AB211867

Anti-APOA4/Apo-AIV antibody [CL0468]

Name: Anti-APOA4/Apo-AIV antibody [CL0468] (ab211867) | Abcam
Brand: Abcam Limited
SKU: AB211867
Price: 490 USD
Availability: InStock
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Anti-APOA4/Apo-AIV antibody [CL0468] (ab211867) is a mouse monoclonal antibody detecting APOA4/Apo-AIV in Western Blot, IHC-P. Suitable for Human.

View Alternative Names

Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4/Apo-AIV antibody [CL0468] (AB211867)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4/Apo-AIV antibody [CL0468] (AB211867)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4/Apo-AIV antibody [CL0468] (AB211867)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4/Apo-AIV antibody [CL0468] (AB211867)

Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4/Apo-AIV antibody [CL0468] (AB211867)

Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOA4/Apo-AIV antibody [CL0468] (AB211867)

Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue labeling APOA4/Apo-AIV with ab211867 at 1/200 dilution.

Supplier Data

Western blot - Anti-APOA4/Apo-AIV antibody [CL0468] (AB211867)

All lanes:

Western blot - Anti-APOA4/Apo-AIV antibody [CL0468] (ab211867) at 1/500 dilution

All lanes:

Human plasma

Predicted band size: 41 kDa,42 kDa,45 kDa,60 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0468

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human APOA4 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P06727

Epitope

Binds to an epitope located within the peptide sequence GHLSFLEKDL as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-APOA4/Apo-AIV antibody [CL0468] (ab211867) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of APOA4/Apo-AIV?
Anti-APOA4/Apo-AIV [CL0468] (ab211867) specifically detects a band for APOA4/Apo-AIV (UniProt: P06727) at a molecular weight of 45kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOA4 also known as Apo-AIV or apolipoprotein A4 is a protein mainly expressed in the small intestine and liver. It has a molecular mass of approximately 46 kDa. This protein plays a significant role in lipid metabolism by facilitating the transport of lipids in the bloodstream. APOA4 exists in the plasma and associates predominately with high-density lipoproteins (HDL) and to a lesser extent with chylomicrons.

Biological function summary

Apolipoprotein A4 acts as an important player in the regulation of lipid homeostasis and satiety. This protein is not part of a complex but enhances the efficiency of lipid absorption and transport. APOA4 reacts with enzymes like lecithin-cholesterol acyltransferase which is essential in the maturation of HDL particles. This interaction supports cholesterol efflux and assists in maintaining cholesterol balance within the body.

Pathways

APOA4 is heavily involved in the reverse cholesterol transport pathway which is important for removing excess cholesterol from cells and tissues taking it back to the liver for excretion. Within this pathway APOA4 interacts with key proteins like apolipoprotein A1 and lecithin-cholesterol acyltransferase to aid in this lipid transport mechanism. It also participates in a related lipid homeostasis pathway enhancing lipid absorption efficiency.

Research links APOA4 to atherosclerosis and obesity. Altered expression or function of apolipoprotein A4 often correlates with increased cardiovascular disease risk due to its role in cholesterol transport. It is associated with apolipoprotein A1 as both of these proteins significantly impact lipid profiles related to cardiovascular health. The ability of APOA4 to influence satiety also connects it to obesity suggesting a role in energy balance regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

See full target information APOA4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 4:589-607 PubMed35618940

2022

PARK7/DJ-1 promotes pyruvate dehydrogenase activity and maintains T homeostasis during ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Egle Danileviciute,Ni Zeng,Christophe M Capelle,Nicole Paczia,Mark A Gillespie,Henry Kurniawan,Mohaned Benzarti,Myriam P Merz,Djalil Coowar,Sabrina Fritah,Daniela Maria Vogt Weisenhorn,Gemma Gomez Giro,Melanie Grusdat,Alexandre Baron,Coralie Guerin,Davide G Franchina,Cathy Léonard,Olivia Domingues,Sylvie Delhalle,Wolfgang Wurst,Jonathan D Turner,Jens Christian Schwamborn,Johannes Meiser,Rejko Krüger,Jeff Ranish,Dirk Brenner,Carole L Linster,Rudi Balling,Markus Ollert,Feng Q Hefeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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