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Rabbit Recombinant Monoclonal Apolipoprotein A I antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Rat samples.

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Images

Western blot - Anti-Apolipoprotein A I antibody [EPR28749-505] (AB323863), expandable thumbnail
  • Western blot - Anti-Apolipoprotein A I antibody [EPR28749-505] (AB323863), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EPR28749-505] (AB323863), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EPR28749-505] (AB323863), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EPR28749-505] (AB323863), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PWB
Mouse
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Tested

Tested
Tested

Species
Rat
Dilution info
1/2000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Target data

Alternative names

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1

Rabbit Recombinant Monoclonal Apolipoprotein A I antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28749-505
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863), expandable thumbnail

    Western blot - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863)

    Apolipoprotein A I Western blot staining using rabbit Anti-Apolipoprotein A I antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863) at 1/1000 dilution

    Lane 1: McA-RH7777 (rat liver epithelial cell) whole cell lysate at 20 µg

    Lane 2: McA-RH7777 culture supernatant at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 30 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863), expandable thumbnail

    Western blot - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863)

    Apolipoprotein A I Western blot staining using rabbit Anti-Apolipoprotein A I antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 23969698, 23409054).

    Low expression: liver, skeletal muscle (HPA database); brain (PMID: 1478650); lung (PMID: 23409054).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863) at 1/1000 dilution

    Lane 1: Rat serum lysate at 20 µg

    Lane 2: Rat plasma lysate at 20 µg

    Lane 3: Rat liver tissue lysate at 20 µg

    Lane 4: Rat brain tissue lysate at 20 µg

    Lane 5: Rat lung tissue lysate at 20 µg

    Lane 6: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 28 kDa, 36 kDa

    Exposure time: 26s

  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863)

    Apolipoprotein A I Immunocytochemistry/ Immunofluorescence staining of McA-RH7777 (rat liver epithelial cell) using rabbit Anti-Apolipoprotein A I antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized McA-RH7777 (rat liver epithelial cell) cells labelling Apolipoprotein A I with ab323863 at 1/2000 (0.256 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in McA-RH7777 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863)

    Apolipoprotein A I Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Apolipoprotein A I antibody

    Immunohistochemical analysis of paraffin-embedded (A) Rat non-perfused fixed liver and (B) Rat perfused fixed liver tissue labeling Apolipoprotein A I with ab323863 at 1/2000 (0.256 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plasma in (A) rat non-perfused fixed liver, negative on (B) rat perfused fixed liver. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EPR28749-505] (ab323863)

    Apolipoprotein A I Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of plasma in rat non-perfused fixed kidney tissue using rabbit Anti-Apolipoprotein A I antibody

    Immunohistochemical analysis of paraffin-embedded Rat non-perfused fixed kidney tissue labeling Apolipoprotein A I with ab323863 at 1/2000 (0.256 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plasma in rat non-perfused fixed kidney. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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