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Goat Polyclonal APOBEC1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human APOBEC1 aa 1-50.

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Images

Western blot - Anti-APOBEC1 antibody (AB80493), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human APOBEC1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P41238

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Predicted
Mouse
Tested
Orangutan
Predicted

Tested
Tested

Species

Mouse

Dilution info

1-3 µg/mL

Notes

1 hour primary incubation is recommended for this product.

Predicted
Predicted

Species

Human, Orangutan

Dilution info

-

Notes

-

Target data

Function

Cytidine deaminase catalyzing the cytidine to uridine postranscriptional editing of a variety of mRNAs (PubMed:30844405). Form complexes with cofactors that confer differential editing activity and selectivity. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the apolipoprotein B mRNA (PubMed:24916387). Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA (PubMed:11727199). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (By similarity).

Alternative names

Recommended products

Goat Polyclonal APOBEC1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human APOBEC1 aa 1-50.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human APOBEC1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P41238
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab80493 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

APOBEC1 also known as Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 1 is an enzyme responsible for the deamination of cytidine to uridine in mRNA. This editing capability focuses mainly on apolipoprotein B (apoB) mRNA affecting lipid metabolism significantly. The APOBEC1 protein weighs approximately 27 kDa. It expresses mainly in the small intestine and liver where lipid processing is an essential biological function.

Biological function summary

APOBEC1 plays an important role in lipid homeostasis by editing apoB mRNA transcripts leading to the production of a shorter form of the apoB protein. This protein acts as a critical component in lipid transport and metabolism. APOBEC1 functions within a multisubunit complex that includes other proteins like A1CF (APOBEC1 Complementation Factor). This protein complex ensures efficient mRNA editing necessary for optimal lipid regulation.

Pathways

The APOBEC1 enzyme is part of key metabolic routes notably lipid metabolism and lipid-transport routes. It contributes to the post-transcriptional modification of apoB mRNA influencing pathways like lipoprotein assembly. Through these pathways related proteins such as microsomal triglyceride transfer protein (MTP) work alongside APOBEC1 to facilitate lipid processing. MTP assists in lipoprotein biogenesis highlighting a collaborative function in lipid management.

Associated diseases and disorders

APOBEC1 has associations with conditions such as atherosclerosis and hepatic steatosis. Aberrant expression or function of APOBEC1 can lead to improper lipid metabolism directly linking to the development of atherosclerosis due to the accumulation of cholesterol-rich lipoproteins. Furthermore hepatic steatosis or fatty liver disease may arise when lipid homeostasis deteriorates. In these conditions APOBEC1 along with proteins like apoB becomes central to understanding disease mechanisms and potential therapeutic interventions.

Product promise

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1 product image

  • Western blot - Anti-APOBEC1 antibody (ab80493), expandable thumbnail

    Western blot - Anti-APOBEC1 antibody (ab80493)

    Primary incubation was 1 hour.

    All lanes: Western blot - Anti-APOBEC1 antibody (ab80493) at 1 µg/mL

    All lanes: mouse spleen lysate (in RIPA buffer) at 35 µg

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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