AB7613

Anti-Apolipoprotein A I antibody

Name: Anti-Apolipoprotein A I antibody (ab7613) | Abcam
Brand: Abcam Limited
SKU: AB7613
Price: 535 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(42 Publications)

Goat Polyclonal Apolipoprotein A I antibody. Suitable for IP, ELISA, WB, IHC-P and reacts with Human samples. Cited in 42 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human APOA1.

View Alternative Names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ELISA, IHC-P, WB

applications

Immunogen

Native Full Length Protein corresponding to Human APOA1.

P02647

Specificity

Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/20000", "ELISA-species-notes": "Can be paired with <a href='/en-us/products/primary-antibodies/hrp-apolipoprotein-a-i-antibody-ab20784'>ab20784</a> for sandwich ELISA.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" } } }

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Product details

This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Storage buffer

pH: 8 Preservative: 0.01% Sodium azide Constituents: 4.77% Sodium borate, 0.44% Sodium chloride, 0.15% EDTA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.

Biological function summary

ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.

Pathways

The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.

Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

See full target information APOA1

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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39941151

2025

Differential Regulation of miRNA and Protein Profiles in Human Plasma-Derived Extracellular Vesicles via Continuous Aerobic and High-Intensity Interval Training.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenghao Wang,Yiran Ou,Xinyue Zhu,Ye Zhou,Xiaowei Zheng,Meixia Zhang,Sheyu Li,Shao-Nian Yang,Lisa Juntti-Berggren,Per-Olof Berggren,Xiaofeng Zheng

Journal of the American Heart Association 12:e030476 PubMed37889183

2023

ANGPTL3 (Angiopoietin-Like 3) Preferentially Resides on High-Density Lipoprotein in the Human Circulation, Affecting Its Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan M Kraaijenhof,Tycho R Tromp,Nick S Nurmohamed,Laurens F Reeskamp,Marije Langenkamp,Johannes H M Levels,S Matthijs Boekholdt,Nicholas J Wareham,Menno Hoekstra,Erik S G Stroes,G Kees Hovingh,Aldo Grefhorst

Heart and vessels 37:161-171 PubMed34459957

2021

Anti-inflammatory HDL effects are impaired in atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Holzwirth,Tina Fischer-Schaepmann,Danilo Obradovic,Mirjam von Lucadou,Edzard Schwedhelm,Günter Daum,Gerhard Hindricks,Gunther Marsche,Markus Trieb,Holger Thiele,Jelena Kornej,Petra Büttner

Journal of extracellular vesicles 10:e12044 PubMed33489012

2021

Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Dong,Richard C Zieren,Kengo Horie,Chi-Ju Kim,Emily Mallick,Yuezhou Jing,Mingxiao Feng,Morgan D Kuczler,Jordan Green,Sarah R Amend,Kenneth W Witwer,Theo M de Reijke,Yoon-Kyoung Cho,Kenneth J Pienta,Wei Xue

Internal medicine (Tokyo, Japan) 60:575-581 PubMed32999221

2020

Giant Hepatomegaly with Spleno-testicular Enlargement in a Patient with Apolipoprotein A-I Amyloidosis: An Uncommon Type of Amyloidosis in Japan.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuneaki Yoshinaga,Nagaaki Katoh,Masahide Yazaki,Mitsuto Sato,Fuyuki Kametani,Hideo Yasuda,Kyohei Watanabe,Kazuhito Kawata,Mayuko Nakagawa,Yoshiki Sekijima

Malaria journal 19:155 PubMed32295584

2020

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Tougan,Jyotheeswara R Edula,Masayuki Morita,Eizo Takashima,Hajime Honma,Takafumi Tsuboi,Toshihiro Horii

Investigative ophthalmology & visual science 61:6 PubMed32031578

2020

TNFα-Mediated Priming of Mesenchymal Stem Cells Enhances Their Neuroprotective Effect on Retinal Ganglion Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ben Mead,Xitiz Chamling,Donald J Zack,Zubair Ahmed,Stanislav Tomarev

EBioMedicine 43:114-126 PubMed31047861

2019

Ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse extracellular vesicles from blood.

Applications

Unspecified application

Species

Unspecified reactive species

Michela Notarangelo,Chiara Zucal,Angelika Modelska,Isabella Pesce,Giorgina Scarduelli,Cristina Potrich,Lorenzo Lunelli,Cecilia Pederzolli,Paola Pavan,Giancarlo la Marca,Luigi Pasini,Paola Ulivi,Himisha Beltran,Francesca Demichelis,Alessandro Provenzani,Alessandro Quattrone,Vito G D'Agostino

EBioMedicine 43:43-53 PubMed30982767

2019

APOA1 oxidation is associated to dysfunctional high-density lipoproteins in human abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Diego Martínez-López,Emilio Camafeita,Lídia Cedó,Raquel Roldan-Montero,Inmaculada Jorge,Fernando García-Marqués,María Gómez-Serrano,Elena Bonzon-Kulichenko,Francisco Blanco-Vaca,Luis Miguel Blanco-Colio,Jean-Baptiste Michel,Joan Carles Escola-Gil,Jesús Vázquez,Jose Luis Martin-Ventura

Investigative ophthalmology & visual science 59:5473-5480 PubMed30452601

2018

Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Promote Neuroprotection in a Genetic DBA/2J Mouse Model of Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ben Mead,Zubair Ahmed,Stanislav Tomarev

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apolipoprotein A I antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (42)

Product promise