AB232035

Anti-APOF antibody [EPR2908] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal APOF antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Apolipoprotein F, Apo-F, Lipid transfer inhibitor protein, LTIP, APOF

1 Images

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Western blot - Anti-APOF antibody [EPR2908] - BSA and Azide free (AB232035)

This WB data was generated using the same anti-Apolipoprotein F antibody clone, EPR2908, in a different buffer formulation (cat# ab92301).

All lanes:

Western blot - Anti-APOF antibody [EPR2908] - BSA and Azide free (ab232035) at 1/2000 dilution

Lane 1:

HDL lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2908

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab232035 is the carrier-free version of ab92301.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The APOF protein also known as Apolipoprotein F serves a significant role in lipid metabolism. This protein has a molecular weight of approximately 29 kDa. APOF is expressed mainly in the liver and is found in the blood plasma. It is part of the lipoprotein system where it associates with lipoproteins as it circulates within the bloodstream.

Biological function summary

The APOF protein helps regulate lipid transport and cholesterol ester uptake. It is involved in the modulation of the plasma lipoprotein levels important for maintaining lipid homeostasis. APOF does not work independently but interacts with other lipoproteins in the plasma to regulate lipid transfer processes. Its control over cholesterol homeostasis hints at its involvement in complex lipoprotein metabolic pathways.

Pathways

APOF plays a part in the reverse cholesterol transport pathways. This pathway is essential for the maintenance of cholesterol balance in the body. APOF affects the function of very low-density lipoproteins (VLDL) and is related to other proteins like apolipoprotein A1 which also contribute to cholesterol transport and lipoprotein metabolism.

An imbalance or alteration in APOF levels is linked to hyperlipoproteinemia and cardiovascular diseases. These conditions are associated with cholesterol and triglyceride metabolism where APOF and related proteins like low-density lipoprotein (LDL) play significant roles. The modulation of APOF activity presents a potential target for therapeutic strategies in managing lipid-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.

See full target information Apolipoprotein F

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 10:2523-2538 PubMed34295659

2021

One carbon metabolism in human lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Yao,Luogen Peng,Omar Elakad,Stefan Küffer,Marc Hinterthaner,Bernhard C Danner,Alexander von Hammerstein-Equord,Philipp Ströbel,Hanibal Bohnenberger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APOF antibody [EPR2908] - BSA and Azide free

Western blot - Anti-APOF antibody [EPR2908] - BSA and Azide free (AB232035)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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