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AB284802

Anti-APOF antibody [EP2909] - BSA and Azide free

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Rabbit Monoclonal APOF antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Apolipoprotein F, Apo-F, Lipid transfer inhibitor protein, LTIP, APOF

1 Images
Western blot - Anti-APOF antibody [EP2909] - BSA and Azide free (AB284802)
  • WB

Unknown

Western blot - Anti-APOF antibody [EP2909] - BSA and Azide free (AB284802)

This data was developed using ab133295, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-APOF antibody [EP2909] (<a href='/en-us/products/primary-antibodies/apof-antibody-ep2909-ab133295'>ab133295</a>) at 1/1000 dilution

All lanes:

Human plasma cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2909

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284802 is the carrier-free version of ab133295

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The APOF protein also known as Apolipoprotein F serves a significant role in lipid metabolism. This protein has a molecular weight of approximately 29 kDa. APOF is expressed mainly in the liver and is found in the blood plasma. It is part of the lipoprotein system where it associates with lipoproteins as it circulates within the bloodstream.
Biological function summary

The APOF protein helps regulate lipid transport and cholesterol ester uptake. It is involved in the modulation of the plasma lipoprotein levels important for maintaining lipid homeostasis. APOF does not work independently but interacts with other lipoproteins in the plasma to regulate lipid transfer processes. Its control over cholesterol homeostasis hints at its involvement in complex lipoprotein metabolic pathways.

Pathways

APOF plays a part in the reverse cholesterol transport pathways. This pathway is essential for the maintenance of cholesterol balance in the body. APOF affects the function of very low-density lipoproteins (VLDL) and is related to other proteins like apolipoprotein A1 which also contribute to cholesterol transport and lipoprotein metabolism.

An imbalance or alteration in APOF levels is linked to hyperlipoproteinemia and cardiovascular diseases. These conditions are associated with cholesterol and triglyceride metabolism where APOF and related proteins like low-density lipoprotein (LDL) play significant roles. The modulation of APOF activity presents a potential target for therapeutic strategies in managing lipid-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
See full target information Apolipoprotein F
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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