Anti-APOBEC3G/A3G antibody [EPR25404-59]

• IHC

Supplier Data

Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling APOBEC3G/A3G with ab302926 at 1/2000 (0.255 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on immune cells in human breast cancer. The section was incubated with ab302926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926)

Immunohistochemical analysis of paraffin-embedded wild-type H9 cell pellet labeling APOBEC3G/A3G with ab302926 at 1/2000 (0.255 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on wild-type H9 cell pellet (Panel A); No staining on wild-type Jurkat cell pellet (Panel B); No staining on wild-type 293T cell pellet (Panel C). The section was incubated with ab302926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling APOBEC3G/A3G with ab302926 at 1/2000 (0.255 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on immune cells in human tonsil. The section was incubated with ab302926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

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Immunoprecipitation - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926)

APOBEC3G/A3G was immunoprecipitated from 0.35 mg H9 (human lymphoma T lymphocyte) whole cell lysate 20 ug with ab302926 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302926 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : H9 whole cell lysate 20 ug Lane 2 : ab302926 IP in H9 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab302926 in H9 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution

Lane 1:

H9 (human lymphoma T lymphocyte), whole cell lysate 20 μg

Lane 2:

H9, whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926)

Blocking and diluting buffer and concentration : 5% NFDM/TBST   Exposure time : 180 seconds

All lanes:

Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate 20 μg

Lane 2:

Human heart tissue lysate 20 μg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 42 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926)

Blocking and diluting buffer and concentration : 5% NFDM/TBST  This antibody does not cross-react with human APOBEC3A or APOBEC3B Exposure time : 180 seconds

All lanes:

Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution

Lane 1:

293T transfected with human APOBEC3G expression vector containi a myc-His-tag®, whole cell lysate, 20 μg

Lane 2:

293T transfected with human APOBEC3A expression vector containi a myc-His-tag®, whole cell lysate, 20 μg

Lane 3:

293T transfected with human APOBEC3B expression vector containi a myc-His-tag®, whole cell lysate, 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 42 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (AB302926)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Jurkat (PMID : 14527406) Exposure time : 180 seconds

All lanes:

Western blot - Anti-APOBEC3G/A3G antibody [EPR25404-59] (ab302926) at 1/1000 dilution

Lane 1:

H9 (human lymphoma T lymphocyte), whole cell lysate 20 μg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 42 kDa

false

Exposure time: 180s