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AB80493

Anti-APOBEC1 antibody

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(1 Publication)

Goat Polyclonal APOBEC1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human APOBEC1 aa 1-50.

View Alternative Names

C->U-editing enzyme APOBEC-1, Apolipoprotein B mRNA-editing enzyme catalytic subunit 1, HEPR, mRNA(cytosine(6666)) deaminase 1, APO1, APOBEC-1, Apolipoprotein B mRNA-editing enzyme 1, APOBEC1

1 Images
Western blot - Anti-APOBEC1 antibody (AB80493)
  • WB

Unknown

Western blot - Anti-APOBEC1 antibody (AB80493)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-APOBEC1 antibody (ab80493) at 1 µg/mL

All lanes:

mouse spleen lysate (in RIPA buffer) at 35 µg

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human APOBEC1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P41238

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab80493 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC1 also known as Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 1 is an enzyme responsible for the deamination of cytidine to uridine in mRNA. This editing capability focuses mainly on apolipoprotein B (apoB) mRNA affecting lipid metabolism significantly. The APOBEC1 protein weighs approximately 27 kDa. It expresses mainly in the small intestine and liver where lipid processing is an essential biological function.
Biological function summary

APOBEC1 plays an important role in lipid homeostasis by editing apoB mRNA transcripts leading to the production of a shorter form of the apoB protein. This protein acts as a critical component in lipid transport and metabolism. APOBEC1 functions within a multisubunit complex that includes other proteins like A1CF (APOBEC1 Complementation Factor). This protein complex ensures efficient mRNA editing necessary for optimal lipid regulation.

Pathways

The APOBEC1 enzyme is part of key metabolic routes notably lipid metabolism and lipid-transport routes. It contributes to the post-transcriptional modification of apoB mRNA influencing pathways like lipoprotein assembly. Through these pathways related proteins such as microsomal triglyceride transfer protein (MTP) work alongside APOBEC1 to facilitate lipid processing. MTP assists in lipoprotein biogenesis highlighting a collaborative function in lipid management.

APOBEC1 has associations with conditions such as atherosclerosis and hepatic steatosis. Aberrant expression or function of APOBEC1 can lead to improper lipid metabolism directly linking to the development of atherosclerosis due to the accumulation of cholesterol-rich lipoproteins. Furthermore hepatic steatosis or fatty liver disease may arise when lipid homeostasis deteriorates. In these conditions APOBEC1 along with proteins like apoB becomes central to understanding disease mechanisms and potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytidine deaminase catalyzing the cytidine to uridine postranscriptional editing of a variety of mRNAs (PubMed : 30844405). Form complexes with cofactors that confer differential editing activity and selectivity. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the apolipoprotein B mRNA (PubMed : 24916387). Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA (PubMed : 11727199). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (By similarity).
See full target information APOBEC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 15:903-10 PubMed24916387

2014

C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Fossat,Karin Tourle,Tania Radziewic,Kristen Barratt,Doreen Liebhold,Joshua B Studdert,Melinda Power,Vanessa Jones,David A F Loebel,Patrick P L Tam
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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