AB92478

Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913]

Name: Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (ab92478) | Abcam
Brand: Abcam Limited
SKU: AB92478
Price: 335 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(11 Publications)

Rabbit Recombinant Monoclonal Apolipoprotein A II/ApoA-II antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications.

View Alternative Names

Apolipoprotein A-II, Apo-AII, ApoA-II, Apolipoprotein A2, APOA2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (AB92478)

ICC/IF

AbReview56149****

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (AB92478)

Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein A II/ApoA-II with ab92478 at 1/500. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated monoclonal donkey antibody at 1/3000 dilution was used as the secondary antibody.

This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (AB92478)

ab92478 at 1/100 dilution staining Apolipoprotein A II/ApoA-II in HepG2 cells by immunofluorescence.

Unknown

Western blot - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (AB92478)

All lanes:

Western blot - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (ab92478) at 1/1000 dilution

All lanes:

Human plasma lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Carrier free

Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2913

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-II (also known as ApoA-II or Apolipoprotein A2) is a pivotal protein component of high-density lipoproteins (HDL) in the plasma. It is encoded by the APOA2 gene in humans and displays a molecular mass of approximately 17.4 kDa. Apolipoprotein A-II is primarily expressed in the liver and secreted into the bloodstream where it plays a direct role in lipid metabolism. Its structural composition includes a highly amphipathic α-helical structure that facilitates interaction with lipids aiding in lipoprotein formation and stability.

Biological function summary

Apolipoprotein A-II serves as a significant regulator of HDL metabolism and lipid transport. It is an integral part of HDL particles contributing to their stability and function. Apolipoprotein A-II influences lecithin-cholesterol acyltransferase (LCAT) activity which affects cholesterol esterification. Although less studied than its counterpart Apolipoprotein A-I ApoA-II's interaction with lipids and proteins such as CETP (cholesteryl ester transfer protein) suggests its involvement in lipid exchange and reverse cholesterol transport processes.

Pathways

Apolipoprotein A-II impacts the reverse cholesterol transport pathway and lipid metabolic processes. ApoA-II works closely with its relative Apolipoprotein A-I both part of the same HDL complex. Together they play essential roles in maintaining cholesterol homeostasis and promoting cholesterol efflux from cells back to the liver. The mechanism by which it modulates the function of lipoprotein lipase and hepatic lipase further implicates its role in lipid metabolism and energy balance processes.

Dysfunction or abnormal levels of Apolipoprotein A-II have associations with cardiovascular diseases and metabolic syndromes. Altered ApoA-II levels correlate with an increased risk of atherosclerosis as its imbalance can drive perturbations in HDL composition and function. Moreover research links Apolipoprotein A-II with insulin resistance linking it with ApoA-I imbalanced lipid transport and metabolism in obesity and diabetes contexts. Understanding these connections may guide therapeutic strategies targeting ApoA-II to mitigate the risks and progression of these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

See full target information APOA2

style

left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Biology 14: PubMed40136514

2025

Temporal Profiling of Cellular and Molecular Processes in Osteodifferentiation of Dental Pulp Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bibiána Baďurová,Kristina Nystøl,Terézia Okajček Michalič,Veronika Kucháriková,Dagmar Statelová,Slavomíra Nováková,Ján Strnádel,Erika Halašová,Henrieta Škovierová

BMC pregnancy and childbirth 25:21 PubMed39789492

2025

High taurocholic acid concentration induces ferroptosis by downregulating FTH1 expression in intrahepatic cholestasis of pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Jian Zeng,Hua-Jing Yang,Ying-Jie Gu,Meng-Nan Yang,Meng-Ru Sun,Sheng-Kai Cheng,Yan-Yan Hou,Wei Gu

Reproductive sciences (Thousand Oaks, Calif.) 31:1573-1585 PubMed38177949

2024

Proteomic Biomarkers of Intrahepatic Cholestasis of Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Weijian Zeng,Yanyan Hou,Wei Gu,Zheng Chen

Nature communications 14:4007 PubMed37414857

2023

Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their function.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liu,Ralf Nilsson,Elisa Lázaro-Ibáñez,Hanna Duàn,Tasso Miliotis,Marie Strimfors,Michael Lerche,Ana Rita Salgado Ribeiro,Johan Ulander,Daniel Lindén,Anna Salvati,Alan Sabirsh

Science advances 9:eade0293 PubMed37235655

2023

Secreted endogenous macrosomes reduce Aβ burden and ameliorate Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Cunli Wang,Yiming Yang,Xiaoyu Zhang,Zhenqiang Shi,Huiling Gao,Manli Zhong,Yonggang Fan,Hongyan Zhang,Bo Liu,Guangyan Qing

International journal of molecular sciences 22: PubMed34360674

2021

Comparative Proteomic and Metabolomic Analysis of Human Osteoblasts, Differentiated from Dental Pulp Stem Cells, Hinted Crucial Signaling Pathways Promoting Osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Slavomíra Nováková,Maksym Danchenko,Terézia Okajčeková,Eva Baranovičová,Andrej Kováč,Marián Grendár,Gábor Beke,Janka Pálešová,Ján Strnádel,Mária Janíčková,Erika Halašová,Henrieta Škovierová

Journal of atherosclerosis and thrombosis 28:730-741 PubMed32999208

2021

Glycation of HDL Polymerizes Apolipoprotein M and Attenuates Its Capacity to Bind to Sphingosine 1-Phosphate.

Applications

Unspecified application

Species

Unspecified reactive species

Tamaki Kobayashi,Makoto Kurano,Mai Nanya,Tomo Shimizu,Ryunosuke Ohkawa,Minoru Tozuka,Yutaka Yatomi

The Journal of biological chemistry 294:19022-19033 PubMed31666337

2019

Modified sites and functional consequences of 4-oxo-2-nonenal adducts in HDL that are elevated in familial hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Linda S May-Zhang,Valery Yermalitsky,John T Melchior,Jamie Morris,Keri A Tallman,Mark S Borja,Tiffany Pleasent,Venkataraman Amarnath,Wenliang Song,Patricia G Yancey,W Sean Davidson,MacRae F Linton,Sean S Davies

Biomedicine & pharmacotherapy = Biomedecine & phar 118:109251 PubMed31351426

2019

PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhao,Xiuli Zheng,Jingfang Liu,Rong Zheng,Rui Yang,Ying Wang,Lifang Sun

Journal of lipid research 58:2220-2228 PubMed28893842

2017

HDL structure and function is profoundly affected when stored frozen in the absence of cryoprotectants.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Holzer,Sabine Kern,Markus Trieb,Athina Trakaki,Gunther Marsche

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913]

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (AB92478)

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (AB92478)

Western blot - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (AB92478)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise