Rabbit Recombinant Monoclonal Apolipoprotein A I antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Transfected cell line samples.
Images
![Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (AB307090), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-epr25446-111-bsa-and-azide-free-ab307090--immunohistochemistry-img404985.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (AB307090)")
![Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (AB307090), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-epr25446-111-bsa-and-azide-free-ab307090--immunohistochemistry-img404983.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (AB307090)")
![Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (AB307090), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-epr25446-111-bsa-and-azide-free-ab307090--immunohistochemistry-img404984.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (AB307090)")
![Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (AB307090), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-epr25446-111-bsa-and-azide-free-ab307090--immunohistochemistry-img404986.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (AB307090)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | WB | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
| Transfected cell line | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell line | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Transfected cell line | Dilution info - | Notes - |
Target data
Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Alternative names
Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, Apoa1
Recommended products
Rabbit Recombinant Monoclonal Apolipoprotein A I antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Transfected cell line samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25446-111
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
- Biological function summary
ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.
- Pathways
The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.
- Associated diseases and disorders
Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-epr25446-111-bsa-and-azide-free-ab307090--immunohistochemistry-img404985.jpg "Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090)")
Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090)
This data was developed using Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Apolipoprotein A I with Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089 at 1/100 (4.63 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on plasma of mouse spleen. The section was incubated with Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-epr25446-111-bsa-and-azide-free-ab307090--immunohistochemistry-img404983.jpg "Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090)")
Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090)
This data was developed using Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Apolipoprotein A I with Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089 at 1/100 (4.63 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on plasma of mouse cardiac muscle. The section was incubated with Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-epr25446-111-bsa-and-azide-free-ab307090--immunohistochemistry-img404984.jpg "Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090)")
Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090)
This data was developed using Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Apolipoprotein A I with Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089 at 1/100 (4.63 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on plasma of mouse liver. The section was incubated with Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090), expandable thumbnail](https://content.abcam.com/products/images/apolipoprotein-a-i-antibody-epr25446-111-bsa-and-azide-free-ab307090--immunohistochemistry-img404986.jpg "Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090)")
Immunohistochemistry - Anti-Apolipoprotein A I antibody [EPR25446-111] - BSA and Azide free (ab307090)
This data was developed using Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded transfected HEK-293T cells labeling Apolipoprotein A I with Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089 at 1/100 (4.63 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a Apoa1 expression vector, no staining on (B) HEK-293T transfected with empty vector.
The section was incubated with Anti-Apolipoprotein A I antibody [EPR25446-111] ab307089 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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