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AB247606

Anti-APOA4/Apo-AIV antibody [EPR2895] - BSA and Azide free

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Rabbit Recombinant Monoclonal APOA4/Apo-AIV antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4

2 Images
Immunoprecipitation - Anti-APOA4/Apo-AIV antibody [EPR2895] - BSA and Azide free (AB247606)
  • IP

Unknown

Immunoprecipitation - Anti-APOA4/Apo-AIV antibody [EPR2895] - BSA and Azide free (AB247606)

This data was developed using ab108200, the same antibody clone in a different buffer formulation.

Western blot analysis of APOA4/Apo-AIV in immunoprecipitation pellets from Human plasma (Lane 1) or 1XPBS (negative control) (Lane 2) using ab108200 at 1/10 dilution for IP and at 1/1000 dilution for WB detection.

All lanes:

Immunoprecipitation - Anti-APOA4/Apo-AIV antibody [EPR2895] (<a href='/en-us/products/primary-antibodies/apoa4-apo-aiv-antibody-epr2895-ab108200'>ab108200</a>)

Predicted band size: 45 kDa

false

Western blot - Anti-APOA4/Apo-AIV antibody [EPR2895] - BSA and Azide free (AB247606)
  • WB

Unknown

Western blot - Anti-APOA4/Apo-AIV antibody [EPR2895] - BSA and Azide free (AB247606)

This data was developed using ab108200, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-APOA4/Apo-AIV antibody [EPR2895] (<a href='/en-us/products/primary-antibodies/apoa4-apo-aiv-antibody-epr2895-ab108200'>ab108200</a>) at 1/1000 dilution

All lanes:

Human plasma at 10 µg

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2895

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab247606 is the carrier-free version of ab108200.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOA4 also known as Apo-AIV or apolipoprotein A4 is a protein mainly expressed in the small intestine and liver. It has a molecular mass of approximately 46 kDa. This protein plays a significant role in lipid metabolism by facilitating the transport of lipids in the bloodstream. APOA4 exists in the plasma and associates predominately with high-density lipoproteins (HDL) and to a lesser extent with chylomicrons.
Biological function summary

Apolipoprotein A4 acts as an important player in the regulation of lipid homeostasis and satiety. This protein is not part of a complex but enhances the efficiency of lipid absorption and transport. APOA4 reacts with enzymes like lecithin-cholesterol acyltransferase which is essential in the maturation of HDL particles. This interaction supports cholesterol efflux and assists in maintaining cholesterol balance within the body.

Pathways

APOA4 is heavily involved in the reverse cholesterol transport pathway which is important for removing excess cholesterol from cells and tissues taking it back to the liver for excretion. Within this pathway APOA4 interacts with key proteins like apolipoprotein A1 and lecithin-cholesterol acyltransferase to aid in this lipid transport mechanism. It also participates in a related lipid homeostasis pathway enhancing lipid absorption efficiency.

Research links APOA4 to atherosclerosis and obesity. Altered expression or function of apolipoprotein A4 often correlates with increased cardiovascular disease risk due to its role in cholesterol transport. It is associated with apolipoprotein A1 as both of these proteins significantly impact lipid profiles related to cardiovascular health. The ability of APOA4 to influence satiety also connects it to obesity suggesting a role in energy balance regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
See full target information APOA4
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Product promise

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