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AB221874

Anti-APOBEC3C/PBI antibody

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(2 Publications)

Rabbit Polyclonal APOBEC3C/PBI antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human APOBEC3C aa 1-50.

View Alternative Names

APOBEC1L, PBI, APOBEC3C, DNA dC->dU-editing enzyme APOBEC-3C, A3C, APOBEC1-like, Phorbolin I

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-APOBEC3C/PBI antibody (AB221874)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-APOBEC3C/PBI antibody (AB221874)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for APOBEC3C/PBI (green) using ab221874 at 4 ug/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human APOBEC3C aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9NRW3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Gorilla": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC3C also known as 'PBI courier' is an enzyme that belongs to the cytidine deaminase family. This enzyme has a molecular weight of about 23 kDa. APOBEC3C functions by catalyzing the deamination of cytidine to uridine on single-stranded DNA. This process leads to mutations in viral DNA contributing to their inactivation. APOBEC3C is widely expressed in the human body with higher expression observed in immune-related tissues such as the spleen lymph nodes and peripheral blood leukocytes.
Biological function summary

APOBEC3C plays a role in the innate immune defense against viral infections. It belongs to a larger family of APOBEC3 enzymes which often work together as a complex to provide antiviral activity. The APOBEC3C enzyme contributes to the restriction of certain retroviruses and endogenous retroelements by introducing hypermutations into their genomes during replication. The presence of multiple APOBEC3 proteins within this complex suggests redundancy and diverse antiviral functions in human immunity.

Pathways

APOBEC3C is involved in the innate immune response pathway which recognizes and responds to pathogen infections including viral invasions. It interacts with other members of the APOBEC3 family such as APOBEC3G and APOBEC3B forming an elaborate network that defends the host genome from viral invasion. The enzymatic activity of APOBEC3C fits into the restriction pathway providing a protective mechanism against viral infections and maintaining genomic stability.

APOBEC3C's hypermutation activity is connected to the progression of HIV-1 where the virus may evolve escape mutants. Additionally the enzymatic action of APOBEC3C has been implicated in cancer especially in relation to the mutation profiles found in some tumor types. The interplay with other APOBEC3 proteins like APOBEC3B increases the complexity of their involvement in cancer mutagenesis and antiviral immunity highlighting the enzyme's dual role in protecting against pathogens and potentially promoting genomic instability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV), herpes simplex virus 1 (HHV-1) and Epstein-Barr virus (EBV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
See full target information APOBEC3C
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:24875 PubMed39438515

2024

Validation of a functional human AD model with four AD therapeutics utilizing patterned ipsc-derived cortical neurons integrated with microelectrode arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Julbert Caneus,Kaveena Autar,Nesar Akanda,Marcella Grillo,Christopher J Long,Max Jackson,Sarah Lindquist,Xiufang Guo,Dave Morgan,James J Hickman

Cell reports 34:108670 PubMed33503434

2021

Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolution.

Applications

Unspecified application

Species

Unspecified reactive species

Qingfei Jiang,Jane Isquith,Luisa Ladel,Adam Mark,Frida Holm,Cayla Mason,Yudou He,Phoebe Mondala,Isabelle Oliver,Jessica Pham,Wenxue Ma,Eduardo Reynoso,Shawn Ali,Isabella Jamieson Morris,Raymond Diep,Chanond Nasamran,Guorong Xu,Roman Sasik,Sara Brin Rosenthal,Amanda Birmingham,Sanja Coso,Gabriel Pineda,Leslie Crews,Mary E Donohoe,J Craig Venter,Thomas Whisenant,Ruben A Mesa,Ludmil B Alexandrov,Kathleen M Fisch,Catriona Jamieson
View all publications
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