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AB231585

Anti-APOF antibody

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(3 Publications)

Rabbit Polyclonal APOF antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Apolipoprotein F aa 100 to C-terminus.

View Alternative Names

Apolipoprotein F, Apo-F, Lipid transfer inhibitor protein, LTIP, APOF

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOF antibody (AB231585)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOF antibody (AB231585)

Paraffin-embedded human esophagus tissue stained for APOF using ab231585 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOF antibody (AB231585)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOF antibody (AB231585)

Formalin-fixed, paraffin-embedded human liver tissue stained for APOF using ab231585 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-APOF antibody (AB231585)
  • WB

Supplier Data

Western blot - Anti-APOF antibody (AB231585)

All lanes:

Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL

All lanes:

Human liver lysate.

Predicted band size: 35 kDa

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Western blot - Anti-APOF antibody (AB231585)
  • WB

Supplier Data

Western blot - Anti-APOF antibody (AB231585)

All lanes:

Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL

All lanes:

Mouse liver lysate.

Predicted band size: 35 kDa

false

Western blot - Anti-APOF antibody (AB231585)
  • WB

Supplier Data

Western blot - Anti-APOF antibody (AB231585)

All lanes:

Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL

All lanes:

Human lung lysate.

Predicted band size: 35 kDa

false

Western blot - Anti-APOF antibody (AB231585)
  • WB

Supplier Data

Western blot - Anti-APOF antibody (AB231585)

All lanes:

Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL

All lanes:

Mouse cerebrum lysate.

Predicted band size: 35 kDa

false

Western blot - Anti-APOF antibody (AB231585)
  • WB

Supplier Data

Western blot - Anti-APOF antibody (AB231585)

All lanes:

Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL

All lanes:

Recombinant human APOF protein

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Apolipoprotein F aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13790

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231585 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The APOF protein also known as Apolipoprotein F serves a significant role in lipid metabolism. This protein has a molecular weight of approximately 29 kDa. APOF is expressed mainly in the liver and is found in the blood plasma. It is part of the lipoprotein system where it associates with lipoproteins as it circulates within the bloodstream.
Biological function summary

The APOF protein helps regulate lipid transport and cholesterol ester uptake. It is involved in the modulation of the plasma lipoprotein levels important for maintaining lipid homeostasis. APOF does not work independently but interacts with other lipoproteins in the plasma to regulate lipid transfer processes. Its control over cholesterol homeostasis hints at its involvement in complex lipoprotein metabolic pathways.

Pathways

APOF plays a part in the reverse cholesterol transport pathways. This pathway is essential for the maintenance of cholesterol balance in the body. APOF affects the function of very low-density lipoproteins (VLDL) and is related to other proteins like apolipoprotein A1 which also contribute to cholesterol transport and lipoprotein metabolism.

An imbalance or alteration in APOF levels is linked to hyperlipoproteinemia and cardiovascular diseases. These conditions are associated with cholesterol and triglyceride metabolism where APOF and related proteins like low-density lipoprotein (LDL) play significant roles. The modulation of APOF activity presents a potential target for therapeutic strategies in managing lipid-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
See full target information Apolipoprotein F
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 91:102069 PubMed39571901

2024

The immune checkpoint molecule B7-H4 regulates β-cell mass and insulin secretion by modulating cholesterol metabolism through Stat5 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Fangzhen Xia,Ziteng Zhang,Zhen Qian,Xiaoyu Fang,Junxue Wang,Yan Wang,Guoting Sun,Yuefeng Yu,Ninjian Wang,Junke Zhen,Yan Liu,Yingli Lu

NPJ Parkinson's disease 10:194 PubMed39443520

2024

CRBN modulates synuclein fibrillation via degradation of DNAJB1 in mouse model of Parkinson disease.

Applications

Unspecified application

Species

Unspecified reactive species

Uroos Akber,Jun-Hyung Jung,Heewoong Yoon,Jiwon Seo,Chul-Seung Park

Communications biology 4:357 PubMed33742113

2021

β-catenin-promoted cholesterol metabolism protects against cellular senescence in naked mole-rat cells.

Applications

Unspecified application

Species

Unspecified reactive species

Woei-Yaw Chee,Yuriko Kurahashi,Junhyeong Kim,Kyoko Miura,Daisuke Okuzaki,Tohru Ishitani,Kentaro Kajiwara,Shigeyuki Nada,Hideyuki Okano,Masato Okada
View all publications
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