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AB249542

Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal APOBEC2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

C->U-editing enzyme APOBEC-2, mRNA(cytosine(6666)) deaminase 2, APOBEC2

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)

This data was developed using ab170859, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human skeletal muscle tissue labeling APOBEC2 with ab170859 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)

This data was developed using ab170859, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human heart tissue labeling APOBEC2 with ab170859 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)

This data was developed using ab170859, the same antibody clone in a different buffer formulation.

ab170859 showing -ve staining in Human thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)

This data was developed using ab170859, the same antibody clone in a different buffer formulation.

ab170859 showing -ve staining in Human normal liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)

This data was developed using ab170859, the same antibody clone in a different buffer formulation.

ab170859 showing -ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)

This data was developed using ab170859, the same antibody clone in a different buffer formulation.

ab170859 showing -ve staining in Human cervical carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)
  • WB

Supplier Data

Western blot - Anti-APOBEC2 antibody [EPR11286(B)] - BSA and Azide free (AB249542)

This data was developed using ab170859, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-APOBEC2 antibody [EPR11286(B)] (<a href='/en-us/products/primary-antibodies/apobec2-antibody-epr11286b-ab170859'>ab170859</a>) at 1/10000 dilution

Lane 1:

Human fetal muscle lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11286(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249542 is the carrier-free version of ab170859.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC2 short for Apolipoprotein B mRNA Editing Catalytic Polypeptide-like 2 functionally operates as a C to U RNA-editing enzyme. It belongs to the APOBEC family and has a molecular mass of approximately 26 kDa. Expression of APOBEC2 is mainly in heart and skeletal muscle tissues. Its sequence shares homology with other members of the deaminase family suggesting common ancestral functions.
Biological function summary

APOBEC2 influences muscle differentiation by contributing to RNA editing processes. This protein does not act as part of any known large multiprotein complex but interacts with other cellular components to perform its tasks. By changing specific RNA sequences APOBEC2 can affect the proteins generated in muscle cells influencing muscle fiber formation and maintenance.

Pathways

APOBEC2 plays a role in muscle-related pathways such as myogenesis where it is known to regulate muscle-specific gene expression. The involvement of APOBEC2 in the RNA processing pathway links it with activities concerning the RNA life cycle. It interacts with other members of the APOBEC family such as APOBEC1 which have roles in maintaining genomic integrity and modifying RNA to regulate gene expression.

APOBEC2 shows connection primarily to muscle-related pathologies. Deficiencies or dysfunctions in APOBEC2 can relate to myopathies disorders where muscle fibers do not function properly leading to muscle weakness. The protein is also investigated in the context of muscular dystrophy where its role in muscle maintenance suggests potential implications. In these contexts alterations in APOBEC2 can influence interactions with other muscle-related proteins impacting muscle health and disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
See full target information APOBEC2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com