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AB222330

Anti-APOBEC3B antibody

5

(3 Reviews)

|

(3 Publications)

Rabbit Polyclonal APOBEC3B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human APOBEC3B aa 250-300.

View Alternative Names

DNA dC->dU-editing enzyme APOBEC-3B, A3B, Phorbolin-1-related protein, Phorbolin-2/3, APOBEC3B

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-APOBEC3B antibody (AB222330)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-APOBEC3B antibody (AB222330)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for APOBEC3B (green) using ab222330 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human APOBEC3B aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UH17

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC3B also known as Apolipoprotein B mRNA editing enzyme catalytic subunit 3B is a protein that plays a role in DNA cytosine deamination. It selectively deaminates cytosine bases in DNA to uracil resulting in mutations. The protein has a molecular mass of approximately 46 kDa. APOBEC3B expression occurs primarily in the liver and testes but it can also be detected in other tissues.
Biological function summary

APOBEC3B functions as part of the innate immune defense by restricting viral replication through inducing hypermutation in viral genomes. It does not form a stable complex but can act in conjunction with other APOBEC3 family members. APOBEC3B activity is critical in the context of host-virus interactions especially in the defense against retroviruses and other viruses.

Pathways

Scientists classify APOBEC3B within the viral restriction and DNA damage response pathways. In these pathways the protein works alongside other DNA repair proteins such as AID (Activation-Induced cytidine Deaminase). APOBEC3B participates in editing events that provide insights into virus-host co-evolution and resilience against viral infections.

Researchers have linked APOBEC3B to cancer development due to its ability to induce genetic mutations. Its mutagenic activity connects it with a variety of cancers including breast cancer and head and neck cancer. Through these diseases the protein shows functional connections to other DNA mutators and repair proteins that it affects or interacts with during tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons.
See full target information APOBEC3B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40647481

2025

miR-195-5p Suppresses KRT80 Expression Inducing Cell Cycle Arrest in Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuele Piccinno,Viviana Scalavino,Nicoletta Labarile,Giusy Bianco,Raffaele Armentano,Gianluigi Giannelli,Grazia Serino

Oncotarget 11:3354-3370 PubMed32934779

2020

GATA3 and APOBEC3B are prognostic markers in adrenocortical carcinoma and APOBEC3B is directly transcriptionally regulated by GATA3.

Applications

Unspecified application

Species

Unspecified reactive species

Sudheer Kumar Gara,Monica Varun Tyagi,Dhaval Thakkur Patel,Kelli Gaskins,Justin Lack,Yi Liu,Electron Kebebew

Molecular carcinogenesis 58:643-653 PubMed30575099

2018

APOBEC3B promotes hepatocarcinogenesis and metastasis through novel deaminase-independent activity.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Ma,Daniel W-H Ho,Karen M-F Sze,Yu-Man Tsui,Lo-Kong Chan,Joyce M-F Lee,Irene O-L Ng
View all publications
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