti-APOA1 antibody [EPR25446-9] (BSA and Azide free)

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal Apolipoprotein A I antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

View Alternative Names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, Apoa1

2 Images

Supplier Data

Western blot - Anti-APOA1 antibody [EPR25446-9] (BSA and Azide free) (AB300086)

This data was developed using ab300085, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration were 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 23969698, 23409054).
Low expression : brain (PMID : 1478650)

All lanes:

Western blot - Anti-APOA1 antibody [EPR25446-9] (<a href='/en-us/products/primary-antibodies/apoa1-antibody-epr25446-9-ab300085'>ab300085</a>) at 1/1000 dilution

Lane 1:

Mouse plasma at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 30 kDa

Observed band size: 28 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-APOA1 antibody [EPR25446-9] (BSA and Azide free) (AB300086)

This data was developed using ab300085, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200,000 dilution.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Apoa1-KO homozygous mice (Strain ID : T013812).

All lanes:

Western blot - Anti-APOA1 antibody [EPR25446-9] (<a href='/en-us/products/primary-antibodies/apoa1-antibody-epr25446-9-ab300085'>ab300085</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse liver tissue lysate (female) at 20 µg

Lane 2:

Apoa1 knockout mouse liver tissue lysate (female) at 20 µg

Lane 3:

Wild-type mouse liver tissue lysate (male) at 20 µg

Lane 4:

Apoa1 knockout mouse liver tissue lysate (male) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 28 kDa,36 kDa

false

Exposure time: 10s

Unconjugated

Anti-APOA1 antibody [EPR25446-9]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25446-9

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOA1 also known as Apolipoprotein A-I plays an important role in lipid metabolism. Mechanically APOA1 acts as the major protein component of high-density lipoprotein (HDL) in plasma. It facilitates cholesterol efflux from tissues to the liver for excretion. With a molecular weight of approximately 28 kDa APOA1 is expressed mainly in the liver and small intestine. This protein is also involved in lecithin-cholesterol acyltransferase (LCAT) activation which is important for the maturation of HDL particles.

Biological function summary

APOA1 is instrumental in reverse cholesterol transport a process that removes excess cholesterol from cells and prevents lipid accumulation. APOA1 is part of the HDL complex and it directly interacts with various lipid molecules. It contributes to lipid homeostasis and has antioxidant properties reducing oxidative stress. APOA1 also has anti-inflammatory effects which play a role in maintaining cardiovascular health.

Pathways

The reverse cholesterol transport pathway is highly reliant on APOA1 functionality supporting cholesterol movement out of cells. In this pathway APOA1 interacts with ATP-binding cassette transporters like ABCA1 and ABCG1 which aid in transferring cholesterol to HDL particles. Additionally APOA1 is associated with the HDL metabolism pathway where it collaborates with other proteins such as Apolipoprotein A-II (APOA2) and Lecithin-Cholesterol Acyltransferase (LCAT) facilitating the conversion of nascent HDL into mature HDL.

APOA1 has significant implications in cardiovascular diseases and dyslipidemia. Deficiency or dysfunction of APOA1 can lead to lowered HDL levels which correlates with an increased risk of atherosclerosis and coronary artery disease. Furthermore research shows a connection between APOA1 and amyloid-related diseases; abnormal processing or accumulation of apolipoprotein A-I can contribute to amyloidosis. The interactions with proteins like Apolipoprotein E (APOE) are noted as APOE plays a role in lipid metabolism and is implicated in neurodegenerative disorders such as Alzheimer's disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

See full target information Apoa1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APOA1 antibody [EPR25446-9] (BSA and Azide free)

Western blot - Anti-APOA1 antibody [EPR25446-9] (BSA and Azide free) (AB300086)

Western blot - Anti-APOA1 antibody [EPR25446-9] (BSA and Azide free) (AB300086)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise