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Rabbit Recombinant Monoclonal APOBEC3G/A3G antibody. Suitable for IP, IHC-P, WB and reacts with Human, Transfected cell lysate - Human samples.

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Images

Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926), expandable thumbnail
  • Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926), expandable thumbnail
  • Immunoprecipitation - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926), expandable thumbnail
  • Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926), expandable thumbnail
  • Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Transfected cell lysate - Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons.

    Alternative names

    DNA dC->dU-editing enzyme APOBEC-3G, APOBEC-related cytidine deaminase, APOBEC-related protein 9, CEM-15, Deoxycytidine deaminase, APOBEC-related protein, ARCD, ARP-9, CEM15, A3G, MDS019, APOBEC3G

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    Rabbit Recombinant Monoclonal APOBEC3G/A3G antibody. Suitable for IP, IHC-P, WB and reacts with Human, Transfected cell lysate - Human samples.

    Alternative names

    DNA dC->dU-editing enzyme APOBEC-3G, APOBEC-related cytidine deaminase, APOBEC-related protein 9, CEM-15, Deoxycytidine deaminase, APOBEC-related protein, ARCD, ARP-9, CEM15, A3G, MDS019, APOBEC3G

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR25404-59

    Purification technique

    Affinity purification Protein A

    Concentration
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    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    7 product images

    • Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926), expandable thumbnail

      Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST 

      This antibody does not cross-react with human APOBEC3A or APOBEC3B

      Exposure time: 180 seconds

      All lanes: Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution

      Lane 1: 293T transfected with human APOBEC3G expression vector containi a myc-His-tag®, whole cell lysate, 20 μg

      Lane 2: 293T transfected with human APOBEC3A expression vector containi a myc-His-tag®, whole cell lysate, 20 μg

      Lane 3: 293T transfected with human APOBEC3B expression vector containi a myc-His-tag®, whole cell lysate, 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 42 kDa

      Exposure time: 180s

    • Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926), expandable thumbnail

      Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST  

      Exposure time: 180 seconds

      All lanes: Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution

      Lane 1: Human tonsil tissue lysate 20 μg

      Lane 2: Human heart tissue lysate 20 μg

      Secondary

      All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Observed band size: 42 kDa

      Exposure time: 180s

    • Immunoprecipitation - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926), expandable thumbnail

      Immunoprecipitation - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926)

      APOBEC3G/A3G was immunoprecipitated from 0.35 mg H9 (human lymphoma T lymphocyte) whole cell lysate 20 ug with ab302926 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302926 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

      Lane 1: H9 whole cell lysate 20 ug

      Lane 2: ab302926 IP in H9 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab302926 in H9 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds.

      All lanes: Immunoprecipitation - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution

      Lane 1: H9 (human lymphoma T lymphocyte), whole cell lysate 20 μg

      Lane 2: H9, whole cell lysate

      Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

      Secondary

      All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

      Observed band size: 42 kDa

      Exposure time: 15s

    • Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926), expandable thumbnail

      Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Negative control: Jurkat (PMID: 14527406)

      Exposure time: 180 seconds

      All lanes: Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution

      Lane 1: H9 (human lymphoma T lymphocyte), whole cell lysate 20 μg

      Lane 2: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Observed band size: 42 kDa

      Exposure time: 180s

    • Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926), expandable thumbnail

      Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926)

      Immunohistochemical analysis of paraffin-embedded wild-type H9 cell pellet labeling APOBEC3G/A3G with ab302926 at 1/2000 (0.255 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining on wild-type H9 cell pellet (Panel A); No staining on wild-type Jurkat cell pellet (Panel B); No staining on wild-type 293T cell pellet (Panel C). The section was incubated with ab302926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926), expandable thumbnail

      Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling APOBEC3G/A3G with ab302926 at 1/2000 (0.255 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining on immune cells in human breast cancer.

      The section was incubated with ab302926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926), expandable thumbnail

      Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling APOBEC3G/A3G with ab302926 at 1/2000 (0.255 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Positive staining on immune cells in human tonsil.

      The section was incubated with ab302926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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