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AB108208

Anti-ApoER2 antibody [EPR3326]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

4

(2 Reviews)

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(34 Publications)

Anti-ApoER2 antibody [EPR3326] (ab108208) is a rabbit monoclonal antibody detecting ApoER2 in Western Blot, IP. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

APOER2, LRP8, Low-density lipoprotein receptor-related protein 8, LRP-8, Apolipoprotein E receptor 2

4 Images
Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (AB108208)
  • IP

Unknown

Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (AB108208)

ab108208 (purified) at 1/40 immunoprecipitating ApoER2 in SH-SY5Y (human neuroblastoma) whole cell lysate.

Lane 1 (input) : SH-SY5Y whole cell lysate 10ug

Lane 2 (+) : ab108208 + SH-SY5Y whole cell lysate

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108208 in SH-SY5Y whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (ab108208)

Predicted band size: 106 kDa

Observed band size: 130-180 kDa

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Western blot - Anti-ApoER2 antibody [EPR3326] (AB108208)
  • WB

Lab

Western blot - Anti-ApoER2 antibody [EPR3326] (AB108208)

Lanes 1 - 2 : Merged signal (red and green). Green - ab108208 observed at 106 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab108208 was shown to specifically react with ApoER2 in wild-type HAP1 cells as signal was lost in LRP8 knockout cells. Wild-type and ApoER2 knockout samples were subjected to SDS-PAGE. ab108208 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 40 µg

Lane 2:

ApoER2 knockout HAP1 whole cell lysate at 40 µg

Predicted band size: 106 kDa

false

Western blot - Anti-ApoER2 antibody [EPR3326] (AB108208)
  • WB

Unknown

Western blot - Anti-ApoER2 antibody [EPR3326] (AB108208)

Blocking and diluting buffer 5% NFDM/TBST.

130kDa : Immature form;

170kDa : Glycosylated mature form. (PMID : 24344333, 24532792, 24705369 and 25233900)

All lanes:

Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/10000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma) whole cell lysate at 20 µg

Lane 2:

Human fetal brain tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

C6 (rat glioma) whole cell lysate at 20 µg

Lane 5:

Neuro-2a (mouse neuroblastoma) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 106 kDa

Observed band size: 130-170 kDa

false

Western blot - Anti-ApoER2 antibody [EPR3326] (AB108208)
  • WB

Unknown

Western blot - Anti-ApoER2 antibody [EPR3326] (AB108208)

All lanes:

Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

C6 cell lysate at 10 µg

Lane 3:

Neuro-2a cell lysate at 10 µg

Lane 4:

Human Fetal Brain cell lysate at 10 µg

Lane 5:

Mouse Fetal Brain cell lysate at 10 µg

Lane 6:

Rat Fetal Brain cell lysate at 10 µg

Predicted band size: 106 kDa

false

  • Carrier free

    Anti-ApoER2 antibody [EPR3326] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3326

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-ApoER2 antibody [EPR3326] (ab108208) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.

What is the molecular weight of ApoER2?
Anti-ApoER2 [EPR3326] (ab108208) specifically detects a band for ApoER2 (UniProt: Q14114) at a molecular weight of 106kDa.

Trusted by the scientific community
Anti-ApoER2 [EPR3326] (ab108208) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-ApoER2 antibody [EPR3326] (ab108208) has been confirmed by Western blot testing in LRP8 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR3326] also available for your convenience: ab108208, Carrier free - ab215274

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ApoER2 also known as apolipoprotein E receptor 2 or LRP8 is a member of the LDL receptor family weighing about 130 kDa. It is expressed mainly in the brain but also in the heart and other tissues. This receptor interacts with extracellular binding partners such as lipoproteins and reelin to mediate signal transduction across cell membranes. ApoER2 plays an important role in brain development and synaptic plasticity through these interactions.
Biological function summary

ApoER2 contributes to neural processes like synaptic transmission and neuronal migration. It forms part of a protein complex with reelin a process essential for dendritic spine development and long-term potentiation. Its involvement in transporting cholesterol and other lipids is critical for maintaining neuronal function and integrity.

Pathways

ApoER2 participates in the reelin signaling pathway which is important for brain layer formation during development. It also interacts with other proteins like Disabled-1 (Dab1) to propagate signals initiated by reelin binding. This pathway also links ApoER2 to the regulation of synaptic strength and plasticity which are significant in learning and memory processes.

ApoER2 has connections to Alzheimer's disease and schizophrenia. Its interaction with amyloid-beta precursor protein (APP) in Alzheimer's disease impacts plaque formation and neuronal cell loss. In schizophrenia altered reelin-ApoER2 signaling can disrupt synaptic functions suggesting its involvement in the disorder's pathophysiology. Understanding these interactions may offer insights into potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands (PubMed : 12899622, PubMed : 12950167, PubMed : 20223215, PubMed : 30873003). LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail (By similarity). Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons (By similarity). LRP8 has higher affinity for Reelin than VLDLR (By similarity). LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development (By similarity). Binds the endoplasmic reticulum resident receptor-associated protein (RAP) (By similarity). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature (PubMed : 12807892). Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation (By similarity). May also function as an endocytic receptor (By similarity). Not required for endocytic uptake of SEPP1 in the kidney which is mediated by LRP2 (By similarity). Together with its ligand, apolipoprotein E (apoE), may indirectly play a role in the suppression of the innate immune response by controlling the survival of myeloid-derived suppressor cells (By similarity).. (Microbial infection) Acts as a receptor for Semliki Forest virus.
See full target information LRP8
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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 101:102238 PubMed40907945

2025

Apolipoprotein E receptor 2 in endothelium promote glucose tolerance by mediating insulin delivery to skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasia Sacharidou,Ken L Chambliss,Jun Peng,Keiji Tanigaki,Md Nurul Islam,Shashank Sirsi,Andrew Lemoff,Kenneth Hoyt,Chieko Mineo,Philip W Shaul

Frontiers in cellular neuroscience 18:1492048 PubMed39600307

2024

Transcriptome analyses reveal molecular mechanisms of novel compound heterozygous variants causing infantile cerebellar retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Wenke Yang,Shuyue Wang,Ke Yang,Yanjun Li,Zhenglong Guo,Jianmei Huang,Jinming Wang,Shixiu Liao

EMBO molecular medicine 16:2749-2774 PubMed39433871

2024

Breast cancer secretes anti-ferroptotic MUFAs and depends on selenoprotein synthesis for metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Ackermann,Engy Shokry,Ruhi Deshmukh,Jayanthi Anand,Laura C A Galbraith,Louise Mitchell,Giovanny Rodriguez-Blanco,Victor H Villar,Britt Amber Sterken,Colin Nixon,Sara Zanivan,Karen Blyth,David Sumpton,Saverio Tardito

PLoS genetics 20:e1011348 PubMed39038048

2024

APOER2 splicing repertoire in Alzheimer's disease: Insights from long-read RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Christina M Gallo,Sabrina A Kistler,Anna Natrakul,Adam T Labadorf,Uwe Beffert,Angela Ho

Nature structural & molecular biology 31:1277-1285 PubMed38867112

2024

PRDX6 augments selenium utilization to limit iron toxicity and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Fujita,Yu-Ki Tanaka,Seiryo Ogata,Noriyuki Suzuki,Sota Kuno,Uladzimir Barayeu,Takaaki Akaike,Yasumitsu Ogra,Kazuhiro Iwai

The Journal of biological chemistry 299:105009 PubMed37406814

2023

An efficient selenium transport pathway of selenoprotein P utilizing a high-affinity ApoER2 receptor variant and being independent of selenocysteine lyase.

Applications

Unspecified application

Species

Unspecified reactive species

Ayako Mizuno,Takashi Toyama,Atsuya Ichikawa,Naoko Sakai,Yuya Yoshioka,Yukina Nishito,Renya Toga,Hiroshi Amesaka,Takayuki Kaneko,Kotoko Arisawa,Ryouhei Tsutsumi,Yuichiro Mita,Shun-Ichi Tanaka,Noriko Noguchi,Yoshiro Saito

Journal of virology 97:e0075823 PubMed37338407

2023

Liver X Receptor-Inducible Host E3 Ligase IDOL Targets a Human Cytomegalovirus Reactivation Determinant.

Applications

Unspecified application

Species

Unspecified reactive species

Luwanika Mlera,Donna Collins-McMillen,Sebastian Zeltzer,Jason C Buehler,Melissa Moy,Kristen Zarrella,Katie Caviness,Louis Cicchini,David J Tafoya,Felicia Goodrum

Nature communications 14:3479 PubMed37311819

2023

Excessive copper impairs intrahepatocyte trafficking and secretion of selenoprotein P.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Schwarz,Caroline E Meyer,Alina Löser,Kristina Lossow,Julian Hackler,Christiane Ott,Susanne Jäger,Isabelle Mohr,Ella A Eklund,Angana A H Patel,Nadia Gul,Samantha Alvarez,Ilayda Altinonder,Clotilde Wiel,Maria Maares,Hajo Haase,Anetta Härtlova,Tilman Grune,Matthias B Schulze,Tanja Schwerdtle,Uta Merle,Hans Zischka,Volkan I Sayin,Lutz Schomburg,Anna P Kipp

American journal of physiology. Regulatory, integrative and comparative physiology 325:R55-R68 PubMed37212552

2023

Different sensitivity to diet-induced hyperinsulinemia and hyperglycemia between mice with global or bone marrow-specific apoE receptor-2 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick R Wolfkiel,April M Haller,Jillian Kirby,Anja Jaeschke,David Y Hui

Scientific reports 13:431 PubMed36624108

2023

Apolipoprotein C3 facilitates internalization of cationic lipid nanoparticles into bone marrow-derived mouse mast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Syed Benazir Alam,Feng Wang,Hui Qian,Marianna Kulka
View all publications
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