AB170859

Anti-APOBEC2 antibody [EPR11286(B)]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal APOBEC2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

C->U-editing enzyme APOBEC-2, mRNA(cytosine(6666)) deaminase 2, APOBEC2

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

ab170859 showing -ve staining in Human normal liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

ab170859 showing -ve staining in Human thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

Immunohistochemical analysis of Formalin-fixed paraffin-embedded Human heart tissue labeling APOBEC2 with ab170859 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

Immunohistochemical analysis of Formalin-fixed paraffin-embedded Human skeletal muscle tissue labeling APOBEC2 with ab170859 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

ab170859 showing -ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

ab170859 showing -ve staining in Human cervical carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

WB

Supplier Data

Western blot - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859)

All lanes:

Western blot - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859) at 1/10000 dilution

Lane 1:

Human fetal muscle lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11286(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC2 short for Apolipoprotein B mRNA Editing Catalytic Polypeptide-like 2 functionally operates as a C to U RNA-editing enzyme. It belongs to the APOBEC family and has a molecular mass of approximately 26 kDa. Expression of APOBEC2 is mainly in heart and skeletal muscle tissues. Its sequence shares homology with other members of the deaminase family suggesting common ancestral functions.

Biological function summary

APOBEC2 influences muscle differentiation by contributing to RNA editing processes. This protein does not act as part of any known large multiprotein complex but interacts with other cellular components to perform its tasks. By changing specific RNA sequences APOBEC2 can affect the proteins generated in muscle cells influencing muscle fiber formation and maintenance.

Pathways

APOBEC2 plays a role in muscle-related pathways such as myogenesis where it is known to regulate muscle-specific gene expression. The involvement of APOBEC2 in the RNA processing pathway links it with activities concerning the RNA life cycle. It interacts with other members of the APOBEC family such as APOBEC1 which have roles in maintaining genomic integrity and modifying RNA to regulate gene expression.

APOBEC2 shows connection primarily to muscle-related pathologies. Deficiencies or dysfunctions in APOBEC2 can relate to myopathies disorders where muscle fibers do not function properly leading to muscle weakness. The protein is also investigated in the context of muscular dystrophy where its role in muscle maintenance suggests potential implications. In these contexts alterations in APOBEC2 can influence interactions with other muscle-related proteins impacting muscle health and disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.

See full target information APOBEC2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational neuroscience 15:20220357 PubMed39434773

2024

Cystatin C alleviates unconjugated bilirubin-induced neurotoxicity by promoting bilirubin clearance from neurocytes via exosomes, dependent on hepatocyte UGT1A1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yating Du,Zhenkun Li

BMC cancer 24:15 PubMed38166744

2024

Expression and prognostic value of APOBEC2 in gastric adenocarcinoma and its association with tumor-infiltrating immune cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lipan Wei,Xiuqian Wu,Lan Wang,Ling Chen,Xuejun Wu,Tiantian Song,Yuanyuan Wang,Wenjun Chang,Aizhen Guo,Yongdong Niu,Haihua Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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