AB250804

Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal APOBEC3B antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Human samples. Cited in 1 publication.

View Alternative Names

DNA dC->dU-editing enzyme APOBEC-3B, A3B, Phorbolin-1-related protein, Phorbolin-2/3, APOBEC3B

5 Images

Lab

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

This data was developed using ab184990, the same antibody clone in a different buffer formulation.

Negative sample : SK-BR-3 (PMID : 27634334).

Merged signal (red and green).
Green - ab184990 observed at 38 kDa.
Red - loading control, ab8245 observed at 36 kDa.

ab184990 Anti-APOBEC3B antibody [EPR18138] was shown to specifically react with APOBEC3B in wild-type HeLa cells. Loss of signal was observed when APOBEC3B CRISPR-Edited HeLa cell line was used. Wild-type and APOBEC3B CRISPR-Edited HeLa samples were subjected to SDS-PAGE.

ab184990 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively.

Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-APOBEC3B antibody [EPR18138] (<a href='/en-us/products/primary-antibodies/apobec3b-antibody-epr18138-ab184990'>ab184990</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

APOBEC3B CRISPR-Edited HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Lanes 1 - 4:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

Observed band size: 38 kDa

false

Lab

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM /TBST We are unsure of the identity of the 55kda band. This data was developed using ab184990, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-APOBEC3B antibody [EPR18138] (<a href='/en-us/products/primary-antibodies/apobec3b-antibody-epr18138-ab184990'>ab184990</a>) at 1/500 dilution

All lanes:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 38 kDa

false

Exposure time: 180s

Collaborator

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

This data was developed using ab184990, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Panel A : 20 s; Panel B : 1 min.

Figure provided by Dr. Jenni Nikkila. Gene Function Lab at The Institute of Cancer Research, London. HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line provide by Dr Reuben Harris, University of Minnesota, Minneapolis - cited in Akre, M.K, G.J. Starrett, J.S. Quist, N.A. Temiz, M.A. Carpenter, A.N.J. Tutt, A. Grigoriadis & R.S. Harris (2016) Mutation processes in 293-based clones overexpressing the DNA cytosine deaminase APOBEC3B. PLoS One11(5) : e0155391. doi : 10.1371/journal.pone.0155391.

The 35 kDa band represents a known splice variant of APOBEC3B.

All lanes:

Western blot - Anti-APOBEC3B antibody [EPR18138] (<a href='/en-us/products/primary-antibodies/apobec3b-antibody-epr18138-ab184990'>ab184990</a>) at 1/10000 dilution

Lane 1:

HepaRG-APOBEC3B tetracycline-inducible cell line (+/-) at 20 µg

Lane 2:

HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-) at 20 µg

Lane 3:

HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line (+/-) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L) conjugated to HRP at 1/10000 dilution

Predicted band size: 46 kDa

Observed band size: 35 kDa,70 kDa

false

Lab

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

This data was developed using ab184990, the same antibody clone in a different buffer formulation.

Exposure time : Lane 1-3 : 20 seconds; Lane 4-5 : 180 seconds

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

ab184990 only detects very weak signal in HepG2 and HeLa cell lines.

All lanes:

Western blot - Anti-APOBEC3B antibody [EPR18138] (<a href='/en-us/products/primary-antibodies/apobec3b-antibody-epr18138-ab184990'>ab184990</a>) at 1/1000 dilution

Lane 1:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 2 and 4:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 3 and 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 38 kDa

false

Supplier Data

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

This data was developed using ab184990, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-APOBEC3B antibody [EPR18138] (<a href='/en-us/products/primary-antibodies/apobec3b-antibody-epr18138-ab184990'>ab184990</a>) at 1/1000 dilution

Lane 1:

Human APOBEC3B fragment recombinant protein at 0.01 µg

Lane 2:

Human APOBEC3G fragment recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Unconjugated

Anti-APOBEC3B antibody [EPR18138]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18138

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

An additional band is detected in Western Blot by ab184990 at 50/55kD in most samples. We do not know the identity of this band.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab250804 is the carrier-free version of ab184990.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC3B also known as Apolipoprotein B mRNA editing enzyme catalytic subunit 3B is a protein that plays a role in DNA cytosine deamination. It selectively deaminates cytosine bases in DNA to uracil resulting in mutations. The protein has a molecular mass of approximately 46 kDa. APOBEC3B expression occurs primarily in the liver and testes but it can also be detected in other tissues.

Biological function summary

APOBEC3B functions as part of the innate immune defense by restricting viral replication through inducing hypermutation in viral genomes. It does not form a stable complex but can act in conjunction with other APOBEC3 family members. APOBEC3B activity is critical in the context of host-virus interactions especially in the defense against retroviruses and other viruses.

Pathways

Scientists classify APOBEC3B within the viral restriction and DNA damage response pathways. In these pathways the protein works alongside other DNA repair proteins such as AID (Activation-Induced cytidine Deaminase). APOBEC3B participates in editing events that provide insights into virus-host co-evolution and resilience against viral infections.

Researchers have linked APOBEC3B to cancer development due to its ability to induce genetic mutations. Its mutagenic activity connects it with a variety of cancers including breast cancer and head and neck cancer. Through these diseases the protein shows functional connections to other DNA mutators and repair proteins that it affects or interacts with during tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons.

See full target information APOBEC3B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

Western blot - Anti-APOBEC3B antibody [EPR18138] - BSA and Azide free (AB250804)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise