Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) is a rabbit monoclonal antibody that is used to detect Apolipoprotein A I in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ELISA. Suitable for Human, samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 40 publications
-Over 40 publications
Images
![Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--western-blot-img74298.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102664.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945)")
![Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--western-blot-img97848.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945)")
![Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--immunocytochemistry-immunofluorescence-img76830.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (AB52945)")
Publications
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- Scientific reports 13:200192023Transthyretin amyloid deposition in ligamentum flavum (LF) is significantly correlated with LF and epidural fat hypertrophy in patients with lumbar spinal stenosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuya Maeda,Kazuki Sugimoto,Masayoshi Tasaki,Takuya Taniwaki,Takahiro Arima,Yuto Shibata,Makoto Tateyama,Tatsuki Karasugi,Takanao Sueyoshi,Tetsuro Masuda,Yusuke Uehara,Takuya Tokunaga,Satoshi Hisanaga,Masaki Yugami,Ryuji Yonemitsu,Katsumasa Ideo,Kozo Matsushita,Yuko Fukuma,Masaru Uragami,Junki Kawakami,Naoto Yoshimura,Kosei Takata,Masaki Shimada,Shuntaro Tanimura,Hideto Matsunaga,Yuki Kai,Shu Takata,Ryuta Kubo,Rui Tajiri,Fuka Homma,Xiao Tian,Mitsuharu Ueda,Takayuki Nakamura,Takeshi MiyamotoPubMed 37973808
- PloS one 18:e02916232023Extracellular vesicles as a potential source of tumor-derived DNA in advanced pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMorten Lapin,Kjersti Tjensvoll,Karoline Nedrebø,Eline Taksdal,Hans Janssen,Bjørnar Gilje,Oddmund NordgårdPubMed 37708210
- Precision clinical medicine 6:pbad0212023Impact of apolipoprotein A1 on tumor immune microenvironment, clinical prognosis and genomic landscape in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Wang,Shipeng Chen,Xiao Xiao,Fan Yang,Jinhan Wang,Hui Zong,Yuzhen Gao,Chenjun Huang,Xuewen Xu,Meng Fang,Xiaoyan Zhang,Chunfang GaoPubMed 38025972
- Science advances 9:eade02932023Secreted endogenous macrosomes reduce Aβ burden and ameliorate Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCunli Wang,Yiming Yang,Xiaoyu Zhang,Zhenqiang Shi,Huiling Gao,Manli Zhong,Yonggang Fan,Hongyan Zhang,Bo Liu,Guangyan QingPubMed 37235655
- British journal of pharmacology 180:1999-20172023Therapeutic effect of human ApoA-I-Milano variant in aged transgenic mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMontse Solé,Paula Marazuela,Laura Castellote,Anna Bonaterra-Pastra,Lydia Giménez-Llort,Mar Hernández-GuillamonPubMed 36872299
- Journal of extracellular vesicles 12:e123082023Extracellular vesicles and high-density lipoproteins: Exercise and oestrogen-responsive small RNA carriers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSira Karvinen,Tia-Marje Korhonen,Tero Sievänen,Jari E Karppinen,Hanna-Kaarina Juppi,Veera Jakoaho,Urho M Kujala,Jari A Laukkanen,Maarit Lehti,Eija K LaakkonenPubMed 36739598
- Nature communications 14:5572023Quantifying PON1 on HDL with nanoparticle-gated electrokinetic membrane sensor for accurate cardiovascular risk assessment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonu Kumar,Nalin Maniya,Ceming Wang,Satyajyoti Senapati,Hsueh-Chia ChangPubMed 36732521
- iScience 26:1060482023Differential proteomic patterns of plasma extracellular vesicles show potential to discriminate β-thalassemia subtypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Li,Bowen Wu,Jifeng Wang,Yumeng Yan,Peng An,Yuezhen Li,Yuning Liu,Yanfei Hou,Xiaoqing Qing,Lili Niu,Xiang Ding,Zhensheng Xie,Mengmeng Zhang,Xiaojing Guo,Xiulan Chen,Tanxi Cai,Jianming Luo,Fudi Wang,Fuquan YangPubMed 36824279
- Communications biology 5:13582022Electrodeposited magnetic nanoporous membrane for high-yield and high-throughput immunocapture of extracellular vesicles and lipoproteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenguang Zhang,Xiaoye Huo,Yini Zhu,James N Higginbotham,Zheng Cao,Xin Lu,Jeffrey L Franklin,Kasey C Vickers,Robert J Coffey,Satyajyoti Senapati,Ceming Wang,Hsueh-Chia ChangPubMed 36496485
- Cell communication and signaling : CCS 20:1912022UBE2O ubiquitinates PTRF/CAVIN1 and inhibits the secretion of exosome-related PTRF/CAVIN1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaotong Cen,Qing Chen,Bin Wang,Hongjie Xu,Xu Wang,Yixia Ling,Xiaofei Zhang,Dajiang QinPubMed 36443833
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | ELISA | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Tested | Tested |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/60 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes Antibody conc. = 0 - 1000 ng/ml |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes For unpurified use at 1/20000 dilution. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
1 product for Alternative Version
Target data
Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Alternative names
Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1
Recommended products
Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) is a rabbit monoclonal antibody that is used to detect Apolipoprotein A I in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ELISA. Suitable for Human, samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 40 publications
-Over 40 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1368Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Notes
Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, samples.
What is the molecular weight of Apolipoprotein A I?
Anti-Apolipoprotein A I [EP1368Y] (ab52945) specifically detects a band for Apolipoprotein A I (UniProt: P02647) at a molecular weight of 31kDa.
Trusted by the scientific community
Anti-Apolipoprotein A I [EP1368Y] (ab52945) was first used in a scientific publication in 2007 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Apolipoprotein A I [EP1368Y] (ab52945) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EP1368Y] also available for your convenience:
ab52945, Carrier free - Anti-Apolipoprotein A I antibody [EP1368Y] - Low endotoxin, Azide free ab185132, Carrier free - Anti-Apolipoprotein A I antibody [EP1368Y] - BSA and Azide free ab271849, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Apolipoprotein A I antibody [EP1368Y] ab279676, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Apolipoprotein A I antibody [EP1368Y] ab279677, PE - PE Anti-Apolipoprotein A I antibody [EP1368Y] ab282174
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
- Biological function summary
ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.
- Pathways
The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.
- Associated diseases and disorders
Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--western-blot-img74298.jpg "Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
All lanes: Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution
All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 31 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102664.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Apolipoprotein A I with purified ab52945 at 1/100 dilution (1.95 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--western-blot-img97848.jpg "Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
All lanes: Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution
All lanes: Human liver lysates at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 31 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--immunocytochemistry-immunofluorescence-img76830.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/250 dilution (0.8 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--western-blot-img28592.gif "Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
All lanes: Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/20000 dilution
All lanes: fetal liver lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 31 kDa
![Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--immunoprecipitation-img150737.jpg "Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
ab52945 (purified) at 1/20 dilution (1ug) immunoprecipitating Apolipoprotein A I in Human fetal liver lysates.
Lane 1: Human fetal liver lysates 10ug
Lane 2 (+): ab52945 & Human fetal liver lysates
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52945 in Human fetal liver lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
Predicted band size: 30 kDa
![Indirect ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--indirect-elisa-img22690.jpg "Indirect ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Indirect ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
ELISA analysis of Apolipoprotein A I recombinant protein at 1000 ng/mL with ab52945 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138182.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.
![Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--immunocytochemistry-immunofluorescence-img10302.gif "Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.
![Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--flow-cytometry-intracellular-img415071.jpg "Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--immunoprecipitation-img150737.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
![Indirect ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--indirect-elisa-img22690.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
![Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-ep1368y-ab52945--flow-cytometry-intracellular-img415071.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
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