AB7614

Anti-Apolipoprotein A I antibody

(5 Reviews)

(17 Publications)

Goat Polyclonal Apolipoprotein A I antibody. Suitable for IP, ELISA, WB, sELISA, IHC-P and reacts with Mouse samples. Cited in 17 publications. Immunogen corresponding to Native Full Length Protein corresponding to Mouse Apoa1.

View Alternative Names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, Apoa1

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB, ELISA, IP, sELISA

applications

Immunogen

Native Full Length Protein corresponding to Mouse Apoa1.

Q00623

Specificity

Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other mouse serum proteins is negligible.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 4.77% Sodium borate, 0.44% Sodium chloride, 0.15% EDTA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.

Biological function summary

ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.

Pathways

The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.

Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

See full target information Apoa1

style

left-column

Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3752 PubMed40263360

2025

Apolipoprotein-A1 transports and regulates MMP2 in the blood.

Applications

Unspecified application

Species

Unspecified reactive species

Hassan Sarker,Rashmi Panigrahi,Ana Lopez-Campistrous,Todd McMullen,Ken Reyes,Elena Anderson,Vidhya Krishnan,Samuel Hernandez-Anzaldo,Xi-Long Zheng,J N Mark Glover,Eugenio Hardy,Carlos Fernandez-Patron

Microbiology and immunology 65:438-448 PubMed34270107

2021

Validation of serum apolipoprotein A1 in rabies virus-infected mice as a biomarker for the preclinical diagnosis of rabies.

Applications

Unspecified application

Species

Unspecified reactive species

Kentaro Yamada,Koji Kuribayashi,Naotaka Inomata,Kazuko Noguchi,Kazunori Kimitsuki,Catalino S Demetria,Nobuo Saito,Satoshi Inoue,Chun-Ho Park,Ryo Kaimori,Motoi Suzuki,Mariko Saito-Obata,Yasuhiko Kamiya,Daria L Manalo,Beatriz P Quiambao,Akira Nishizono

Frontiers in genetics 11:565479 PubMed33250918

2020

Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqi Song,Yanfeng Xu,Ling Zhang,Li Zhou,Yu Zhang,Yunlin Han,Xianglei Li,Pin Yu,Yajin Qu,Wenjie Zhao,Chuan Qin

Cell chemical biology 27:1130-1139.e4 PubMed32402239

2020

A Plasma Protein Network Regulates PM20D1 and N-Acyl Amino Acid Bioactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Joon T Kim,Mark P Jedrychowski,Wei Wei,Daniel Fernandez,Curt R Fischer,Steven M Banik,Bruce M Spiegelman,Jonathan Z Long

Hepatology (Baltimore, Md.) 70:1972-1985 PubMed31081165

2019

Hepatocyte Deletion of Triglyceride-Synthesis Enzyme Acyl CoA: Diacylglycerol Acyltransferase 2 Reduces Steatosis Without Increasing Inflammation or Fibrosis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nina L Gluchowski,Katlyn R Gabriel,Chandramohan Chitraju,Roderick T Bronson,Niklas Mejhert,Sebastian Boland,Kun Wang,Zon Weng Lai,Robert V Farese,Tobias C Walther

Journal of translational medicine 16:155 PubMed29879989

2018

Protein markers of dysfunctional HDL in scavenger receptor class B type I deficient mice.

Applications

Species

Mouse

Jia Cao,Yanyong Xu,Feifei Li,Liang Shang,Daping Fan,Hong Yu

Stem cell reports 10:1384-1397 PubMed29641991

2018

PLAG1 and USF2 Co-regulate Expression of Musashi-2 in Human Hematopoietic Stem and Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Muluken S Belew,Sonam Bhatia,Ava Keyvani Chahi,Stefan Rentas,Jonathan S Draper,Kristin J Hope

Nutrition & diabetes 6:e206 PubMed27110687

2016

Liver-specific overexpression of LPCAT3 reduces postprandial hyperglycemia and improves lipoprotein metabolic profile in mice.

Applications

Unspecified application

Species

Unspecified reactive species

J G Cash,D Y Hui

Journal of the American Society of Nephrology : JA 25:1028-36 PubMed24357674

2013

Cubilin maintains blood levels of HDL and albumin.

Applications

Unspecified application

Species

Mouse

Obaidullah Aseem,Brian T Smith,Marion A Cooley,Brent A Wilkerson,Kelley M Argraves,Alan T Remaley,W Scott Argraves

Journal of lipid research 55:41-52 PubMed24186947

2013

Hepatic trans-Golgi action coordinated by the GTPase ARFRP1 is crucial for lipoprotein lipidation and assembly.

Applications

Species

Mouse

Deike Hesse,Katrin Radloff,Alexander Jaschke,Merit Lagerpusch,Bomee Chung,Anne Tailleux,Bart Staels,Annette Schürmann

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apolipoprotein A I antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise