Anti-Apolipoprotein A I antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody (AB227455)

Immunofluorescence analysis of HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Apolipoprotein A1 protein at Golgi apparatus using ab227455 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).
Scale bar= 10 µm.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody (AB227455)

Immunohistochemical analysis of paraffin-embedded human colon tissue stained for secreted Apolipoprotein A1 protein with ab227455 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody (AB227455)

Immunofluorescence analysis of HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Apolipoprotein A1 protein at cytoplasm using ab227455 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

• WB

Supplier Data

Western blot - Anti-Apolipoprotein A I antibody (AB227455)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Apolipoprotein A I antibody (ab227455) at 1/10000 dilution

All lanes:

Human plasma at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 31 kDa

false

• WB

Supplier Data

Western blot - Anti-Apolipoprotein A I antibody (AB227455)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Apolipoprotein A I antibody (ab227455) at 1/10000 dilution

Lane 1:

Mouse plasma at 30 µg

Lane 2:

Rat plasma at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 31 kDa

false