Rabbit Recombinant Monoclonal APOA4/Apo-AIV antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-APOA4/Apo-AIV antibody [EPR19465-232] - BSA and Azide free (Capture) (AB242651), expandable thumbnail](https://content.abcam.com/products/images/apoa4-apo-aiv-antibody-epr19465-232-bsa-and-azide-free-capture-ab242651--sandwich-elisa-img52505.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-APOA4/Apo-AIV antibody [EPR19465-232] - BSA and Azide free (Capture) (AB242651)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Alternative names
Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4
Recommended products
Rabbit Recombinant Monoclonal APOA4/Apo-AIV antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19465-232
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab242651 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Apolipoprotein A-IV. The recommended pair for sandwich ELISA is:
Capture: ab242651, Human Apolipoprotein A-IV Capture Antibody (unconjugated)
Detector: Anti-APOA4/Apo-AIV antibody [EPR19465-41] - BSA and Azide free (Detector) ab242896, Human Apolipoprotein A-IV Detector Antibody (unconjugated)
The reference range value is 250 - 16000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
APOA4 also known as Apo-AIV or apolipoprotein A4 is a protein mainly expressed in the small intestine and liver. It has a molecular mass of approximately 46 kDa. This protein plays a significant role in lipid metabolism by facilitating the transport of lipids in the bloodstream. APOA4 exists in the plasma and associates predominately with high-density lipoproteins (HDL) and to a lesser extent with chylomicrons.
- Biological function summary
Apolipoprotein A4 acts as an important player in the regulation of lipid homeostasis and satiety. This protein is not part of a complex but enhances the efficiency of lipid absorption and transport. APOA4 reacts with enzymes like lecithin-cholesterol acyltransferase which is essential in the maturation of HDL particles. This interaction supports cholesterol efflux and assists in maintaining cholesterol balance within the body.
- Pathways
APOA4 is heavily involved in the reverse cholesterol transport pathway which is important for removing excess cholesterol from cells and tissues taking it back to the liver for excretion. Within this pathway APOA4 interacts with key proteins like apolipoprotein A1 and lecithin-cholesterol acyltransferase to aid in this lipid transport mechanism. It also participates in a related lipid homeostasis pathway enhancing lipid absorption efficiency.
- Associated diseases and disorders
Research links APOA4 to atherosclerosis and obesity. Altered expression or function of apolipoprotein A4 often correlates with increased cardiovascular disease risk due to its role in cholesterol transport. It is associated with apolipoprotein A1 as both of these proteins significantly impact lipid profiles related to cardiovascular health. The ability of APOA4 to influence satiety also connects it to obesity suggesting a role in energy balance regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-APOA4/Apo-AIV antibody [EPR19465-232] - BSA and Azide free (Capture) (ab242651), expandable thumbnail](https://content.abcam.com/products/images/apoa4-apo-aiv-antibody-epr19465-232-bsa-and-azide-free-capture-ab242651--sandwich-elisa-img52505.jpg "Sandwich ELISA - Anti-APOA4/Apo-AIV antibody [EPR19465-232] - BSA and Azide free (Capture) (ab242651)")
Sandwich ELISA - Anti-APOA4/Apo-AIV antibody [EPR19465-232] - BSA and Azide free (Capture) (ab242651)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human Apolipoprotein A-IV ELISA Kit ab214567).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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