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Rabbit Recombinant Monoclonal APOBEC3B antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples. Cited in 14 publications.


Images

Western blot - Anti-APOBEC3B antibody [EPR18138] (AB184990), expandable thumbnail
  • Western blot - Anti-APOBEC3B antibody [EPR18138] (AB184990), expandable thumbnail
  • Western blot - Anti-APOBEC3B antibody [EPR18138] (AB184990), expandable thumbnail
  • Western blot - Anti-APOBEC3B antibody [EPR18138] (AB184990), expandable thumbnail
  • Western blot - Anti-APOBEC3B antibody [EPR18138] (AB184990), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Transfected cell lysate - Human
Tested

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/500 - 1/10000
Notes

-

Species
Human
Dilution info
1/500 - 1/10000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons.

Alternative names

DNA dC->dU-editing enzyme APOBEC-3B, A3B, Phorbolin-1-related protein, Phorbolin-2/3, APOBEC3B

Recommended products

Rabbit Recombinant Monoclonal APOBEC3B antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples. Cited in 14 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18138
Purification technique
Affinity purification Protein A
Specificity

An additional band is detected in Western Blot by ab184990 at 50/55kD in most samples. We do not know the identity of this band.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

APOBEC3B also known as Apolipoprotein B mRNA editing enzyme catalytic subunit 3B is a protein that plays a role in DNA cytosine deamination. It selectively deaminates cytosine bases in DNA to uracil resulting in mutations. The protein has a molecular mass of approximately 46 kDa. APOBEC3B expression occurs primarily in the liver and testes but it can also be detected in other tissues.

Biological function summary

APOBEC3B functions as part of the innate immune defense by restricting viral replication through inducing hypermutation in viral genomes. It does not form a stable complex but can act in conjunction with other APOBEC3 family members. APOBEC3B activity is critical in the context of host-virus interactions especially in the defense against retroviruses and other viruses.

Pathways

Scientists classify APOBEC3B within the viral restriction and DNA damage response pathways. In these pathways the protein works alongside other DNA repair proteins such as AID (Activation-Induced cytidine Deaminase). APOBEC3B participates in editing events that provide insights into virus-host co-evolution and resilience against viral infections.

Associated diseases and disorders

Researchers have linked APOBEC3B to cancer development due to its ability to induce genetic mutations. Its mutagenic activity connects it with a variety of cancers including breast cancer and head and neck cancer. Through these diseases the protein shows functional connections to other DNA mutators and repair proteins that it affects or interacts with during tumorigenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990), expandable thumbnail

    Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution

    Lane 1: Human APOBEC3B fragment recombinant protein at 0.01 µg

    Lane 2: Human APOBEC3G fragment recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 46 kDa

    Observed band size: 46 kDa

  • Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990), expandable thumbnail

    Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 1% NFDM/TBST.

    Exposure time: Panel A: 20 s; Panel B: 1 min.

    Figure provided by Dr. Jenni Nikkila. Gene Function Lab at The Institute of Cancer Research, London. HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line provide by Dr Reuben Harris, University of Minnesota, Minneapolis - cited in Akre, M.K, G.J. Starrett, J.S. Quist, N.A. Temiz, M.A. Carpenter, A.N.J. Tutt, A. Grigoriadis & R.S. Harris (2016) Mutation processes in 293-based clones overexpressing the DNA cytosine deaminase APOBEC3B. PLoS One11(5):e0155391. doi: 10.1371/journal.pone.0155391.

    The 35 kDa band represents a known splice variant of APOBEC3B.

    All lanes: Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/10000 dilution

    Lane 1: HepaRG-APOBEC3B tetracycline-inducible cell line (+/-) at 20 µg

    Lane 2: HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-) at 20 µg

    Lane 3: HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line (+/-) at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (H+L) conjugated to HRP at 1/10000 dilution

    Predicted band size: 46 kDa

    Observed band size: 35 kDa, 70 kDa

  • Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990), expandable thumbnail

    Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

    APOBEC3B Western blot staining using rabbit Anti-APOBEC3B antibody

    Negative sample: SK-BR-3 (PMID: 27634334).

    Merged signal (red and green).

    Green - ab184990 observed at 38 kDa.

    Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.


    ab184990 Anti-APOBEC3B antibody [EPR18138] was shown to specifically react with APOBEC3B in wild-type HeLa cells. Loss of signal was observed when knockout cell line APOBEC3B knockout HeLa was used. Wild-type and APOBEC3B knockout samples were subjected to SDS-PAGE.

    ab184990 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively.

    Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: APOBEC3B knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Lanes 1 - 4: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution

    Observed band size: 38 kDa

  • Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990), expandable thumbnail

    Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

    APOBEC3B Western blot staining using rabbit Anti-APOBEC3B antibody

    Exposure time: Lane 1-3: 20 seconds; Lane 4-5: 180 seconds

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.

    ab184990 only detects very weak signal in HepG2 and HeLa cell lines.

    All lanes: Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution

    Lane 1: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 2 and 4: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 3 and 5: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 38 kDa

  • Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990), expandable thumbnail

    Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM /TBST
    We are unsure of the identity of the 55kda band.

    All lanes: Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/500 dilution

    All lanes: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa

    Observed band size: 38 kDa

    Exposure time: 180s

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