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Rabbit Recombinant Monoclonal APOBEC3B antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples. Cited in 14 publications.


Images

Western blot - Anti-APOBEC3B antibody [EPR18138] (AB184990), expandable thumbnail
  • Western blot - Anti-APOBEC3B antibody [EPR18138] (AB184990), expandable thumbnail
  • Western blot - Anti-APOBEC3B antibody [EPR18138] (AB184990), expandable thumbnail

Publications

  • Nature 607:799-8072022
    Mechanisms of APOBEC3 mutagenesis in human cancer cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Mia Petljak,Alexandra Dananberg,Kevan Chu,Erik N Bergstrom,Josefine Striepen,Patrick von Morgen,Yanyang Chen,Hina Shah,Julian E Sale,Ludmil B Alexandrov,Michael R Stratton,John Maciejowski
    PubMed 35859169
  • Frontiers in oncology 11:6581392021
    CircNRIP1 Encapsulated by Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Aggravates Osteosarcoma by Modulating the miR-532-3p/AKT3/PI3K/AKT Axis.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Zuowei Shi,Kaifu Wang,Yufei Xing,Xuefeng Yang
    PubMed 34660257

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Transfected cell lysate - Human
Tested

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

1/500 - 1/10000

Notes

-

Species

Human

Dilution info

1/500 - 1/10000

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons.

      Alternative names

      DNA dC->dU-editing enzyme APOBEC-3B, A3B, Phorbolin-1-related protein, Phorbolin-2/3, APOBEC3B

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      Rabbit Recombinant Monoclonal APOBEC3B antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples. Cited in 14 publications.

      Alternative names

      DNA dC->dU-editing enzyme APOBEC-3B, A3B, Phorbolin-1-related protein, Phorbolin-2/3, APOBEC3B

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR18138

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      APOBEC3B also known as Apolipoprotein B mRNA editing enzyme catalytic subunit 3B is a protein that plays a role in DNA cytosine deamination. It selectively deaminates cytosine bases in DNA to uracil resulting in mutations. The protein has a molecular mass of approximately 46 kDa. APOBEC3B expression occurs primarily in the liver and testes but it can also be detected in other tissues.

      Biological function summary

      APOBEC3B functions as part of the innate immune defense by restricting viral replication through inducing hypermutation in viral genomes. It does not form a stable complex but can act in conjunction with other APOBEC3 family members. APOBEC3B activity is critical in the context of host-virus interactions especially in the defense against retroviruses and other viruses.

      Pathways

      Scientists classify APOBEC3B within the viral restriction and DNA damage response pathways. In these pathways the protein works alongside other DNA repair proteins such as AID (Activation-Induced cytidine Deaminase). APOBEC3B participates in editing events that provide insights into virus-host co-evolution and resilience against viral infections.

      Associated diseases and disorders

      Researchers have linked APOBEC3B to cancer development due to its ability to induce genetic mutations. Its mutagenic activity connects it with a variety of cancers including breast cancer and head and neck cancer. Through these diseases the protein shows functional connections to other DNA mutators and repair proteins that it affects or interacts with during tumorigenesis.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      3 product images

      • Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990), expandable thumbnail

        Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        All lanes: Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution

        Lane 1: Human APOBEC3B fragment recombinant protein at 0.01 µg

        Lane 2: Human APOBEC3G fragment recombinant protein at 0.01 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

        Predicted band size: 46 kDa

        Observed band size: 46 kDa

      • Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990), expandable thumbnail

        Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

        Blocking buffer: 5% NFDM/TBST.

        Dilution buffer: 1% NFDM/TBST.

        Exposure time: Panel A: 20 s; Panel B: 1 min.

        Figure provided by Dr. Jenni Nikkila. Gene Function Lab at The Institute of Cancer Research, London. HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line provide by Dr Reuben Harris, University of Minnesota, Minneapolis - cited in Akre, M.K, G.J. Starrett, J.S. Quist, N.A. Temiz, M.A. Carpenter, A.N.J. Tutt, A. Grigoriadis & R.S. Harris (2016) Mutation processes in 293-based clones overexpressing the DNA cytosine deaminase APOBEC3B. PLoS One11(5):e0155391. doi: 10.1371/journal.pone.0155391.

        The 35 kDa band represents a known splice variant of APOBEC3B.

        All lanes: Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/10000 dilution

        Lane 1: HepaRG-APOBEC3B tetracycline-inducible cell line (+/-) at 20 µg

        Lane 2: HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-) at 20 µg

        Lane 3: HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line (+/-) at 20 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG (H+L) conjugated to HRP at 1/10000 dilution

        Predicted band size: 46 kDa

        Observed band size: 35 kDa, 70 kDa

      • Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990), expandable thumbnail

        Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

        Blocking buffer and concentration: 5% NFDM/TBST
        Diluting buffer and concentration: 5% NFDM /TBST
        We are unsure of the identity of the 55kda band.

        All lanes: Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/500 dilution

        All lanes: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 46 kDa

        Observed band size: 38 kDa

        Exposure time: 180s

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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