AB231660

Anti-APOA4/Apo-AIV antibody

Name: Anti-APOA4/Apo-AIV antibody (ab231660) | Abcam
Brand: Abcam Limited
SKU: AB231660
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal APOA4/Apo-AIV antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Apoa4 aa 250 to C-terminus.

View Alternative Names

Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, Apoa4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4/Apo-AIV antibody (AB231660)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4/Apo-AIV antibody (AB231660)

Formalin-fixed, paraffin-embedded mouse intestine tissue stained for APOA4/Apo-AIV using ab231660 at 10 μg/ml in immunohistochemical analysis.

Supplier Data

Western blot - Anti-APOA4/Apo-AIV antibody (AB231660)

All lanes:

Western blot - Anti-APOA4/Apo-AIV antibody (ab231660) at 2 µg/mL

All lanes:

Mouse lung lysate

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-APOA4/Apo-AIV antibody (AB231660)

All lanes:

Western blot - Anti-APOA4/Apo-AIV antibody (ab231660) at 2 µg/mL

All lanes:

Recombinant mouse APOA4/Apo-AIV protein

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Apoa4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P06728

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231660 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOA4 also known as Apo-AIV or apolipoprotein A4 is a protein mainly expressed in the small intestine and liver. It has a molecular mass of approximately 46 kDa. This protein plays a significant role in lipid metabolism by facilitating the transport of lipids in the bloodstream. APOA4 exists in the plasma and associates predominately with high-density lipoproteins (HDL) and to a lesser extent with chylomicrons.

Biological function summary

Apolipoprotein A4 acts as an important player in the regulation of lipid homeostasis and satiety. This protein is not part of a complex but enhances the efficiency of lipid absorption and transport. APOA4 reacts with enzymes like lecithin-cholesterol acyltransferase which is essential in the maturation of HDL particles. This interaction supports cholesterol efflux and assists in maintaining cholesterol balance within the body.

Pathways

APOA4 is heavily involved in the reverse cholesterol transport pathway which is important for removing excess cholesterol from cells and tissues taking it back to the liver for excretion. Within this pathway APOA4 interacts with key proteins like apolipoprotein A1 and lecithin-cholesterol acyltransferase to aid in this lipid transport mechanism. It also participates in a related lipid homeostasis pathway enhancing lipid absorption efficiency.

Research links APOA4 to atherosclerosis and obesity. Altered expression or function of apolipoprotein A4 often correlates with increased cardiovascular disease risk due to its role in cholesterol transport. It is associated with apolipoprotein A1 as both of these proteins significantly impact lipid profiles related to cardiovascular health. The ability of APOA4 to influence satiety also connects it to obesity suggesting a role in energy balance regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

See full target information Apoa4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 3:1202-1216 PubMed34552271

2021

Diet-induced alteration of intestinal stem cell function underlies obesity and prediabetes in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Aliluev,Sophie Tritschler,Michael Sterr,Lena Oppenländer,Julia Hinterdobler,Tobias Greisle,Martin Irmler,Johannes Beckers,Na Sun,Axel Walch,Kerstin Stemmer,Alida Kindt,Jan Krumsiek,Matthias H Tschöp,Malte D Luecken,Fabian J Theis,Heiko Lickert,Anika Böttcher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APOA4/Apo-AIV antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4/Apo-AIV antibody (AB231660)

Western blot - Anti-APOA4/Apo-AIV antibody (AB231660)

Western blot - Anti-APOA4/Apo-AIV antibody (AB231660)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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