AB250484

Anti-APOBEC3C/PBI antibody [EPR13417] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal APOBEC3C/PBI antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

APOBEC1L, PBI, APOBEC3C, DNA dC->dU-editing enzyme APOBEC-3C, A3C, APOBEC1-like, Phorbolin I

2 Images

Supplier Data

Western blot - Anti-APOBEC3C/PBI antibody [EPR13417] - BSA and Azide free (AB250484)

This data was developed using ab181356, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-APOBEC3C/PBI antibody [EPR13417] (<a href='/en-us/products/primary-antibodies/apobec3c-pbi-antibody-epr13417-ab181356'>ab181356</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Daudi cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 23 kDa

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Flow Cytometry (Intracellular) - Anti-APOBEC3C/PBI antibody [EPR13417] - BSA and Azide free (AB250484)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-APOBEC3C/PBI antibody [EPR13417] - BSA and Azide free (AB250484)

This data was developed using ab181356, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling APOBEC3C/PBI with ab181356 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13417

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250484 is the carrier-free version of ab181356.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC3C also known as 'PBI courier' is an enzyme that belongs to the cytidine deaminase family. This enzyme has a molecular weight of about 23 kDa. APOBEC3C functions by catalyzing the deamination of cytidine to uridine on single-stranded DNA. This process leads to mutations in viral DNA contributing to their inactivation. APOBEC3C is widely expressed in the human body with higher expression observed in immune-related tissues such as the spleen lymph nodes and peripheral blood leukocytes.

Biological function summary

APOBEC3C plays a role in the innate immune defense against viral infections. It belongs to a larger family of APOBEC3 enzymes which often work together as a complex to provide antiviral activity. The APOBEC3C enzyme contributes to the restriction of certain retroviruses and endogenous retroelements by introducing hypermutations into their genomes during replication. The presence of multiple APOBEC3 proteins within this complex suggests redundancy and diverse antiviral functions in human immunity.

Pathways

APOBEC3C is involved in the innate immune response pathway which recognizes and responds to pathogen infections including viral invasions. It interacts with other members of the APOBEC3 family such as APOBEC3G and APOBEC3B forming an elaborate network that defends the host genome from viral invasion. The enzymatic activity of APOBEC3C fits into the restriction pathway providing a protective mechanism against viral infections and maintaining genomic stability.

APOBEC3C's hypermutation activity is connected to the progression of HIV-1 where the virus may evolve escape mutants. Additionally the enzymatic action of APOBEC3C has been implicated in cancer especially in relation to the mutation profiles found in some tumor types. The interplay with other APOBEC3 proteins like APOBEC3B increases the complexity of their involvement in cancer mutagenesis and antiviral immunity highlighting the enzyme's dual role in protecting against pathogens and potentially promoting genomic instability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV), herpes simplex virus 1 (HHV-1) and Epstein-Barr virus (EBV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.

See full target information APOBEC3C

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APOBEC3C/PBI antibody [EPR13417] - BSA and Azide free

Western blot - Anti-APOBEC3C/PBI antibody [EPR13417] - BSA and Azide free (AB250484)

Flow Cytometry (Intracellular) - Anti-APOBEC3C/PBI antibody [EPR13417] - BSA and Azide free (AB250484)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise