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AB280988

Anti-ApoER2 antibody [EPR23779-100] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal ApoER2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

APOER2, LRP8, Low-density lipoprotein receptor-related protein 8, LRP-8, Apolipoprotein E receptor 2

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Sandwich ELISA - Anti-ApoER2 antibody [EPR23779-100] - BSA and Azide free (Detector) (AB280988)
  • sELISA

Lab

Sandwich ELISA - Anti-ApoER2 antibody [EPR23779-100] - BSA and Azide free (Detector) (AB280988)

Sandwich ELISA of ab253578 with the capture antibody ab281138 dilution at 2 µg/mL and detector antibody ab280988 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23779-100

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab280988 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LRP8. The recommended pair for sandwich ELISA is:
Capture: ab281138, Human LRP8 Capture Antibody (unconjugated)
Detector: ab280988, Human LRP8 Detector Antibody (unconjugated)
The reference range value is 15.63 - 4000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ApoER2 also known as apolipoprotein E receptor 2 or LRP8 is a member of the LDL receptor family weighing about 130 kDa. It is expressed mainly in the brain but also in the heart and other tissues. This receptor interacts with extracellular binding partners such as lipoproteins and reelin to mediate signal transduction across cell membranes. ApoER2 plays an important role in brain development and synaptic plasticity through these interactions.
Biological function summary

ApoER2 contributes to neural processes like synaptic transmission and neuronal migration. It forms part of a protein complex with reelin a process essential for dendritic spine development and long-term potentiation. Its involvement in transporting cholesterol and other lipids is critical for maintaining neuronal function and integrity.

Pathways

ApoER2 participates in the reelin signaling pathway which is important for brain layer formation during development. It also interacts with other proteins like Disabled-1 (Dab1) to propagate signals initiated by reelin binding. This pathway also links ApoER2 to the regulation of synaptic strength and plasticity which are significant in learning and memory processes.

ApoER2 has connections to Alzheimer's disease and schizophrenia. Its interaction with amyloid-beta precursor protein (APP) in Alzheimer's disease impacts plaque formation and neuronal cell loss. In schizophrenia altered reelin-ApoER2 signaling can disrupt synaptic functions suggesting its involvement in the disorder's pathophysiology. Understanding these interactions may offer insights into potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands (PubMed : 12899622, PubMed : 12950167, PubMed : 20223215, PubMed : 30873003). LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail (By similarity). Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons (By similarity). LRP8 has higher affinity for Reelin than VLDLR (By similarity). LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development (By similarity). Binds the endoplasmic reticulum resident receptor-associated protein (RAP) (By similarity). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature (PubMed : 12807892). Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation (By similarity). May also function as an endocytic receptor (By similarity). Not required for endocytic uptake of SEPP1 in the kidney which is mediated by LRP2 (By similarity). Together with its ligand, apolipoprotein E (apoE), may indirectly play a role in the suppression of the innate immune response by controlling the survival of myeloid-derived suppressor cells (By similarity).. (Microbial infection) Acts as a receptor for Semliki Forest virus.
See full target information LRP8
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