Rabbit Recombinant Monoclonal APOL3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-APOL3 antibody [EPR8238(2)] (AB154869), expandable thumbnail](https://content.abcam.com/products/images/apol3-antibody-epr82382-ab154869--western-blot-img105680.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-APOL3 antibody [EPR8238(2)] (AB154869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (AB154869), expandable thumbnail](https://content.abcam.com/products/images/apol3-antibody-epr82382-ab154869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123103.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (AB154869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (AB154869), expandable thumbnail](https://content.abcam.com/products/images/apol3-antibody-epr82382-ab154869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71138.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (AB154869)")
Publications
Filter by
- Cell 186:621-645.e332023Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJérémie Rosain,Anna-Lena Neehus,Jérémy Manry,Rui Yang,Jérémie Le Pen,Wassim Daher,Zhiyong Liu,Yi-Hao Chan,Natalia Tahuil,Özden Türel,Mathieu Bourgey,Masato Ogishi,Jean-Marc Doisne,Helena M Izquierdo,Takayoshi Shirasaki,Tom Le Voyer,Antoine Guérin,Paul Bastard,Marcela Moncada-Vélez,Ji Eun Han,Taushif Khan,Franck Rapaport,Seon-Hui Hong,Andrew Cheung,Kathrin Haake,Barbara C Mindt,Laura Pérez,Quentin Philippot,Danyel Lee,Peng Zhang,Darawan Rinchai,Fatima Al Ali,Manar Mahmoud Ahmad Ata,Mahbuba Rahman,Jessica N Peel,Søren Heissel,Henrik Molina,Yasemin Kendir-Demirkol,Rasheed Bailey,Shuxiang Zhao,Jonathan Bohlen,Mathieu Mancini,Yoann Seeleuthner,Marie Roelens,Lazaro Lorenzo,Camille Soudée,María Elvira Josefina Paz,María Laura González,Mohamed Jeljeli,Jean Soulier,Serge Romana,Anne-Sophie L'Honneur,Marie Materna,Rubén Martínez-Barricarte,Mathieu Pochon,Carmen Oleaga-Quintas,Alexandre Michev,Mélanie Migaud,Romain Lévy,Marie-Alexandra Alyanakian,Flore Rozenberg,Carys A Croft,Guillaume Vogt,Jean-François Emile,Laurent Kremer,Cindy S Ma,Jörg H Fritz,Stanley M Lemon,András N Spaan,Nicolas Manel,Laurent Abel,Margaret R MacDonald,Stéphanie Boisson-Dupuis,Nico Marr,Stuart G Tangye,James P Di Santo,Qian Zhang,Shen-Ying Zhang,Charles M Rice,Vivien Béziat,Nico Lachmann,David Langlais,Jean-Laurent Casanova,Philippe Gros,Jacinta BustamantePubMed 36736301
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles.
Alternative names
Apolipoprotein L3, Apolipoprotein L-III, TNF-inducible protein CG12-1, ApoL-III, CG12_1, APOL3
Recommended products
Rabbit Recombinant Monoclonal APOL3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8238(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
APOL3 also known as Apolipoprotein L3 is a protein with a mass of approximately 40 kDa. It belongs to the apolipoprotein family which plays a role in lipid metabolism. APOL3 is expressed mainly in vascular tissues and immune system cells. Its primary mechanical function involves lipid transportation and regulation within cellular compartments contributing to maintaining cellular lipid homeostasis.
- Biological function summary
APOL3 operates as part of a complex involved in cellular lipid transport processes. This protein participates in trafficking lipids within cellular membranes and organelles impacting cell membrane dynamics. APOL3 gene expression varies in different tissues influencing how cells manage and utilize lipid molecules. It associates with cellular stress responses especially during pathogen invasions aiding immune system efficiency.
- Pathways
APOL3 plays a role in lipid metabolism pathways and immune regulatory pathways. The protein interacts with components of these pathways such as apolipoprotein L family members and enzymes involved in lipid processing. Its activity in these pathways modulates lipid transport and immune responses interacting with components like cholesterol transport proteins and immune receptors. APOL3 contributes to maintaining lipid balance and promoting cellular defense mechanisms.
- Associated diseases and disorders
APOL3 shows a connection to cardiovascular diseases and autoimmune disorders. Abnormalities in its expression can contribute to the lipid dysfunctions associated with atherosclerosis. It also relates to the immune dysregulation observed in certain autoimmune conditions. APOL3 interacts with other apolipoproteins and immune regulatory proteins influencing disease progression and severity. Understanding its role in these contexts can guide therapeutic strategies targeting lipid and immune system abnormalities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-APOL3 antibody [EPR8238(2)] (ab154869), expandable thumbnail](https://content.abcam.com/products/images/apol3-antibody-epr82382-ab154869--western-blot-img105680.jpg "Western blot - Anti-APOL3 antibody [EPR8238(2)] (ab154869)")
Western blot - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
All lanes: Western blot - Anti-APOL3 antibody [EPR8238(2)] (ab154869) at 1/1000 dilution
Lane 1: Fetal kidney tissue lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: Daudi cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869), expandable thumbnail](https://content.abcam.com/products/images/apol3-antibody-epr82382-ab154869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123103.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling APOL3 with ab154869 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869), expandable thumbnail](https://content.abcam.com/products/images/apol3-antibody-epr82382-ab154869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71138.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling APOL3 with ab154869 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
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- Visit the general protocols
- Visit troubleshooting
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