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Rabbit Recombinant Monoclonal APOBEC2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (AB170859), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Mouse
Predicted
Not recommended
Expected
Not recommended
Rat
Predicted
Not recommended
Expected
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.

Alternative names

C->U-editing enzyme APOBEC-2, mRNA(cytosine(6666)) deaminase 2, APOBEC2

Recommended products

Rabbit Recombinant Monoclonal APOBEC2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR11286(B)
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

APOBEC2 short for Apolipoprotein B mRNA Editing Catalytic Polypeptide-like 2 functionally operates as a C to U RNA-editing enzyme. It belongs to the APOBEC family and has a molecular mass of approximately 26 kDa. Expression of APOBEC2 is mainly in heart and skeletal muscle tissues. Its sequence shares homology with other members of the deaminase family suggesting common ancestral functions.

Biological function summary

APOBEC2 influences muscle differentiation by contributing to RNA editing processes. This protein does not act as part of any known large multiprotein complex but interacts with other cellular components to perform its tasks. By changing specific RNA sequences APOBEC2 can affect the proteins generated in muscle cells influencing muscle fiber formation and maintenance.

Pathways

APOBEC2 plays a role in muscle-related pathways such as myogenesis where it is known to regulate muscle-specific gene expression. The involvement of APOBEC2 in the RNA processing pathway links it with activities concerning the RNA life cycle. It interacts with other members of the APOBEC family such as APOBEC1 which have roles in maintaining genomic integrity and modifying RNA to regulate gene expression.

Associated diseases and disorders

APOBEC2 shows connection primarily to muscle-related pathologies. Deficiencies or dysfunctions in APOBEC2 can relate to myopathies disorders where muscle fibers do not function properly leading to muscle weakness. The protein is also investigated in the context of muscular dystrophy where its role in muscle maintenance suggests potential implications. In these contexts alterations in APOBEC2 can influence interactions with other muscle-related proteins impacting muscle health and disease outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

    Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human skeletal muscle tissue labeling APOBEC2 with ab170859 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

    Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human heart tissue labeling APOBEC2 with ab170859 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

    ab170859 showing -ve staining in Human thyroid gland carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

    ab170859 showing -ve staining in Human normal liver tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

    ab170859 showing -ve staining in Human normal kidney tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

    ab170859 showing -ve staining in Human cervical carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859), expandable thumbnail

    Western blot - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859)

    All lanes: Western blot - Anti-APOBEC2 antibody [EPR11286(B)] (ab170859) at 1/10000 dilution

    Lane 1: Human fetal muscle lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Predicted band size: 26 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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