Rabbit Polyclonal APOF antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Apolipoprotein F aa 100 to C-terminus.
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- Communications biology 4:3572021β-catenin-promoted cholesterol metabolism protects against cellular senescence in naked mole-rat cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWoei-Yaw Chee,Yuriko Kurahashi,Junhyeong Kim,Kyoko Miura,Daisuke Okuzaki,Tohru Ishitani,Kentaro Kajiwara,Shigeyuki Nada,Hideyuki Okano,Masato OkadaPubMed 33742113
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Apolipoprotein F aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13790
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.20000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.20000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
Alternative names
Apolipoprotein F, Apo-F, Lipid transfer inhibitor protein, LTIP, APOF
Recommended products
Rabbit Polyclonal APOF antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Apolipoprotein F aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Apolipoprotein F aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13790
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab231585 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The APOF protein also known as Apolipoprotein F serves a significant role in lipid metabolism. This protein has a molecular weight of approximately 29 kDa. APOF is expressed mainly in the liver and is found in the blood plasma. It is part of the lipoprotein system where it associates with lipoproteins as it circulates within the bloodstream.
- Biological function summary
The APOF protein helps regulate lipid transport and cholesterol ester uptake. It is involved in the modulation of the plasma lipoprotein levels important for maintaining lipid homeostasis. APOF does not work independently but interacts with other lipoproteins in the plasma to regulate lipid transfer processes. Its control over cholesterol homeostasis hints at its involvement in complex lipoprotein metabolic pathways.
- Pathways
APOF plays a part in the reverse cholesterol transport pathways. This pathway is essential for the maintenance of cholesterol balance in the body. APOF affects the function of very low-density lipoproteins (VLDL) and is related to other proteins like apolipoprotein A1 which also contribute to cholesterol transport and lipoprotein metabolism.
- Associated diseases and disorders
An imbalance or alteration in APOF levels is linked to hyperlipoproteinemia and cardiovascular diseases. These conditions are associated with cholesterol and triglyceride metabolism where APOF and related proteins like low-density lipoprotein (LDL) play significant roles. The modulation of APOF activity presents a potential target for therapeutic strategies in managing lipid-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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7 product images
Western blot - Anti-APOF antibody (ab231585)
All lanes: Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL
All lanes: Mouse liver lysate.
Predicted band size: 35 kDa
Western blot - Anti-APOF antibody (ab231585)
All lanes: Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL
All lanes: Human lung lysate.
Predicted band size: 35 kDa
Western blot - Anti-APOF antibody (ab231585)
All lanes: Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL
All lanes: Human liver lysate.
Predicted band size: 35 kDa
Western blot - Anti-APOF antibody (ab231585)
All lanes: Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL
All lanes: Mouse cerebrum lysate.
Predicted band size: 35 kDa
Western blot - Anti-APOF antibody (ab231585)
All lanes: Western blot - Anti-APOF antibody (ab231585) at 2 µg/mL
All lanes: Recombinant human APOF protein
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOF antibody (ab231585)
Paraffin-embedded human esophagus tissue stained for APOF using ab231585 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOF antibody (ab231585)
Formalin-fixed, paraffin-embedded human liver tissue stained for APOF using ab231585 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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