Goat Polyclonal APOBEC1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human APOBEC1 aa 1-50.
IgG
Goat
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Liquid
Polyclonal
WB | |
---|---|
Human | Predicted |
Mouse | Tested |
Orangutan | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1-3 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species | Dilution info | Notes |
---|---|---|
Species Human, Orangutan | Dilution info - | Notes - |
Cytidine deaminase catalyzing the cytidine to uridine postranscriptional editing of a variety of mRNAs (PubMed:30844405). Form complexes with cofactors that confer differential editing activity and selectivity. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the apolipoprotein B mRNA (PubMed:24916387). Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA (PubMed:11727199). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (By similarity).
C->U-editing enzyme APOBEC-1, Apolipoprotein B mRNA-editing enzyme catalytic subunit 1, HEPR, mRNA(cytosine(6666)) deaminase 1, APO1, APOBEC-1, Apolipoprotein B mRNA-editing enzyme 1, APOBEC1
Goat Polyclonal APOBEC1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human APOBEC1 aa 1-50.
IgG
Goat
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Liquid
Polyclonal
Affinity purification Immunogen
ab80493 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This supplementary information is collated from multiple sources and compiled automatically.
APOBEC1 also known as Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 1 is an enzyme responsible for the deamination of cytidine to uridine in mRNA. This editing capability focuses mainly on apolipoprotein B (apoB) mRNA affecting lipid metabolism significantly. The APOBEC1 protein weighs approximately 27 kDa. It expresses mainly in the small intestine and liver where lipid processing is an essential biological function.
APOBEC1 plays an important role in lipid homeostasis by editing apoB mRNA transcripts leading to the production of a shorter form of the apoB protein. This protein acts as a critical component in lipid transport and metabolism. APOBEC1 functions within a multisubunit complex that includes other proteins like A1CF (APOBEC1 Complementation Factor). This protein complex ensures efficient mRNA editing necessary for optimal lipid regulation.
The APOBEC1 enzyme is part of key metabolic routes notably lipid metabolism and lipid-transport routes. It contributes to the post-transcriptional modification of apoB mRNA influencing pathways like lipoprotein assembly. Through these pathways related proteins such as microsomal triglyceride transfer protein (MTP) work alongside APOBEC1 to facilitate lipid processing. MTP assists in lipoprotein biogenesis highlighting a collaborative function in lipid management.
APOBEC1 has associations with conditions such as atherosclerosis and hepatic steatosis. Aberrant expression or function of APOBEC1 can lead to improper lipid metabolism directly linking to the development of atherosclerosis due to the accumulation of cholesterol-rich lipoproteins. Furthermore hepatic steatosis or fatty liver disease may arise when lipid homeostasis deteriorates. In these conditions APOBEC1 along with proteins like apoB becomes central to understanding disease mechanisms and potential therapeutic interventions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Primary incubation was 1 hour.
All lanes: Western blot - Anti-APOBEC1 antibody (ab80493) at 1 µg/mL
All lanes: mouse spleen lysate (in RIPA buffer) at 35 µg
Predicted band size: 28 kDa
Observed band size: 28 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com